目的:间充质干细胞移植作为具有发展前景的脊髓损伤治疗方法之一,目前大多集中于小动物模型相关研究,大动物实验开展较少,并且对干细胞的种类选择与治疗效果仍存在争议。文章通过Meta分析间充质干细胞对大动物模型脊髓损伤相关指标的影...目的:间充质干细胞移植作为具有发展前景的脊髓损伤治疗方法之一,目前大多集中于小动物模型相关研究,大动物实验开展较少,并且对干细胞的种类选择与治疗效果仍存在争议。文章通过Meta分析间充质干细胞对大动物模型脊髓损伤相关指标的影响,评价其对脊髓损伤修复的作用。方法:计算机检索PubMed、Cochrane、OVID、Web of Science和中国知网,检索时限从建库起至2019年12月,收集关于间充质干细胞治疗大动物脊髓损伤的系列研究。由2位研究者按照纳入标准独立完成文献筛选、资料提取及方法学质量评价,采用Stata16.0进行Meta分析。结果:最终10篇文献纳入研究,Meta分析结果显示:①间充质干细胞可显著改善脊髓损伤后运动功能[I2=97.73%,MD=3.94,95%CI(2.15,5.72),P<0.01],并根据细胞来源、观察时间、移植时机、移植方式和移植类型分不同进行亚组分析:骨髓间充质干细胞组、非骨髓间充质干细胞组、短时间观察组(<2个月)、长时间观察组(≥2个月)、急性期移植组、非急性期移植组、单纯细胞移植组、异种移植组的运动评分明显高于对照组(P<0.01);而支架复合移植组运动评分相较对照组,差异无显著性意义(P>0.05);②间充质干细胞治疗组的损伤面积明显小于对照组[I2=98.05%,MD=-1.00,95%CI(-1.95,-0.04),P=0.04];③间充质干细胞治疗组与对照组之间胶质纤维酸性蛋白相对表达量差异无显著性意义[I2=99.48%,MD=80.61,95%CI(-27.48,188.70),P=0.14]。结论:间充质干细胞移植治疗对大动物脊髓损伤的运动功能和损伤修复有明显改善作用,且安全性较高。受纳入文献质量的限制,以上结论需更高质量、更大样本的随机对照实验加以验证。展开更多
Multi-soliton solution to a multi-component coupled Ito system is investigated based on the Hirota bilinear method. By virtue of the perturbation method, we firstly derive one- and two-soliton solutions for the couple...Multi-soliton solution to a multi-component coupled Ito system is investigated based on the Hirota bilinear method. By virtue of the perturbation method, we firstly derive one- and two-soliton solutions for the coupled Ito system possessing four components. Then the multi-soliton solution for the multi-component coupled Ito system is summarized into a general form expressed by pfaffians. Finally, this general pfaffian-type soliton solution is proved by pfaffian techniques.展开更多
文摘目的:间充质干细胞移植作为具有发展前景的脊髓损伤治疗方法之一,目前大多集中于小动物模型相关研究,大动物实验开展较少,并且对干细胞的种类选择与治疗效果仍存在争议。文章通过Meta分析间充质干细胞对大动物模型脊髓损伤相关指标的影响,评价其对脊髓损伤修复的作用。方法:计算机检索PubMed、Cochrane、OVID、Web of Science和中国知网,检索时限从建库起至2019年12月,收集关于间充质干细胞治疗大动物脊髓损伤的系列研究。由2位研究者按照纳入标准独立完成文献筛选、资料提取及方法学质量评价,采用Stata16.0进行Meta分析。结果:最终10篇文献纳入研究,Meta分析结果显示:①间充质干细胞可显著改善脊髓损伤后运动功能[I2=97.73%,MD=3.94,95%CI(2.15,5.72),P<0.01],并根据细胞来源、观察时间、移植时机、移植方式和移植类型分不同进行亚组分析:骨髓间充质干细胞组、非骨髓间充质干细胞组、短时间观察组(<2个月)、长时间观察组(≥2个月)、急性期移植组、非急性期移植组、单纯细胞移植组、异种移植组的运动评分明显高于对照组(P<0.01);而支架复合移植组运动评分相较对照组,差异无显著性意义(P>0.05);②间充质干细胞治疗组的损伤面积明显小于对照组[I2=98.05%,MD=-1.00,95%CI(-1.95,-0.04),P=0.04];③间充质干细胞治疗组与对照组之间胶质纤维酸性蛋白相对表达量差异无显著性意义[I2=99.48%,MD=80.61,95%CI(-27.48,188.70),P=0.14]。结论:间充质干细胞移植治疗对大动物脊髓损伤的运动功能和损伤修复有明显改善作用,且安全性较高。受纳入文献质量的限制,以上结论需更高质量、更大样本的随机对照实验加以验证。
基金Supported by the China Scholarship Council, the Global Change Research Program of China under Grant No 2015CB953904, the National Natural Science Foundation of China under Grant Nos 11275072 and 11435005, the Research Fund for the Doctoral Program of Higher Education of China under Grant No 20120076110024, the Innovative Research Team Program of the National Natural Science Foundation of China under Grant No 61321064, the Shanghai Knowledge Service Platform for Trustworthy Internet of Things under Grant No ZF1213, the Shanghai Minhang District Talents of High Level Scientific Research Project, and the Talent Fund and K.C. Won~ Mazna Fund in Nin~bo University.
文摘Multi-soliton solution to a multi-component coupled Ito system is investigated based on the Hirota bilinear method. By virtue of the perturbation method, we firstly derive one- and two-soliton solutions for the coupled Ito system possessing four components. Then the multi-soliton solution for the multi-component coupled Ito system is summarized into a general form expressed by pfaffians. Finally, this general pfaffian-type soliton solution is proved by pfaffian techniques.
