Using the crystal structure of Despentapeptide (B26-B30) insulin (DPI) as the search model, the crystal structure of DesBl-B2 Despentapeptide (B26-B30) insulin (DesBl-2 DPI) has been studied by the molecular replaceme...Using the crystal structure of Despentapeptide (B26-B30) insulin (DPI) as the search model, the crystal structure of DesBl-B2 Despentapeptide (B26-B30) insulin (DesBl-2 DPI) has been studied by the molecular replacement method. There is one DesBl-2 DPI molecule in each crystallographic asymmetric unit. The cross rotation function search and the translation function search show apparent peaks and thus determine the orientation and position of DesBl-2 DPI molecule in the cell respectively. The subsequent three-dimensional structural rebuilding and refine-ment of DesBl-2 DPI molecule confirm the results by molecular replacement method.展开更多
In order to explore the specific structure of the insulin analogues and the motion way of B-chain C-terminal peptide, Liang considered the amphipathic surface of insulin molecule to be the binding interaction surface ...In order to explore the specific structure of the insulin analogues and the motion way of B-chain C-terminal peptide, Liang considered the amphipathic surface of insulin molecule to be the binding interaction surface with its receptor and the conformational changes at B-chain C-terminus of insulin during the binding展开更多
基金Project supported by the Fonndation of Chinese Academy of Sciences and the National Natural Science Foundation of China
文摘Using the crystal structure of Despentapeptide (B26-B30) insulin (DPI) as the search model, the crystal structure of DesBl-B2 Despentapeptide (B26-B30) insulin (DesBl-2 DPI) has been studied by the molecular replacement method. There is one DesBl-2 DPI molecule in each crystallographic asymmetric unit. The cross rotation function search and the translation function search show apparent peaks and thus determine the orientation and position of DesBl-2 DPI molecule in the cell respectively. The subsequent three-dimensional structural rebuilding and refine-ment of DesBl-2 DPI molecule confirm the results by molecular replacement method.
基金Academia SinicaNational Natural Science Foundation of China.
文摘In order to explore the specific structure of the insulin analogues and the motion way of B-chain C-terminal peptide, Liang considered the amphipathic surface of insulin molecule to be the binding interaction surface with its receptor and the conformational changes at B-chain C-terminus of insulin during the binding
