戈登研讨会(Gordon Research Conference)是由美国John Hopkins University的NeilE.Gordon博士在19世纪20年代后期发起的。他看到当时不论是在相同领域或是在交叉学科的科学家之间建立直接的交流十分困难,于是倡导了戈登研讨会,目的在...戈登研讨会(Gordon Research Conference)是由美国John Hopkins University的NeilE.Gordon博士在19世纪20年代后期发起的。他看到当时不论是在相同领域或是在交叉学科的科学家之间建立直接的交流十分困难,于是倡导了戈登研讨会,目的在于促进科学家之间的联系和自由交换观点,以促进科学的发展。戈登研讨会是生物学、化学和物理学等学科前沿研究领域的专业性很强的小型会议。展开更多
Protein crystal growth is quite important for the determination of protein structureswhich are essential to the understanding of life at molecular level as well as to the development of molecu-lar biotechnology.The mi...Protein crystal growth is quite important for the determination of protein structureswhich are essential to the understanding of life at molecular level as well as to the development of molecu-lar biotechnology.The microgravity environment of space is an ideal place to study the complicated pro-tein crystallization and to grow good-quality protein crystals.A number of crystal-growth experiments of10 different proteins were carried out in August,1992 on the Chinese re-entry satellite FSW-2 in spaceusing a tube crystallization equipment made in China.A total of 25 samples from 6 proteins producedcrystals,and the effects of microgravity on protein crystal growth were observed,especially for an acidicphospholipase A2 and henegg-white lysozyme which gave better crystals in space than earth-grown crys-tals in ground control experiments.The results have shown that the microgravity in space favors the im-provement of the size,perfection,morphology and internal order of the grown protein crytals.展开更多
Rice dwarf virus (RDV) is a double-shelled icosahedral virus.Using electron cryomicroscopy and computer reconstruction techniques, we have determined a 3.3 nm resolution three-dimensional (3D) structure of the inner s...Rice dwarf virus (RDV) is a double-shelled icosahedral virus.Using electron cryomicroscopy and computer reconstruction techniques, we have determined a 3.3 nm resolution three-dimensional (3D) structure of the inner shell capsid without the outer shell and viral RNA. The results show that the inner shell is a thin, densely packed, smooth structure, which provides a scaffold for the full virus. A total of 120 copies of the major inner shell capsid protein P3 forms 60 dimers arranged in a T=1 icosahedral lattice. A close examination on the subunit packing of the T=1 inner core P3 with that of the T=13l outer shell P8 indicated that P8 trimers connect with P3 through completely non-equivalent, yet highly specific, intermolecular interactions.展开更多
The 3-dimensional structures of bovine chymotrypsin and bovine trypsin are one of the earliest fruitful achievements in the field of protein crystallography. In 1970, R. M. Stroud et al. disclosed the stereoscopic str...The 3-dimensional structures of bovine chymotrypsin and bovine trypsin are one of the earliest fruitful achievements in the field of protein crystallography. In 1970, R. M. Stroud et al. disclosed the stereoscopic structure of bovine trypsin at 2.7 (?) resolution. In 1972, J. J. Birktoft et al. also reported the 3-dimensional structure of bovine展开更多
文摘戈登研讨会(Gordon Research Conference)是由美国John Hopkins University的NeilE.Gordon博士在19世纪20年代后期发起的。他看到当时不论是在相同领域或是在交叉学科的科学家之间建立直接的交流十分困难,于是倡导了戈登研讨会,目的在于促进科学家之间的联系和自由交换观点,以促进科学的发展。戈登研讨会是生物学、化学和物理学等学科前沿研究领域的专业性很强的小型会议。
文摘Protein crystal growth is quite important for the determination of protein structureswhich are essential to the understanding of life at molecular level as well as to the development of molecu-lar biotechnology.The microgravity environment of space is an ideal place to study the complicated pro-tein crystallization and to grow good-quality protein crystals.A number of crystal-growth experiments of10 different proteins were carried out in August,1992 on the Chinese re-entry satellite FSW-2 in spaceusing a tube crystallization equipment made in China.A total of 25 samples from 6 proteins producedcrystals,and the effects of microgravity on protein crystal growth were observed,especially for an acidicphospholipase A2 and henegg-white lysozyme which gave better crystals in space than earth-grown crys-tals in ground control experiments.The results have shown that the microgravity in space favors the im-provement of the size,perfection,morphology and internal order of the grown protein crytals.
基金theNational Natural Science Foundation of China (Grant No. 39870181), NIH (USA, AI 46420 to ZHZ) and the Welch Foundation (AU-1492 to ZHZ).
文摘Rice dwarf virus (RDV) is a double-shelled icosahedral virus.Using electron cryomicroscopy and computer reconstruction techniques, we have determined a 3.3 nm resolution three-dimensional (3D) structure of the inner shell capsid without the outer shell and viral RNA. The results show that the inner shell is a thin, densely packed, smooth structure, which provides a scaffold for the full virus. A total of 120 copies of the major inner shell capsid protein P3 forms 60 dimers arranged in a T=1 icosahedral lattice. A close examination on the subunit packing of the T=1 inner core P3 with that of the T=13l outer shell P8 indicated that P8 trimers connect with P3 through completely non-equivalent, yet highly specific, intermolecular interactions.
文摘The 3-dimensional structures of bovine chymotrypsin and bovine trypsin are one of the earliest fruitful achievements in the field of protein crystallography. In 1970, R. M. Stroud et al. disclosed the stereoscopic structure of bovine trypsin at 2.7 (?) resolution. In 1972, J. J. Birktoft et al. also reported the 3-dimensional structure of bovine
