The thermograms of the germination and growth of rice and tree seeds were determined and studied by using a newly constructed microcalorimeter build in this laboratory at Wuhan University. The thermograms show the exi...The thermograms of the germination and growth of rice and tree seeds were determined and studied by using a newly constructed microcalorimeter build in this laboratory at Wuhan University. The thermograms show the existence of physiological triphasic patterns (including imbibition,activation and growth stages)in the germination and growth process. Nonequilibrium thermodynamics of the germination and growth of seeds was considered under given conditions, and the thermodynamic functions of the germination and growth of seeds were calculated.展开更多
Thermodynamic and kinetic behaviour of the energy liberation of the mitochondria isolated from Guangchong 41 and Maxie fertile lines of rice during heat treatment were studied by using a Dupont 910 differential scanni...Thermodynamic and kinetic behaviour of the energy liberation of the mitochondria isolated from Guangchong 41 and Maxie fertile lines of rice during heat treatment were studied by using a Dupont 910 differential scanning calorimeter & 9900 Computer/Thermal analyzer sys-tem over he temperature range 273.12-353.12K. The DSC curves of the rice mitochondria were obtained. All the curves manifest themselves exothermic peaks, the exothermic peak curve are of the same type on the whole, but their thermodynamic values are different from each other. The exothermic peaks can be regarded as energy liberation peaks of the rice mitochondria in the heat-ing processes. The temperature variation of the thermodynamic values and the apparent kinetic parameters of the energy liberation of rice mitochondria were calculated. The results indicate that the energy liberation of the Guangchong 41 rice mitochondria is easier than that of the Maxie rice mitochondria, but the mechanism of the energy liberation of the Guangchong 41 rice mitochondria is more complicated than that, of the Maxie rice mitochondria in the heating process.展开更多
RT-PCR reactions, with template cDNA from etiolated seedlings of Yuetai maintainer lines (Oryzasativa L. ) and 2, 3 or 4 random primers, were carried out at anneal temperatures 36℃, 38℃, 40℃, 42℃ and45℃. Products...RT-PCR reactions, with template cDNA from etiolated seedlings of Yuetai maintainer lines (Oryzasativa L. ) and 2, 3 or 4 random primers, were carried out at anneal temperatures 36℃, 38℃, 40℃, 42℃ and45℃. Products were separated on 2% agarose gel. No products were obtained at both 42℃and 45℃. Most of themain bands obtained at 36℃, 38℃ and 40℃ were similar, but there are some differences in the weak bands. Thus,we select 40℃ as the anneal temperature in the following experiments. The difference of mRNAs, which were isolated from anthers at different developmental stages of Yuetai CMS. and maintainer lines, were displayed by agarosegel. Differential bands were retrieved and re-amplified. Then, products were purified on 3% agarose gel. Every purified band showed single band on 5% polyacrylamide gel.展开更多
文摘The thermograms of the germination and growth of rice and tree seeds were determined and studied by using a newly constructed microcalorimeter build in this laboratory at Wuhan University. The thermograms show the existence of physiological triphasic patterns (including imbibition,activation and growth stages)in the germination and growth process. Nonequilibrium thermodynamics of the germination and growth of seeds was considered under given conditions, and the thermodynamic functions of the germination and growth of seeds were calculated.
文摘Thermodynamic and kinetic behaviour of the energy liberation of the mitochondria isolated from Guangchong 41 and Maxie fertile lines of rice during heat treatment were studied by using a Dupont 910 differential scanning calorimeter & 9900 Computer/Thermal analyzer sys-tem over he temperature range 273.12-353.12K. The DSC curves of the rice mitochondria were obtained. All the curves manifest themselves exothermic peaks, the exothermic peak curve are of the same type on the whole, but their thermodynamic values are different from each other. The exothermic peaks can be regarded as energy liberation peaks of the rice mitochondria in the heat-ing processes. The temperature variation of the thermodynamic values and the apparent kinetic parameters of the energy liberation of rice mitochondria were calculated. The results indicate that the energy liberation of the Guangchong 41 rice mitochondria is easier than that of the Maxie rice mitochondria, but the mechanism of the energy liberation of the Guangchong 41 rice mitochondria is more complicated than that, of the Maxie rice mitochondria in the heating process.
文摘RT-PCR reactions, with template cDNA from etiolated seedlings of Yuetai maintainer lines (Oryzasativa L. ) and 2, 3 or 4 random primers, were carried out at anneal temperatures 36℃, 38℃, 40℃, 42℃ and45℃. Products were separated on 2% agarose gel. No products were obtained at both 42℃and 45℃. Most of themain bands obtained at 36℃, 38℃ and 40℃ were similar, but there are some differences in the weak bands. Thus,we select 40℃ as the anneal temperature in the following experiments. The difference of mRNAs, which were isolated from anthers at different developmental stages of Yuetai CMS. and maintainer lines, were displayed by agarosegel. Differential bands were retrieved and re-amplified. Then, products were purified on 3% agarose gel. Every purified band showed single band on 5% polyacrylamide gel.
