The live-cell biomolecular dynamics refers to the rapid movement,interacting with their surroundings,and the transformation of biomolecules within living cells.Deeper understanding of biomolecular dynamics in their na...The live-cell biomolecular dynamics refers to the rapid movement,interacting with their surroundings,and the transformation of biomolecules within living cells.Deeper understanding of biomolecular dynamics in their native state may reveal fundamental molecular mechanisms of cell function and disease formation[1].Also,understanding the disease associated biomolecular dynamics can assist in disease diagnosis and therapy.展开更多
DNA tetrahedral nanostructures are considered to be uew nanocarriers because they can be precisely controlled and hold excellent penetration ability to the cellular membrane. Although the DNA tetrahedral nanostructure...DNA tetrahedral nanostructures are considered to be uew nanocarriers because they can be precisely controlled and hold excellent penetration ability to the cellular membrane. Although the DNA tetrahedral nanostructure is extensively studied in biology and medicine, its behavior in the cells with nanoscale resolution is not understood clearly. In this letter, we demonstrate superrcsolution fluorescence imaging of the distribution of DNA tetrahedral nanostructures in the cell with a simulated emission depletion （STED） microscope, which is built based on a conventional eonfocal microscope and can t）rovide a resolution of 70 nm.展开更多
基金supported by the National Key R&D Program of China(2022YFA1603600)the National Natural Science Foundation of China(22122406,22104089,and 22204106)+2 种基金the Shenzhen Medical Research Fund(B2301003)the Guangdong Basic and Applied Basic Research Foundation(2021A1515110710)the Guangdong Provincial Pearl River Talents Program(2021QN02Z631)。
文摘The live-cell biomolecular dynamics refers to the rapid movement,interacting with their surroundings,and the transformation of biomolecules within living cells.Deeper understanding of biomolecular dynamics in their native state may reveal fundamental molecular mechanisms of cell function and disease formation[1].Also,understanding the disease associated biomolecular dynamics can assist in disease diagnosis and therapy.
基金supported by the National Natural Science Foundation of China under Grand Nos.61008056,21227804,61078016,and 61378062)
文摘DNA tetrahedral nanostructures are considered to be uew nanocarriers because they can be precisely controlled and hold excellent penetration ability to the cellular membrane. Although the DNA tetrahedral nanostructure is extensively studied in biology and medicine, its behavior in the cells with nanoscale resolution is not understood clearly. In this letter, we demonstrate superrcsolution fluorescence imaging of the distribution of DNA tetrahedral nanostructures in the cell with a simulated emission depletion （STED） microscope, which is built based on a conventional eonfocal microscope and can t）rovide a resolution of 70 nm.
