用根癌农杆菌(Agrobacterium tum efaciens)和基因枪两种方法,以桃(Prunus persicaL.Batsch)幼胚子叶为受体,转化桃ACC氧化酶(ACO)反义基因片段,经筛选培养获得了卡那霉素抗性芽。微芽嫁接培养抗性芽部分可成株。PCR、Southern杂交和GU...用根癌农杆菌(Agrobacterium tum efaciens)和基因枪两种方法,以桃(Prunus persicaL.Batsch)幼胚子叶为受体,转化桃ACC氧化酶(ACO)反义基因片段,经筛选培养获得了卡那霉素抗性芽。微芽嫁接培养抗性芽部分可成株。PCR、Southern杂交和GUS基因表达等分子检测,初步表明外源反义ACO基因已经整合到桃基因组中。展开更多
A method for adventitious shoot regeneration from mature cotyledons and leaves of seed-derived Loquat (Eriobotrya japonica cv. ‘Dahongpao’) in vitro has been developed. The results showed that: 1) GA pre-treatments ...A method for adventitious shoot regeneration from mature cotyledons and leaves of seed-derived Loquat (Eriobotrya japonica cv. ‘Dahongpao’) in vitro has been developed. The results showed that: 1) GA pre-treatments could shorten the time of seed germination. 2) Shoots regeneration occurred when mature cotyledons were cultured on MS supplemented with TDZ and GA. The highest adventitious shoot frequency from mature cotyledons was 83.3%. The regeneration was changed when mature cotyledons were wounded by a razor blade and the best results were obtained from the single mature cotyledons with plantule. Shoots could regenerate from mature cotyledons with plantule, but could not get any regeneration of adventitious shoot from the mature cotyledons without plantule. 3) Different part of leaf had different potential of regeneration. Adventitious shoots regenerated from the part which near the leaf-petiole through callus, but the other parts of leaf only got callus. The highest adventitious shoot frequency from the part of leaf-petiole was 65.0%.展开更多
文摘用根癌农杆菌(Agrobacterium tum efaciens)和基因枪两种方法,以桃(Prunus persicaL.Batsch)幼胚子叶为受体,转化桃ACC氧化酶(ACO)反义基因片段,经筛选培养获得了卡那霉素抗性芽。微芽嫁接培养抗性芽部分可成株。PCR、Southern杂交和GUS基因表达等分子检测,初步表明外源反义ACO基因已经整合到桃基因组中。
文摘A method for adventitious shoot regeneration from mature cotyledons and leaves of seed-derived Loquat (Eriobotrya japonica cv. ‘Dahongpao’) in vitro has been developed. The results showed that: 1) GA pre-treatments could shorten the time of seed germination. 2) Shoots regeneration occurred when mature cotyledons were cultured on MS supplemented with TDZ and GA. The highest adventitious shoot frequency from mature cotyledons was 83.3%. The regeneration was changed when mature cotyledons were wounded by a razor blade and the best results were obtained from the single mature cotyledons with plantule. Shoots could regenerate from mature cotyledons with plantule, but could not get any regeneration of adventitious shoot from the mature cotyledons without plantule. 3) Different part of leaf had different potential of regeneration. Adventitious shoots regenerated from the part which near the leaf-petiole through callus, but the other parts of leaf only got callus. The highest adventitious shoot frequency from the part of leaf-petiole was 65.0%.
