Determination methods of trace Hg(Ⅱ),Cu(Ⅱ) and Cd(Ⅱ) were established based on the combination of LPME of dithizone-chelate for high enrichment,strip shape sampling-TLC and digital image-processing for quantitative...Determination methods of trace Hg(Ⅱ),Cu(Ⅱ) and Cd(Ⅱ) were established based on the combination of LPME of dithizone-chelate for high enrichment,strip shape sampling-TLC and digital image-processing for quantitative analysis.Detection limits of Cu(Ⅱ),Hg(Ⅱ) and Cd(Ⅱ) were 1.5 ng/mL,1.3 ng/mL and 2.5ng/mL,respectively.RSDs were 1.2%,4.0% and 2.7%,respectively.The proposed methods have the advantages of convenience,sensitiveness,precise and economicls.The methods were applied to the determination of these elements in a pool water and mud,with satisfying results.展开更多
凝胶电泳是常用的生化分离鉴定技术。生物分子大多在可见光区完全透明,因此在凝胶电泳之后,需要对凝胶进行染色处理才能观察到结果,而且常规的染色方法一般步骤比较繁琐耗时。本文开发了一种新型的预染色凝胶电泳方法(Pre-Staining Gel ...凝胶电泳是常用的生化分离鉴定技术。生物分子大多在可见光区完全透明,因此在凝胶电泳之后,需要对凝胶进行染色处理才能观察到结果,而且常规的染色方法一般步骤比较繁琐耗时。本文开发了一种新型的预染色凝胶电泳方法(Pre-Staining Gel Electrophoresis,PSGE),可以直观地显示电泳结果,并简化凝胶电泳操作。利用PSGE成功分析了蛋白质与两性高分子聚合物的共价偶联和物理吸附的效率,展示了PSGE的巨大应用开发潜力。展开更多
文摘Determination methods of trace Hg(Ⅱ),Cu(Ⅱ) and Cd(Ⅱ) were established based on the combination of LPME of dithizone-chelate for high enrichment,strip shape sampling-TLC and digital image-processing for quantitative analysis.Detection limits of Cu(Ⅱ),Hg(Ⅱ) and Cd(Ⅱ) were 1.5 ng/mL,1.3 ng/mL and 2.5ng/mL,respectively.RSDs were 1.2%,4.0% and 2.7%,respectively.The proposed methods have the advantages of convenience,sensitiveness,precise and economicls.The methods were applied to the determination of these elements in a pool water and mud,with satisfying results.
文摘凝胶电泳是常用的生化分离鉴定技术。生物分子大多在可见光区完全透明,因此在凝胶电泳之后,需要对凝胶进行染色处理才能观察到结果,而且常规的染色方法一般步骤比较繁琐耗时。本文开发了一种新型的预染色凝胶电泳方法(Pre-Staining Gel Electrophoresis,PSGE),可以直观地显示电泳结果,并简化凝胶电泳操作。利用PSGE成功分析了蛋白质与两性高分子聚合物的共价偶联和物理吸附的效率,展示了PSGE的巨大应用开发潜力。