目的探讨端粒酶抑制因子PinX1(Pin2/TRF1-interacting protein X1)在宫颈癌细胞系、正常宫颈组织标本、宫颈癌组织标本中的变异情况。方法采用3种宫颈癌细胞系(加以3种淋巴瘤细胞系、1种乳腺癌细胞系作为参照)、28例正常宫颈组织及52例...目的探讨端粒酶抑制因子PinX1(Pin2/TRF1-interacting protein X1)在宫颈癌细胞系、正常宫颈组织标本、宫颈癌组织标本中的变异情况。方法采用3种宫颈癌细胞系(加以3种淋巴瘤细胞系、1种乳腺癌细胞系作为参照)、28例正常宫颈组织及52例宫颈癌组织标本。提取基因组DNA为模板,PCR扩增PinX1基因全部7个外显子及其部分侧翼序列,PCR纯化产物直接测序从而检测PinX1基因的变异情况,SPSS统计学软件进行数据分析。结果于宫颈癌细胞系中发现3个突变位点,于52例宫颈癌组织中发现5个变异位点,其中位于第7外显子的Ser254Cys(23.1%)及位于第2内含子的IVS2+18 G→A(51.9%)变异率与正常宫颈组织相比有显著差异(P<0.05),位于第2内含子的1个变异位点IVS2+64 C→A(9.3%)为首次报道。结论在宫颈癌中,可能存在PinX1基因的变异新位点,对PinX1基因变异的研究,可能为宫颈癌的诊断、防治和个体化诊疗提供潜在的新靶标。展开更多
Photonic microwave harmonic down-converters (PMHDCs) based on self-oscillation optical frequency combs (OFCs) are interesting because of their broad bandwidth compared with plain optoelectronic oscillators. In this pa...Photonic microwave harmonic down-converters (PMHDCs) based on self-oscillation optical frequency combs (OFCs) are interesting because of their broad bandwidth compared with plain optoelectronic oscillators. In this paper, a high-efficiency and flexible PMHDC is proposed and demonstrated. The properties of the OFC, such as the carrier-to-noise ratio (CNR),bandwidth and free spectral range (FSR), and the influence of optical injection, are investigated. The broadband OFC provides a frequency tunable and high-quality local oscillation (LO), which guarantees flexible down-conversion for the radio frequency (RF) signal. The sideband selective amplification (SSA) effect not only improves the conversion efficiency but also promotes single-sideband modulation. The conversion range can reach 100 GHz. The 12–40 GHz RF signal can be downconverted to intermediate frequency (IF) signals with a high conversion efficiency of 14.9 dB. The fixed 40-GHz RF signal is flexibly down-converted to an IF signal with the frequency from 55.4 to 2129.4 MHz. The phase noise of an IF signal at a frequency offset of 10 kHz is the same as that of the input RF signal. The PMHDC shows great performance and will find applications in radio-over-fiber (RoF) networks, electronic warfare receivers, avionics, and wireless communication systems.展开更多
文摘目的探讨端粒酶抑制因子PinX1(Pin2/TRF1-interacting protein X1)在宫颈癌细胞系、正常宫颈组织标本、宫颈癌组织标本中的变异情况。方法采用3种宫颈癌细胞系(加以3种淋巴瘤细胞系、1种乳腺癌细胞系作为参照)、28例正常宫颈组织及52例宫颈癌组织标本。提取基因组DNA为模板,PCR扩增PinX1基因全部7个外显子及其部分侧翼序列,PCR纯化产物直接测序从而检测PinX1基因的变异情况,SPSS统计学软件进行数据分析。结果于宫颈癌细胞系中发现3个突变位点,于52例宫颈癌组织中发现5个变异位点,其中位于第7外显子的Ser254Cys(23.1%)及位于第2内含子的IVS2+18 G→A(51.9%)变异率与正常宫颈组织相比有显著差异(P<0.05),位于第2内含子的1个变异位点IVS2+64 C→A(9.3%)为首次报道。结论在宫颈癌中,可能存在PinX1基因的变异新位点,对PinX1基因变异的研究,可能为宫颈癌的诊断、防治和个体化诊疗提供潜在的新靶标。
基金supported by the National Natural Science Foundation of China (No. 81070229)Natural Science Foundation Project of Chongqing Science and Technology CommissionChina (No. CSTC 2009BB5139)
基金supported in part by the National Natural Science Foundation of China (Nos.62071487,62201615,62301569,and 62371470)。
文摘Photonic microwave harmonic down-converters (PMHDCs) based on self-oscillation optical frequency combs (OFCs) are interesting because of their broad bandwidth compared with plain optoelectronic oscillators. In this paper, a high-efficiency and flexible PMHDC is proposed and demonstrated. The properties of the OFC, such as the carrier-to-noise ratio (CNR),bandwidth and free spectral range (FSR), and the influence of optical injection, are investigated. The broadband OFC provides a frequency tunable and high-quality local oscillation (LO), which guarantees flexible down-conversion for the radio frequency (RF) signal. The sideband selective amplification (SSA) effect not only improves the conversion efficiency but also promotes single-sideband modulation. The conversion range can reach 100 GHz. The 12–40 GHz RF signal can be downconverted to intermediate frequency (IF) signals with a high conversion efficiency of 14.9 dB. The fixed 40-GHz RF signal is flexibly down-converted to an IF signal with the frequency from 55.4 to 2129.4 MHz. The phase noise of an IF signal at a frequency offset of 10 kHz is the same as that of the input RF signal. The PMHDC shows great performance and will find applications in radio-over-fiber (RoF) networks, electronic warfare receivers, avionics, and wireless communication systems.
