CaM BP-10 (BP-10) is anovel endogenous calmodalin (CaM )-binding Protein tha was observed in andisolated from Plant. In our prelidrimpstudies it has been shown tha BP-10specifically inhibits adrin (IAA)-in-duced coleo...CaM BP-10 (BP-10) is anovel endogenous calmodalin (CaM )-binding Protein tha was observed in andisolated from Plant. In our prelidrimpstudies it has been shown tha BP-10specifically inhibits adrin (IAA)-in-duced coleoptile elongallon and pbonsecrehon. In oIder to inveshgatewhether BP10 inhibitS Picton secrehonand cell elongaion thidgh inhibitingthc achvity of Plasrna mernbare (PM)H+ -ATPase, the effect of BP-10 on theachvity of wheat coleoptile PM H+ -AT-Pase has been studied. It was observedtha neither IAA nor BP-10 had any effect on the achvity of PM H+ -ATPaSein vitro. In vho, however, the activity0f Al-treated PM H+-ATPaSe washigher than tha of the control, and BP-10 peilicanx iwhbital atinduedachvity by 87- 8% (rig. 1) - In addi-hon, the inhibitory effect cotild be re-versed by IAA after BPIO was removedfrom the mediurn, and it could also beovercome bV the addihon of CaM.Therefore, it appears that BP-10 in-hibits cell responses to andn by affect-ing the achvity of PM H+ -ATPase andthereby inhibiting Proton secrehon. As aresult, cell elongaon is inhibited. AndCaM is also involved in this pmeess-展开更多
Glucagon is a polypeptide hormone of 29 amino acids which is synthesized by the α-cell of the islets of Langerhans of pancreas. One of its effects is to cause glycogenolysis and an increase in blood suger. In recent ...Glucagon is a polypeptide hormone of 29 amino acids which is synthesized by the α-cell of the islets of Langerhans of pancreas. One of its effects is to cause glycogenolysis and an increase in blood suger. In recent years it has been found that glucagon has several biological effects including the effect on contractility in cardiac muscle. Much attention has been drawn to this Subject. Since the strueture of glucagon is rather flexible, it is readily attcked by展开更多
Light induced phosphorylation of endogenous thylakoid membrane protein can be inhibited markedly by a novel inhibitor CaMBP 10 which is discovered and isolated from plant. The inhibitory effect of BP 10 can be elimina...Light induced phosphorylation of endogenous thylakoid membrane protein can be inhibited markedly by a novel inhibitor CaMBP 10 which is discovered and isolated from plant. The inhibitory effect of BP 10 can be eliminated by addition of CaM. At the same time, the phosphorylation can also be inhibited by EGTA or CaM antagonists , such as TFP (trifluoperazine) and W 7 (N (6 aminohexyl) 5 chloro 1 naphthalene sulfonamide). This result implies that (i) Ca 2+ and CaM most likely participate in and regulate plant photosynthesis; (ii) the kinase that catalyzes thylakoid membrane protein phosphorylation can be regulated by Ca 2+ and CaM. However, the further experiments indicate that BP 10 has no effect on dephosphorylation of thylakoid phosphoproteins .展开更多
文摘CaM BP-10 (BP-10) is anovel endogenous calmodalin (CaM )-binding Protein tha was observed in andisolated from Plant. In our prelidrimpstudies it has been shown tha BP-10specifically inhibits adrin (IAA)-in-duced coleoptile elongallon and pbonsecrehon. In oIder to inveshgatewhether BP10 inhibitS Picton secrehonand cell elongaion thidgh inhibitingthc achvity of Plasrna mernbare (PM)H+ -ATPase, the effect of BP-10 on theachvity of wheat coleoptile PM H+ -AT-Pase has been studied. It was observedtha neither IAA nor BP-10 had any effect on the achvity of PM H+ -ATPaSein vitro. In vho, however, the activity0f Al-treated PM H+-ATPaSe washigher than tha of the control, and BP-10 peilicanx iwhbital atinduedachvity by 87- 8% (rig. 1) - In addi-hon, the inhibitory effect cotild be re-versed by IAA after BPIO was removedfrom the mediurn, and it could also beovercome bV the addihon of CaM.Therefore, it appears that BP-10 in-hibits cell responses to andn by affect-ing the achvity of PM H+ -ATPase andthereby inhibiting Proton secrehon. As aresult, cell elongaon is inhibited. AndCaM is also involved in this pmeess-
文摘Glucagon is a polypeptide hormone of 29 amino acids which is synthesized by the α-cell of the islets of Langerhans of pancreas. One of its effects is to cause glycogenolysis and an increase in blood suger. In recent years it has been found that glucagon has several biological effects including the effect on contractility in cardiac muscle. Much attention has been drawn to this Subject. Since the strueture of glucagon is rather flexible, it is readily attcked by
文摘Light induced phosphorylation of endogenous thylakoid membrane protein can be inhibited markedly by a novel inhibitor CaMBP 10 which is discovered and isolated from plant. The inhibitory effect of BP 10 can be eliminated by addition of CaM. At the same time, the phosphorylation can also be inhibited by EGTA or CaM antagonists , such as TFP (trifluoperazine) and W 7 (N (6 aminohexyl) 5 chloro 1 naphthalene sulfonamide). This result implies that (i) Ca 2+ and CaM most likely participate in and regulate plant photosynthesis; (ii) the kinase that catalyzes thylakoid membrane protein phosphorylation can be regulated by Ca 2+ and CaM. However, the further experiments indicate that BP 10 has no effect on dephosphorylation of thylakoid phosphoproteins .
