In this study, Saccharomyces cerevisiae (baker's yeast) was produced in a fed-batch bioreactor at the optimal dissolved oxygen concentration (DOC) and growth medium temperature. However, it is very difficult to co...In this study, Saccharomyces cerevisiae (baker's yeast) was produced in a fed-batch bioreactor at the optimal dissolved oxygen concentration (DOC) and growth medium temperature. However, it is very difficult to control the DOC using conventional controllers because of the poorly understood and constantly changing dynamics of the bioprocess. A generalized predictive controller (GPC) based on a nonlinear autoregressive integrated moving average exogenous (NARIMAX) model is presented to stabilize the DOC by manipulation of air flow rate. The NARIMAX model is built by an improved recursive least-squares support vector machine, which is trained by an in-place computation scheme and avoids the computation of the inverse of a large matrix and memory reallocation. The proposed nonlinear GPC algorithm requires little preliminary knowledge of the fermentation process, and directly obtains the nonlinear model in matrix form by using iterative multiple modeling instead of linearization at each sampling period. By application of an on-line bioreactor control, experimental results demonstrate the robustness, effectiveness and advantages of the new controller.展开更多
State estimation is the precondition and foundation of a bioprocess monitoring and optimal control. However,there are many difficulties in dealing with a non-linear system,such as the instability of process, un-modele...State estimation is the precondition and foundation of a bioprocess monitoring and optimal control. However,there are many difficulties in dealing with a non-linear system,such as the instability of process, un-modeled dynamics,parameter sensitivity,etc.This paper discusses the principles and characteristics of three different approaches,extended Kalman filters,strong tracking filters and unscented transformation based Kalman filters.By introducing the unscented transformation method and a sub-optimal fading factor to correct the prediction error covariance,an improved Kalman filter,unscented transformation based robust Kalman filter,is proposed. The performance of the algorithm is compared with the strong tracking filter and unscented transformation based Kalman filter and illustrated in a typical case study for glutathione fermentation process.The results show that the proposed algorithm presents better accuracy and stability on the state estimation in numerical calculations.展开更多
Support vector machine(SVM) has shown great potential in pattern recognition and regressive estima-tion.Due to the industrial development demands,such as the fermentation process modeling,improving the training perfor...Support vector machine(SVM) has shown great potential in pattern recognition and regressive estima-tion.Due to the industrial development demands,such as the fermentation process modeling,improving the training performance on increasingly large sample sets is an important problem.However,solving a large optimization problem is computationally intensive and memory intensive.In this paper,a geometric interpretation of SVM re-gression(SVR) is derived,and μ-SVM is extended for both L1-norm and L2-norm penalty SVR.Further,Gilbert al-gorithm,a well-known geometric algorithm,is modified to solve SVR problems.Theoretical analysis indicates that the presented SVR training geometric algorithms have the same convergence and almost identical cost of computa-tion as their corresponding algorithms for SVM classification.Experimental results show that the geometric meth-ods are more efficient than conventional methods using quadratic programming and require much less memory.展开更多
基金Supported by the National Natural Science Foundation of China (20476007, 20676013)
文摘In this study, Saccharomyces cerevisiae (baker's yeast) was produced in a fed-batch bioreactor at the optimal dissolved oxygen concentration (DOC) and growth medium temperature. However, it is very difficult to control the DOC using conventional controllers because of the poorly understood and constantly changing dynamics of the bioprocess. A generalized predictive controller (GPC) based on a nonlinear autoregressive integrated moving average exogenous (NARIMAX) model is presented to stabilize the DOC by manipulation of air flow rate. The NARIMAX model is built by an improved recursive least-squares support vector machine, which is trained by an in-place computation scheme and avoids the computation of the inverse of a large matrix and memory reallocation. The proposed nonlinear GPC algorithm requires little preliminary knowledge of the fermentation process, and directly obtains the nonlinear model in matrix form by using iterative multiple modeling instead of linearization at each sampling period. By application of an on-line bioreactor control, experimental results demonstrate the robustness, effectiveness and advantages of the new controller.
基金Supported by the National Natural Science Foundation of China （20476007, 20676013）.
文摘State estimation is the precondition and foundation of a bioprocess monitoring and optimal control. However,there are many difficulties in dealing with a non-linear system,such as the instability of process, un-modeled dynamics,parameter sensitivity,etc.This paper discusses the principles and characteristics of three different approaches,extended Kalman filters,strong tracking filters and unscented transformation based Kalman filters.By introducing the unscented transformation method and a sub-optimal fading factor to correct the prediction error covariance,an improved Kalman filter,unscented transformation based robust Kalman filter,is proposed. The performance of the algorithm is compared with the strong tracking filter and unscented transformation based Kalman filter and illustrated in a typical case study for glutathione fermentation process.The results show that the proposed algorithm presents better accuracy and stability on the state estimation in numerical calculations.
基金Supported by the National Natural Science Foundation of China (20476007,20676013)
文摘Support vector machine(SVM) has shown great potential in pattern recognition and regressive estima-tion.Due to the industrial development demands,such as the fermentation process modeling,improving the training performance on increasingly large sample sets is an important problem.However,solving a large optimization problem is computationally intensive and memory intensive.In this paper,a geometric interpretation of SVM re-gression(SVR) is derived,and μ-SVM is extended for both L1-norm and L2-norm penalty SVR.Further,Gilbert al-gorithm,a well-known geometric algorithm,is modified to solve SVR problems.Theoretical analysis indicates that the presented SVR training geometric algorithms have the same convergence and almost identical cost of computa-tion as their corresponding algorithms for SVM classification.Experimental results show that the geometric meth-ods are more efficient than conventional methods using quadratic programming and require much less memory.
