【目的】探讨针药结合对抑郁症睡眠障碍(SDD)大鼠行为学变化及对中缝背核(NRD)5-羟色胺受体5-HT1AR、5-HT2AR m RNA表达的影响。【方法】正常组8只SD大鼠常规合笼饲养,不接受任何刺激与处置。32只SD大鼠接受为期21 d的孤养+慢性不可预...【目的】探讨针药结合对抑郁症睡眠障碍(SDD)大鼠行为学变化及对中缝背核(NRD)5-羟色胺受体5-HT1AR、5-HT2AR m RNA表达的影响。【方法】正常组8只SD大鼠常规合笼饲养,不接受任何刺激与处置。32只SD大鼠接受为期21 d的孤养+慢性不可预见中等应激(CUMS)法+快速动眼相(REM)睡眠剥夺法造模,其中28只顺利完成造模,第2次分组为模型组、针刺组、药物组、针药组,每组各7只。模型组仅造模不干预;针刺组取印堂、神庭配太冲(双)进行针刺,各穴刺激15 s,隔10 min运针1次,每次留针20 min;药物组给予0.83 mg·kg-1·d-1舍曲林和0.067 mg·kg-1·d-1阿普唑仑水溶液灌胃;针药组同时结合针刺组与药物组的2种干预方式;3组均连续干预7 d。【结果】造模的第7、14、21天,与正常组比较,模型组大鼠体质量、Open-field得分、蔗糖偏好值均显著降低(P<0.05);第28天,各干预组体质量、Open-field得分都较模型组显著升高(P<0.05),针药组的Open-field得分提升与针刺组和药物组比较差异均有统计学意义(P<0.05);仅针药组的蔗糖偏好值得到显著提升(P<0.05),且仅针药组的NRD 5-HT1AR m RNA表达显著升高(P<0.05),3个干预组的NRD 5-HT2AR m RNA表达均较模型组显著下调(P<0.05)。【结论】为期7 d的针药结合干预对SDD大鼠起效较快,能明显改善抗抑郁药物起效慢的问题。展开更多
Objective:To explore whether auricular concha eletroacupuncture(ACEA)is effective in regulating lipidlipoprotein metabolism in rats submitted to cold stress.Methods:Thirty-six adult male Sprague-Dawley rats were place...Objective:To explore whether auricular concha eletroacupuncture(ACEA)is effective in regulating lipidlipoprotein metabolism in rats submitted to cold stress.Methods:Thirty-six adult male Sprague-Dawley rats were placed in four groups(9 rats in each group),the rats in three groups of which were submitted to cold stress for fourteen days,the last one of which was a control group.After the cold stress process,in those three groups,the rats of one group were with no treatment(stress only),two were treated with either ACEA or auricular margin eletroacupuncture(AMEA)repeated for fourteen days.On the 14th day,all the rats were sacrificed after all experimental procedure for blood sampling.Blood glucose,triglycerides(TG),total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C)and low-density lipoprotein cholesterol(LDL-C)levels were tested by using the collected serum.Plasma lecithin-cholesterol acyltransferase(LCAT)was measured with ELISA kit.Results:ACEA down-regulated the TG level(P<0.05)and LDL-C level(P<0.05),and up-regulated HDL-C level(P<0.05)and LACT level(P<0.05).AMEA did not regulate the bio-markers.Conclusion:ACEA played an important role in regulating lipid-lipoprotein metabolism in rats submitted to cold stress.展开更多
基金北京市科委国家重大研发计划(Z161100002616003):基于耳甲-迷走神经联系治疗抑郁症可穿戴设备从基础到临床研究负责人:荣培晶+9 种基金中德科学中心中德科学基金(GZ1236):Purine signaling in depression and antidepressant effect of transcutaneous auricular vagus electroacupuncture负责人:荣培晶国家自然科学基金委面上项目(81473780):耳甲-迷走神经刺激治疗失眠的临床及机制研究负责人:荣培晶中国博士后科学基金会(2016M590185):基于1H-NMR代谢组学研究耳甲迷走神经刺激的抗抑郁机制负责人:俞裕天财政部中央级公益性科研院所基本科研业务费专项资金(ZZ16012):电针耳甲对意识障碍患者脑功能促进作用研究负责人:俞裕天北京市自然科学基金委重点项目(ZG105):耳甲-迷走神经联系与耳针治疗抑郁症:从基础到临床负责人:荣培晶
基金Supported by Major National R&D Program of China:Z161100002616003Joint Sino-German-Project:GZ1236+2 种基金The Fundamental Research Funds for the Central Public Welfare Research Institutes:ZZ16012Chinese Postdoctoral Science Foundation:2016M590185Natural Science Foundation of Beijing:7111007。
文摘Objective:To explore whether auricular concha eletroacupuncture(ACEA)is effective in regulating lipidlipoprotein metabolism in rats submitted to cold stress.Methods:Thirty-six adult male Sprague-Dawley rats were placed in four groups(9 rats in each group),the rats in three groups of which were submitted to cold stress for fourteen days,the last one of which was a control group.After the cold stress process,in those three groups,the rats of one group were with no treatment(stress only),two were treated with either ACEA or auricular margin eletroacupuncture(AMEA)repeated for fourteen days.On the 14th day,all the rats were sacrificed after all experimental procedure for blood sampling.Blood glucose,triglycerides(TG),total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C)and low-density lipoprotein cholesterol(LDL-C)levels were tested by using the collected serum.Plasma lecithin-cholesterol acyltransferase(LCAT)was measured with ELISA kit.Results:ACEA down-regulated the TG level(P<0.05)and LDL-C level(P<0.05),and up-regulated HDL-C level(P<0.05)and LACT level(P<0.05).AMEA did not regulate the bio-markers.Conclusion:ACEA played an important role in regulating lipid-lipoprotein metabolism in rats submitted to cold stress.
