钙调素(Calmodulin,CaM)是细胞内Ca2+信号的主要受体,能够与靶蛋白相互结合调节靶蛋白的活性,在细胞增殖、分化、凋亡、迁移等过程中都起着重要作用。荧光共振能量转移(f luorescence resonance energy transfer,FRET)技术是目前研究蛋...钙调素(Calmodulin,CaM)是细胞内Ca2+信号的主要受体,能够与靶蛋白相互结合调节靶蛋白的活性,在细胞增殖、分化、凋亡、迁移等过程中都起着重要作用。荧光共振能量转移(f luorescence resonance energy transfer,FRET)技术是目前研究蛋白质相互作用比较成熟的方法之一。作者通过Cre-loxP位点特异性重组技术构建了带有CFP荧光蛋白标记的文库,与YFP-CaM共同转染HEK293细胞,应用荧光共振能量转移技术(FRET)进行检测,挑取发生FRET作用的单个细胞,并进行单细胞PCR检测。由此扩增出的片段通过测序和蛋白序列数据库NCBI进行序列比对后,筛选出与CaM产生相互作用的蛋白。目前,已经通过这种方法成功地筛选到了一些与CaM相结合的蛋白,从而为进一步研究CaM蛋白在生理环境下的作用提供有利条件。展开更多
In this paper,we include the density dependence behavior of the symmetry energy in the improved quark mass density dependent (IQMDD) model.Under the mean field approximation,this model is applied to investigate neut...In this paper,we include the density dependence behavior of the symmetry energy in the improved quark mass density dependent (IQMDD) model.Under the mean field approximation,this model is applied to investigate neutron star matter and neutron stars successfully.Effects of the density dependence of the symmetry energy on neutron stars are described.展开更多
文摘钙调素(Calmodulin,CaM)是细胞内Ca2+信号的主要受体,能够与靶蛋白相互结合调节靶蛋白的活性,在细胞增殖、分化、凋亡、迁移等过程中都起着重要作用。荧光共振能量转移(f luorescence resonance energy transfer,FRET)技术是目前研究蛋白质相互作用比较成熟的方法之一。作者通过Cre-loxP位点特异性重组技术构建了带有CFP荧光蛋白标记的文库,与YFP-CaM共同转染HEK293细胞,应用荧光共振能量转移技术(FRET)进行检测,挑取发生FRET作用的单个细胞,并进行单细胞PCR检测。由此扩增出的片段通过测序和蛋白序列数据库NCBI进行序列比对后,筛选出与CaM产生相互作用的蛋白。目前,已经通过这种方法成功地筛选到了一些与CaM相结合的蛋白,从而为进一步研究CaM蛋白在生理环境下的作用提供有利条件。
基金Supported by National Natural Science Foundation of China (10535010, 10675090, 10775068, 10735010, 10975072)National Major State Basic Research and Development of China (2007CB815004)+1 种基金CAS Knowledge Innovation Project (KJCX2-SW-N02)Research Fund of Doctoral Point (RFDP) (200700284016)
文摘In this paper,we include the density dependence behavior of the symmetry energy in the improved quark mass density dependent (IQMDD) model.Under the mean field approximation,this model is applied to investigate neutron star matter and neutron stars successfully.Effects of the density dependence of the symmetry energy on neutron stars are described.