目的:观察麻荆止咳颗粒对咳嗽变异性哮喘(CVA)模型大鼠肺组织病理形态学变化及咳嗽次数的影响,探讨麻荆止咳颗粒改善CVA气道炎症的作用及疗效。方法:将60只SPF级SD雄性大鼠按照随机数字表法分为空白组、模型组、孟鲁司特钠组和麻荆止咳...目的:观察麻荆止咳颗粒对咳嗽变异性哮喘(CVA)模型大鼠肺组织病理形态学变化及咳嗽次数的影响,探讨麻荆止咳颗粒改善CVA气道炎症的作用及疗效。方法:将60只SPF级SD雄性大鼠按照随机数字表法分为空白组、模型组、孟鲁司特钠组和麻荆止咳颗粒低、中、高剂量组各10只。空白组不予造模,其余各组均采用卵清白蛋白(OVA)和氢氧化铝凝胶致敏并用OVA雾化激发的方法复制CVA模型大鼠。造模成功后,孟鲁司特钠组给予孟鲁司特钠溶液,麻荆止咳颗粒低、中、高剂量组分别给予麻荆止咳颗粒溶液对应药物剂量灌胃给药,空白组、模型组给予等体积蒸馏水,每天1次,连续14天。观察6组大鼠HE染色肺组织病理形态学变化及末次给药后3 min内咳嗽次数。结果:与空白组比较,模型组可见显著的气道炎症表现,末次给药后3 min咳嗽次数明显增多(P P P P > 0.05),麻荆止咳颗粒低、中、高剂量组气道炎症浸润改善情况无显著性差异(P > 0.05)。结论:麻荆止咳颗粒可减轻CVA大鼠肺组织的气道炎性损伤,减少咳嗽次数,从而减轻气道炎症,促进疾病恢复。Objective: To observe the effect of Majing Zhike Granules on the pathological morphological changes of lung tissue and the number of coughs in rats with cough variant asthma (CVA) model, and to explore the effect and efficacy of Majing Zhike Granules in improving CVA airway inflammation. Methods: Sixty SPF SD male rats were randomly divided into a blank group, a model group, a montelukast sodium group, and a low-, medium-, and high-dose Majing Zhike Granules group, 10 in each group. The blank group was not modeled, and the remaining groups were sensitized with ovalbumin (OVA) and aluminum hydroxide gel and stimulated with OVA aerosol to replicate the CVA model rats. After successful modeling, the montelukast sodium group was given montelukast sodium solution, and the low-, medium-, and high-dose Majing Zhike Granules groups were given Majing Zhike Granules solution by gavage at the corresponding drug doses. The blank group and model group were given equal volumes of distilled water once a day for 14 consecutive days. The pathological morphological changes of lung tissues stained with HE and the number of coughs within 3 minutes after the last administration of the drug were observed in the six groups of rats. Results: Compared with the blank group, the model group showed significant airway inflammation, and the number of coughs increased significantly 3 minutes after the last administration (P P P P > 0.05). There was no significant difference in the improvement of airway inflammatory infiltration in the low, medium and high dose groups of Majing Zhike Granules (P > 0.05). Conclusion: Majing Zhike Granules can reduce airway inflammatory damage in the lung tissue of CVA rats, reduce the number of coughs, thereby reducing airway inflammation and promoting disease recovery.展开更多
目的:观察芩夏清热祛风颗粒对感染后咳嗽(PIC)模型大鼠血清P物质(SP)、神经激肽A (NKA)、神经激肽B (NKB)、降钙素基因相关肽(CGRP)含量及肺组织SP、NKA、NKB、CGRP蛋白表达水平的影响,探讨芩夏清热祛风颗粒改善PIC气道神经源性炎症的...目的:观察芩夏清热祛风颗粒对感染后咳嗽(PIC)模型大鼠血清P物质(SP)、神经激肽A (NKA)、神经激肽B (NKB)、降钙素基因相关肽(CGRP)含量及肺组织SP、NKA、NKB、CGRP蛋白表达水平的影响,探讨芩夏清热祛风颗粒改善PIC气道神经源性炎症的作用机制。方法:将60只SPF级SD雄性大鼠按照随机数字表法分为空白组、模型组、对照组和低、中、高剂量组各10只。空白组不予造模,其余各组均采用烟熏结合脂多糖(LPS)溶液滴鼻及辣椒素雾化吸入制备感染后咳嗽大鼠模型。造模成功后,对照组按体质量4.78 ml/(kg∙d)给予复方甲氧那明溶液灌胃,低、中、高剂量组分别按体质量44.2 ml/(kg∙d)、93.6 ml/(kg∙d)、127.8 ml/(kg∙d)给予芩夏清热祛风颗粒溶液灌胃,空白组、模型组按体质量10 mL/(kg∙d) 0.9% NaCl灌胃给药,每天1次,连续14 d,空白组不做任何处理。观察6组大鼠末次给药后3min内咳嗽次数,HE染色观察6组大鼠肺组织病理形态学变化,ELISA法检测6组大鼠血清SP、NKA、NKB、CGRP含量,免疫组化法检测6组大鼠肺组织SP、NKA、NKB、CGRP蛋白表达。结果:与空白组比较,模型组末次给药后3min咳嗽次数明显增多(P 0.05);与对照组比较,中、高剂量组血清SP、NKA、NKB、CGRP含量和肺组织SP、NKA、NKB、CGRP蛋白表达水平无显著性差异(P > 0.05),低剂量组血清SP、NKA、NKB、CGRP含量和肺组织SP、NKA、NKB、CGRP蛋白表达水平较高(P Objective: To observe the effects of Qinxia Qingre Qufeng Granules on the levels of serum substance P (SP), neurokinin A (NKA), neurokinin B (NKB), and calcitonin gene-related peptide (CGRP) and the expression levels of SP, NKA, NKB, and CGRP proteins in lung tissues in rats with post-infectious cough (PIC) model, and to explore the mechanism of action of Qinxia Qingre Qufeng Granules in improving airway neurogenic inflammation in PIC. Methods: Sixty SPF SD male rats were randomly divided into blank group, model group, control group, and low, medium, and high dose groups, with 10 rats in each group. No model was established in the blank group, and the rat model of post-infectious cough was established in the other groups by smoke combined with lipopolysaccharide (LPS) solution nasal drops and capsaicin atomization inhalation. After successful modeling, the control group was given compound methoxyphenamine solution by gavage at 4.78 ml/(kg·d) of body weight, the low, medium and high dose groups were given Qinxia Qingre Qufeng granule solution by gavage at 44.2 ml/(kg·d), 93.6 ml/(kg·d) and 127.8 ml/(kg·d) of body weight, respectively, the blank group and model group were given 0.9% NaCl by gavage at 10 mL/(kg·d) of body weight, once a day for 14 consecutive days, and the blank group did not receive any treatment. The number of coughs within 3 minutes after the last administration was observed in the six groups of rats, the pathological morphological changes of the lung tissues of the six groups of rats were observed by HE staining, the serum SP, NKA, NKB and CGRP levels of the six groups of rats were detected by ELISA, and the SP, NKA, NKB and CGRP protein expressions of the lung tissues of the six groups of rats were detected by immunohistochemistry. Results: Compared with the blank group, the number of coughs in the model group increased significantly 3 minutes after the last administration (P 0.05). Compared with the control group, there were no significant differences in the serum SP, NKA, NKB, and CGRP levels and the protein expression levels of SP, NKA, NKB, and CGRP in lung tissues of the medium- and high-dose groups (P > 0.05), and the serum SP, NKA, NKB, and CGRP levels and the protein expression levels of SP, NKA, NKB, and CGRP in lung tissues of the low-dose group were higher (P < 0.05). Conclusion: Qinxia Qingre Qufeng Granule can reduce the serum SP, NKA, NKB, and CGRP levels of PIC rats, inhibit the protein expression of SP, NKA, NKB, and CGRP in rat lung tissues, and reduce the inflammatory infiltration of lung tissues, thereby reducing airway neurogenic inflammation and promoting disease recovery.展开更多
In our previous work, acupuncture induced inhibition of the nociceptive respons e to peripheral noxious stimulation at spinal level could be blocked by iontopho retic bicuculline(Bic), an antagonist for GABA A recepto...In our previous work, acupuncture induced inhibition of the nociceptive respons e to peripheral noxious stimulation at spinal level could be blocked by iontopho retic bicuculline(Bic), an antagonist for GABA A receptors, suggesting an invol vement of GABA in acupuncture induced segmental inhibition. However some report s declare that increase in GABA content in brain is not benefit to acupuncture ana lgesia. In this paper, the effects of intra cerebroventricular and intrathecal i njection (icv and ith) of Bic on acupuncture analgesia were further investigated by tail flick latency tests in rats. There were 6～8 rats in each group. Th e results were as follows: 1. After icv GABA in dose of 0, 125, 250, 500 and 1000 μg /5 μL, the pain thre shold (PT) were raised to 102%, 108%, 128%, 136%, and 157% of the basal control value respectively. It indic ates that icv GABA could produce dose dependent analgesic effect. 2. After icv Bic at the dose of 10 and 20 μg /5 μL, icv GABA (500 μg /5 μL) induced analgesic effect lowered from 136.24±1.96% to 111.8±0.98% and 111.25±0.65% separately. It means that icv GABA induced analgesic effect is significantly blocked by preinjection of Bic. 3. After electro acupuncture at bilateral "Ciliao" (BL 32) points (50 Hz, 1～2 mA) for 10 minutes, PT were raised to 142.50±2.10% without any treatment and 1 4 3.72±2.04% with pretreatment of saline. When pretreated with icv Bic at doses o f 10 and 20 μg /5 μL, acupuncture analgesic effects (PT still raised to 141.74 ±1.54% and 146.71±1.85%) showed no significant reduction. It indicates tha t GABA in brain might not be involved in acupuncture analgesia. 4. After electro acupuncture at bilateral "Ciliao"(BL 32), PT were raised to 139.56±1.21% with ith saline and to 138.96±1.43% pretreated with ith Bic 5 μg /10 μL. When pretreated with ith Bic at doses of 10 and 20 μg /10 μL, PT wer e raised to 126.55±1.73% and 114.52±1.68% respectively, indicating the signifi cant reduction of acupuncture analgesia. It means that GABA might be involved in acu puncture analgesia mediated by activation of A receptors at spinal level.展开更多
In our previous work, acupuncture induced elevation of pain threshold could not be blocked by intra cerebroventricular injection of bicuculline, an antagonist f or GABA A receptors, suggesting that GABA in brain might...In our previous work, acupuncture induced elevation of pain threshold could not be blocked by intra cerebroventricular injection of bicuculline, an antagonist f or GABA A receptors, suggesting that GABA in brain might not be involved in acup uncture analgesia, at least might not be mediated by activation of GABA A recept ors. In this paper, the effects of intra cerebroventricular and intrathecal inj e ction (icv and ith) of CGP 55845, a potent and selective antagonist against GABA B receptor, on acupuncture analgesia were further investigated by tail flick la tency tests in rats. There were 6～8 rats in each group. The results were as f ollows: 1. After icv and ith baclofen(Bac), an agonist of GABA B receptor at doses of 0. 0 25 μg /5 μL and 0.25 μg /10 μL(+5 μL saline) , pain threshold (PT) were rai sed to 125.11±1.94% and 128.63± 0.93% of the basal control value respective ly. While subcutaneous injection of Bac, 50 μg /kg was needed to produce significant analgesic effect (PT raised to 130.94±1.62%). It indicates that activation of GABA B receptor co uld produce analgesic effects mainly in the central nervous system. 2. After icv CGP 55845 at doses of 0.5, 5.0, and 50 ng /5 μL, icv Bac (2.5 μg /5 μL) induced analgesic effect (PT raised to 136.24±1.96%) was blocked by 3 7.4%, 77.0% and 75.1% respectively (PT raised to 130.43±1.72%, 111.8±0.98% and 111.25±0.65%). It means that icv Bac induced analgesic effect could be signif icantly blocked by pretreatment of icv CGP 55845. 3. After electroacupuncture at bilateral "Ciliao"(BL 32) points (50 Hz, 1～2 mA) for 10 minutes, PT were raised to 142.50±2.10 % without treatment, 143.74±2.0 4% with pretreatment of saline and 142.47±1.18% with pre icv CGP 55845 at dose s of 0.5 ng/5 μL. When pretreated with icv CGP 55845 at doses of 5 and 50 ng/ 5 μL, acupuncture analgesic effects were significantly blocked by 73.7% and 71. 6% (PT raised to 111.19±1.20% and 112.09±1.12%). It indicates that GABA in brai n might be involved in acupuncture analgesia mainly mediated by activation of GA BA B receptors. 4. After electroacupuncture at bilateral "Ciliao"(BL 32) points, PT were ra ised to 138.15± 1.64% with pretreatment(ith) of saline and to 138.56±1 .21% with pretreatment(ith) of CGP 55845 5 ng/5 μL. When pretreated with ith CGP 55 845 at doses of 50 and 500 ng /5 μL, PT were raised to 119.04±1.04% and 109.08 ±1.94% respectively, indicating that acupuncture analgesia could be significant ly blocked by 52.6% and 76.7%. It means that GABA might also be involved in acup uncture analgesia mediated by activation of B receptors at spinal level.展开更多
文摘目的:观察麻荆止咳颗粒对咳嗽变异性哮喘(CVA)模型大鼠肺组织病理形态学变化及咳嗽次数的影响,探讨麻荆止咳颗粒改善CVA气道炎症的作用及疗效。方法:将60只SPF级SD雄性大鼠按照随机数字表法分为空白组、模型组、孟鲁司特钠组和麻荆止咳颗粒低、中、高剂量组各10只。空白组不予造模,其余各组均采用卵清白蛋白(OVA)和氢氧化铝凝胶致敏并用OVA雾化激发的方法复制CVA模型大鼠。造模成功后,孟鲁司特钠组给予孟鲁司特钠溶液,麻荆止咳颗粒低、中、高剂量组分别给予麻荆止咳颗粒溶液对应药物剂量灌胃给药,空白组、模型组给予等体积蒸馏水,每天1次,连续14天。观察6组大鼠HE染色肺组织病理形态学变化及末次给药后3 min内咳嗽次数。结果:与空白组比较,模型组可见显著的气道炎症表现,末次给药后3 min咳嗽次数明显增多(P P P P > 0.05),麻荆止咳颗粒低、中、高剂量组气道炎症浸润改善情况无显著性差异(P > 0.05)。结论:麻荆止咳颗粒可减轻CVA大鼠肺组织的气道炎性损伤,减少咳嗽次数,从而减轻气道炎症,促进疾病恢复。Objective: To observe the effect of Majing Zhike Granules on the pathological morphological changes of lung tissue and the number of coughs in rats with cough variant asthma (CVA) model, and to explore the effect and efficacy of Majing Zhike Granules in improving CVA airway inflammation. Methods: Sixty SPF SD male rats were randomly divided into a blank group, a model group, a montelukast sodium group, and a low-, medium-, and high-dose Majing Zhike Granules group, 10 in each group. The blank group was not modeled, and the remaining groups were sensitized with ovalbumin (OVA) and aluminum hydroxide gel and stimulated with OVA aerosol to replicate the CVA model rats. After successful modeling, the montelukast sodium group was given montelukast sodium solution, and the low-, medium-, and high-dose Majing Zhike Granules groups were given Majing Zhike Granules solution by gavage at the corresponding drug doses. The blank group and model group were given equal volumes of distilled water once a day for 14 consecutive days. The pathological morphological changes of lung tissues stained with HE and the number of coughs within 3 minutes after the last administration of the drug were observed in the six groups of rats. Results: Compared with the blank group, the model group showed significant airway inflammation, and the number of coughs increased significantly 3 minutes after the last administration (P P P P > 0.05). There was no significant difference in the improvement of airway inflammatory infiltration in the low, medium and high dose groups of Majing Zhike Granules (P > 0.05). Conclusion: Majing Zhike Granules can reduce airway inflammatory damage in the lung tissue of CVA rats, reduce the number of coughs, thereby reducing airway inflammation and promoting disease recovery.
文摘目的:观察芩夏清热祛风颗粒对感染后咳嗽(PIC)模型大鼠血清P物质(SP)、神经激肽A (NKA)、神经激肽B (NKB)、降钙素基因相关肽(CGRP)含量及肺组织SP、NKA、NKB、CGRP蛋白表达水平的影响,探讨芩夏清热祛风颗粒改善PIC气道神经源性炎症的作用机制。方法:将60只SPF级SD雄性大鼠按照随机数字表法分为空白组、模型组、对照组和低、中、高剂量组各10只。空白组不予造模,其余各组均采用烟熏结合脂多糖(LPS)溶液滴鼻及辣椒素雾化吸入制备感染后咳嗽大鼠模型。造模成功后,对照组按体质量4.78 ml/(kg∙d)给予复方甲氧那明溶液灌胃,低、中、高剂量组分别按体质量44.2 ml/(kg∙d)、93.6 ml/(kg∙d)、127.8 ml/(kg∙d)给予芩夏清热祛风颗粒溶液灌胃,空白组、模型组按体质量10 mL/(kg∙d) 0.9% NaCl灌胃给药,每天1次,连续14 d,空白组不做任何处理。观察6组大鼠末次给药后3min内咳嗽次数,HE染色观察6组大鼠肺组织病理形态学变化,ELISA法检测6组大鼠血清SP、NKA、NKB、CGRP含量,免疫组化法检测6组大鼠肺组织SP、NKA、NKB、CGRP蛋白表达。结果:与空白组比较,模型组末次给药后3min咳嗽次数明显增多(P 0.05);与对照组比较,中、高剂量组血清SP、NKA、NKB、CGRP含量和肺组织SP、NKA、NKB、CGRP蛋白表达水平无显著性差异(P > 0.05),低剂量组血清SP、NKA、NKB、CGRP含量和肺组织SP、NKA、NKB、CGRP蛋白表达水平较高(P Objective: To observe the effects of Qinxia Qingre Qufeng Granules on the levels of serum substance P (SP), neurokinin A (NKA), neurokinin B (NKB), and calcitonin gene-related peptide (CGRP) and the expression levels of SP, NKA, NKB, and CGRP proteins in lung tissues in rats with post-infectious cough (PIC) model, and to explore the mechanism of action of Qinxia Qingre Qufeng Granules in improving airway neurogenic inflammation in PIC. Methods: Sixty SPF SD male rats were randomly divided into blank group, model group, control group, and low, medium, and high dose groups, with 10 rats in each group. No model was established in the blank group, and the rat model of post-infectious cough was established in the other groups by smoke combined with lipopolysaccharide (LPS) solution nasal drops and capsaicin atomization inhalation. After successful modeling, the control group was given compound methoxyphenamine solution by gavage at 4.78 ml/(kg·d) of body weight, the low, medium and high dose groups were given Qinxia Qingre Qufeng granule solution by gavage at 44.2 ml/(kg·d), 93.6 ml/(kg·d) and 127.8 ml/(kg·d) of body weight, respectively, the blank group and model group were given 0.9% NaCl by gavage at 10 mL/(kg·d) of body weight, once a day for 14 consecutive days, and the blank group did not receive any treatment. The number of coughs within 3 minutes after the last administration was observed in the six groups of rats, the pathological morphological changes of the lung tissues of the six groups of rats were observed by HE staining, the serum SP, NKA, NKB and CGRP levels of the six groups of rats were detected by ELISA, and the SP, NKA, NKB and CGRP protein expressions of the lung tissues of the six groups of rats were detected by immunohistochemistry. Results: Compared with the blank group, the number of coughs in the model group increased significantly 3 minutes after the last administration (P 0.05). Compared with the control group, there were no significant differences in the serum SP, NKA, NKB, and CGRP levels and the protein expression levels of SP, NKA, NKB, and CGRP in lung tissues of the medium- and high-dose groups (P > 0.05), and the serum SP, NKA, NKB, and CGRP levels and the protein expression levels of SP, NKA, NKB, and CGRP in lung tissues of the low-dose group were higher (P < 0.05). Conclusion: Qinxia Qingre Qufeng Granule can reduce the serum SP, NKA, NKB, and CGRP levels of PIC rats, inhibit the protein expression of SP, NKA, NKB, and CGRP in rat lung tissues, and reduce the inflammatory infiltration of lung tissues, thereby reducing airway neurogenic inflammation and promoting disease recovery.
文摘In our previous work, acupuncture induced inhibition of the nociceptive respons e to peripheral noxious stimulation at spinal level could be blocked by iontopho retic bicuculline(Bic), an antagonist for GABA A receptors, suggesting an invol vement of GABA in acupuncture induced segmental inhibition. However some report s declare that increase in GABA content in brain is not benefit to acupuncture ana lgesia. In this paper, the effects of intra cerebroventricular and intrathecal i njection (icv and ith) of Bic on acupuncture analgesia were further investigated by tail flick latency tests in rats. There were 6～8 rats in each group. Th e results were as follows: 1. After icv GABA in dose of 0, 125, 250, 500 and 1000 μg /5 μL, the pain thre shold (PT) were raised to 102%, 108%, 128%, 136%, and 157% of the basal control value respectively. It indic ates that icv GABA could produce dose dependent analgesic effect. 2. After icv Bic at the dose of 10 and 20 μg /5 μL, icv GABA (500 μg /5 μL) induced analgesic effect lowered from 136.24±1.96% to 111.8±0.98% and 111.25±0.65% separately. It means that icv GABA induced analgesic effect is significantly blocked by preinjection of Bic. 3. After electro acupuncture at bilateral "Ciliao" (BL 32) points (50 Hz, 1～2 mA) for 10 minutes, PT were raised to 142.50±2.10% without any treatment and 1 4 3.72±2.04% with pretreatment of saline. When pretreated with icv Bic at doses o f 10 and 20 μg /5 μL, acupuncture analgesic effects (PT still raised to 141.74 ±1.54% and 146.71±1.85%) showed no significant reduction. It indicates tha t GABA in brain might not be involved in acupuncture analgesia. 4. After electro acupuncture at bilateral "Ciliao"(BL 32), PT were raised to 139.56±1.21% with ith saline and to 138.96±1.43% pretreated with ith Bic 5 μg /10 μL. When pretreated with ith Bic at doses of 10 and 20 μg /10 μL, PT wer e raised to 126.55±1.73% and 114.52±1.68% respectively, indicating the signifi cant reduction of acupuncture analgesia. It means that GABA might be involved in acu puncture analgesia mediated by activation of A receptors at spinal level.
文摘In our previous work, acupuncture induced elevation of pain threshold could not be blocked by intra cerebroventricular injection of bicuculline, an antagonist f or GABA A receptors, suggesting that GABA in brain might not be involved in acup uncture analgesia, at least might not be mediated by activation of GABA A recept ors. In this paper, the effects of intra cerebroventricular and intrathecal inj e ction (icv and ith) of CGP 55845, a potent and selective antagonist against GABA B receptor, on acupuncture analgesia were further investigated by tail flick la tency tests in rats. There were 6～8 rats in each group. The results were as f ollows: 1. After icv and ith baclofen(Bac), an agonist of GABA B receptor at doses of 0. 0 25 μg /5 μL and 0.25 μg /10 μL(+5 μL saline) , pain threshold (PT) were rai sed to 125.11±1.94% and 128.63± 0.93% of the basal control value respective ly. While subcutaneous injection of Bac, 50 μg /kg was needed to produce significant analgesic effect (PT raised to 130.94±1.62%). It indicates that activation of GABA B receptor co uld produce analgesic effects mainly in the central nervous system. 2. After icv CGP 55845 at doses of 0.5, 5.0, and 50 ng /5 μL, icv Bac (2.5 μg /5 μL) induced analgesic effect (PT raised to 136.24±1.96%) was blocked by 3 7.4%, 77.0% and 75.1% respectively (PT raised to 130.43±1.72%, 111.8±0.98% and 111.25±0.65%). It means that icv Bac induced analgesic effect could be signif icantly blocked by pretreatment of icv CGP 55845. 3. After electroacupuncture at bilateral "Ciliao"(BL 32) points (50 Hz, 1～2 mA) for 10 minutes, PT were raised to 142.50±2.10 % without treatment, 143.74±2.0 4% with pretreatment of saline and 142.47±1.18% with pre icv CGP 55845 at dose s of 0.5 ng/5 μL. When pretreated with icv CGP 55845 at doses of 5 and 50 ng/ 5 μL, acupuncture analgesic effects were significantly blocked by 73.7% and 71. 6% (PT raised to 111.19±1.20% and 112.09±1.12%). It indicates that GABA in brai n might be involved in acupuncture analgesia mainly mediated by activation of GA BA B receptors. 4. After electroacupuncture at bilateral "Ciliao"(BL 32) points, PT were ra ised to 138.15± 1.64% with pretreatment(ith) of saline and to 138.56±1 .21% with pretreatment(ith) of CGP 55845 5 ng/5 μL. When pretreated with ith CGP 55 845 at doses of 50 and 500 ng /5 μL, PT were raised to 119.04±1.04% and 109.08 ±1.94% respectively, indicating that acupuncture analgesia could be significant ly blocked by 52.6% and 76.7%. It means that GABA might also be involved in acup uncture analgesia mediated by activation of B receptors at spinal level.
