C-terminal sequencing is important for characterized the primary structure of protein and polypeptides and it must do for quality control of recombination protein drugs.A method of MALDI-TOF-TOF-MS with in source deca...C-terminal sequencing is important for characterized the primary structure of protein and polypeptides and it must do for quality control of recombination protein drugs.A method of MALDI-TOF-TOF-MS with in source decay(ISD) to measure C-terminal sequence of protein was established.This method was applied successfully to measure C-terminal sequence of Neuregulin,TP-Ⅱ,ubiquitin,and myoglobin,respectively and 10,24,24 and 36 amino acid residue of C-terminal were measured.展开更多
In order to explore the effect of electroacupuncture on rats with acute spinal injury,we established animal model of the spinal injury.Total proteins were extracted from spinal cord of normal rats,acute spinal injury ...In order to explore the effect of electroacupuncture on rats with acute spinal injury,we established animal model of the spinal injury.Total proteins were extracted from spinal cord of normal rats,acute spinal injury rats and acute spinal injury rat treated by electroacupuncture,and separated by 2-DE.10 differential expressed proteins were identified by the MALDI-TOF-TOF-MS with high mass accuracy,high mascot scores and high reliability.展开更多
文摘C-terminal sequencing is important for characterized the primary structure of protein and polypeptides and it must do for quality control of recombination protein drugs.A method of MALDI-TOF-TOF-MS with in source decay(ISD) to measure C-terminal sequence of protein was established.This method was applied successfully to measure C-terminal sequence of Neuregulin,TP-Ⅱ,ubiquitin,and myoglobin,respectively and 10,24,24 and 36 amino acid residue of C-terminal were measured.
文摘In order to explore the effect of electroacupuncture on rats with acute spinal injury,we established animal model of the spinal injury.Total proteins were extracted from spinal cord of normal rats,acute spinal injury rats and acute spinal injury rat treated by electroacupuncture,and separated by 2-DE.10 differential expressed proteins were identified by the MALDI-TOF-TOF-MS with high mass accuracy,high mascot scores and high reliability.
