During the screening of a traditional Chinese folk herb library against Hep G2 and Hep3 B cell lines, the Et OAc extract from the Tibetan medicine, Caragana tibetica（CT-Et OAc） exhibited potential anti-hepatocellula...During the screening of a traditional Chinese folk herb library against Hep G2 and Hep3 B cell lines, the Et OAc extract from the Tibetan medicine, Caragana tibetica（CT-Et OAc） exhibited potential anti-hepatocellular carcinoma（anti-HCC） activity. HPLC-based activity profiling was performed for targeted identification of anti-HCC activity from CT-Et OAc by MS-directed purification method. CT-Et OAc was separated by time-based fractionation for further anti-HCC bioassay by a semipreparative HPLC column（150 mm × 10 mm i.d., 5 μm） with a single injection of 5 mg. Bioassay-guided and ESIMS-directed large scale purification was performed with a single injection of 400 mg of CT-Et OAc by peak-based fractionation. A 1.4-mm heavy wall micro NMR tube with z-gradient was used to measure one and two dimensional NMR spectra for the minor or trace amounts of components of the extract. Two active compounds could be elucidated as naringenin chalcone（CT-1） and 3-hydroxy-8, 9-dimethoxypterocarpan（CT-2） relevant to anti-HCC effects for the Et OAc extract of C. tibetica rapidly and unambiguously by this protocol.展开更多
文摘从斑蝥(Lytta vesicatoria)体内分离得一株具有显著抗肝癌活性的真菌菌株,通过菌株形态与18S r DNA序列分析,鉴定为尖孢镰刀菌(Fusarium oxysporum,BM2).运用中压柱色谱和制备高效制备液相方法对BM2发酵液提取物乙酸乙酯部位的化学成分进行了分离纯化,利用现代波谱技术鉴定所分离化合物的结构,并采用MTT法对所得到的化合物进行了体外抗肝癌活性测试.结果表明:从发酵液提取物乙酸乙酯部位中分离得到的6个单体化合物,分别鉴定为麦角甾醇(1)、cyclo(R-Pro-S-Phe)(2)、(R)-2-羟基-3-苯基丙酸甲酯(3),4-氧代-乙酰丙酸(4),N-(4-氧代戊基)-乙酰胺(5),(R)-2-羟基-3-(4'-羟基苯基)丙酸甲酯(6).化合物2对2个肝癌细胞株Hep G2和SMMC-7721均有抑制作用,其IC50值分别为44.1和47.6μg/m L.
基金supported by grants from Natural Science Foundation of Qinghai Province(No.2012-z-904)the Major Research Project of Department of Education of Hubei Province,China(No.D20131103)+1 种基金National Natural Science Foundation of China(No.81102798)Wuhan Youth Chenguang Program of Science and Technology(No.2013070104010028)
文摘During the screening of a traditional Chinese folk herb library against Hep G2 and Hep3 B cell lines, the Et OAc extract from the Tibetan medicine, Caragana tibetica（CT-Et OAc） exhibited potential anti-hepatocellular carcinoma（anti-HCC） activity. HPLC-based activity profiling was performed for targeted identification of anti-HCC activity from CT-Et OAc by MS-directed purification method. CT-Et OAc was separated by time-based fractionation for further anti-HCC bioassay by a semipreparative HPLC column（150 mm × 10 mm i.d., 5 μm） with a single injection of 5 mg. Bioassay-guided and ESIMS-directed large scale purification was performed with a single injection of 400 mg of CT-Et OAc by peak-based fractionation. A 1.4-mm heavy wall micro NMR tube with z-gradient was used to measure one and two dimensional NMR spectra for the minor or trace amounts of components of the extract. Two active compounds could be elucidated as naringenin chalcone（CT-1） and 3-hydroxy-8, 9-dimethoxypterocarpan（CT-2） relevant to anti-HCC effects for the Et OAc extract of C. tibetica rapidly and unambiguously by this protocol.
