建立了蔬菜中4种喹诺酮类抗生素的超高效液相色谱-电喷雾串联质谱(UPLC-ESI-MS/MS)分析方法。每克蔬菜样品(干重)以15 mL乙腈-HCl(125∶8,V/V)进行振荡-超声提取3次,用HLB固相萃取柱进行净化富集,以6 mL 1%酸化乙腈进行洗脱,用N2进行吹...建立了蔬菜中4种喹诺酮类抗生素的超高效液相色谱-电喷雾串联质谱(UPLC-ESI-MS/MS)分析方法。每克蔬菜样品(干重)以15 mL乙腈-HCl(125∶8,V/V)进行振荡-超声提取3次,用HLB固相萃取柱进行净化富集,以6 mL 1%酸化乙腈进行洗脱,用N2进行吹脱,最后用初始流动相进行定容。以0.1%甲酸-乙腈溶液和0.1%甲酸溶液作为流动相,采用梯度洗脱方式进行UPLC-ESI-MS/MS检测。蔬菜中4种喹诺酮类化合物不同浓度加标回收率为61%~90%;相对标准偏差(RSD)小于5%(个别除外);检出限为0.021~0.092μg/kg;定量限为0.065~0.312μg/kg。本方法能够满足实际蔬菜样品的分析要求。展开更多
Jade carving is a form of sculpting, which is valued as a high-class form of art for both appreciation and decoration. Given the value of the jade and the skill required to make carvings, jade carvings have tremendous...Jade carving is a form of sculpting, which is valued as a high-class form of art for both appreciation and decoration. Given the value of the jade and the skill required to make carvings, jade carvings have tremendous economic value.展开更多
文摘建立了蔬菜中4种喹诺酮类抗生素的超高效液相色谱-电喷雾串联质谱(UPLC-ESI-MS/MS)分析方法。每克蔬菜样品(干重)以15 mL乙腈-HCl(125∶8,V/V)进行振荡-超声提取3次,用HLB固相萃取柱进行净化富集,以6 mL 1%酸化乙腈进行洗脱,用N2进行吹脱,最后用初始流动相进行定容。以0.1%甲酸-乙腈溶液和0.1%甲酸溶液作为流动相,采用梯度洗脱方式进行UPLC-ESI-MS/MS检测。蔬菜中4种喹诺酮类化合物不同浓度加标回收率为61%~90%;相对标准偏差(RSD)小于5%(个别除外);检出限为0.021~0.092μg/kg;定量限为0.065~0.312μg/kg。本方法能够满足实际蔬菜样品的分析要求。
文摘Jade carving is a form of sculpting, which is valued as a high-class form of art for both appreciation and decoration. Given the value of the jade and the skill required to make carvings, jade carvings have tremendous economic value.