单链克隆DNA的反向测序是一个简单和快速的方法,这个方法可利用原来的DNA再进行反向顺序测定,从而达到校正和积累DNA数据的作用。由于反向测序的本质是双链测序,所得到的DNA顺序的图谱背景较深,伴有杂带,或在X光显影上是不清晰的。本文...单链克隆DNA的反向测序是一个简单和快速的方法,这个方法可利用原来的DNA再进行反向顺序测定,从而达到校正和积累DNA数据的作用。由于反向测序的本质是双链测序,所得到的DNA顺序的图谱背景较深,伴有杂带,或在X光显影上是不清晰的。本文报道利用我们实验室所开发的热稳定性的Bst聚合酶系统,进行反向测序克服了这些缺点,获得的图谱可以和单链DNA模板测序的图谱相媲美。我们以nod D 487-mp 8 ss-DNA为模板进行反向测序,证明Bst聚合酶在65℃反应能得到满意的结果。展开更多
In Rhizobium leguminosarum (R.1.) biovar viciae, the nodulation (nod) gene D encodes a transcriptional activator (NodD). It also represses nodD transcription,and this autoregulation occurs at the divergent and overlap...In Rhizobium leguminosarum (R.1.) biovar viciae, the nodulation (nod) gene D encodes a transcriptional activator (NodD). It also represses nodD transcription,and this autoregulation occurs at the divergent and overlapping nodA-nodD promoters.展开更多
Scientists have long been interested in the synthesis and medical use of 4’-thionudeosides. However, their effect on DNA synthesis has not been well studied. In order to study their bioactivity and explore new applic...Scientists have long been interested in the synthesis and medical use of 4’-thionudeosides. However, their effect on DNA synthesis has not been well studied. In order to study their bioactivity and explore new applications, we have synthesized 4’-thio-2’-deoxythymidine-5’-triphosphate (T’TP). The experimental results obtained in our lab showed that T’TP is a strong inhibitor of DNA polymerase, and the inhibition is highly specific. These prbperties indicate the potential of T’TP used for antitumor and antiviral agents as well as for the terminator in DNA sequencing.展开更多
文摘单链克隆DNA的反向测序是一个简单和快速的方法,这个方法可利用原来的DNA再进行反向顺序测定,从而达到校正和积累DNA数据的作用。由于反向测序的本质是双链测序,所得到的DNA顺序的图谱背景较深,伴有杂带,或在X光显影上是不清晰的。本文报道利用我们实验室所开发的热稳定性的Bst聚合酶系统,进行反向测序克服了这些缺点,获得的图谱可以和单链DNA模板测序的图谱相媲美。我们以nod D 487-mp 8 ss-DNA为模板进行反向测序,证明Bst聚合酶在65℃反应能得到满意的结果。
文摘In Rhizobium leguminosarum (R.1.) biovar viciae, the nodulation (nod) gene D encodes a transcriptional activator (NodD). It also represses nodD transcription,and this autoregulation occurs at the divergent and overlapping nodA-nodD promoters.
文摘Scientists have long been interested in the synthesis and medical use of 4’-thionudeosides. However, their effect on DNA synthesis has not been well studied. In order to study their bioactivity and explore new applications, we have synthesized 4’-thio-2’-deoxythymidine-5’-triphosphate (T’TP). The experimental results obtained in our lab showed that T’TP is a strong inhibitor of DNA polymerase, and the inhibition is highly specific. These prbperties indicate the potential of T’TP used for antitumor and antiviral agents as well as for the terminator in DNA sequencing.
