A new species Myxobolus guanqiaoensis was found in the liver and abdominalcavity of Allogynogenetic silver prassian carp at Guanqiao, in Oct l996. The typespecimens were preserved in the Laboratory of Hsh ixseases, In...A new species Myxobolus guanqiaoensis was found in the liver and abdominalcavity of Allogynogenetic silver prassian carp at Guanqiao, in Oct l996. The typespecimens were preserved in the Laboratory of Hsh ixseases, InshtUte of Hydrobiology,The Chinese Academy of Sciences, Wuhan, China.apcobolus guanqiaoensis sp. nov.Host and site: Allogynogenehc si1ver crucian carp (Carassius auratus gibelio l XCyPrinus carP io J), Liver and abdowhnal cavity.Locality: Guanqiao, Wuhan, China.Infechon ratet above 6o%.Cystt Oval or elliptic in fOrm, white in color; 2.l-4.Ocm in diametef, embracedby a tf8nsparent membrane.Sporest Spores elongatedsioval in thecal view. Pointed at anterior end and blunhyround in posterior end; l-3 V-shaPed folds at posterior end; elongated elliphc insutural view, sutUral line S-shaPed and dishnct shell valves smooth; intercaPsularaPPendix dishnct. Two polar capsules eggplant unequal in size, polar caPsule locatedat l / 2 position of spore length; 7-8pm flat coiled filamenLChmensions of 2o fresh spores f tength l9.6(l8.2-22.o)pm. width Io.8(9.O-l3.2)pm. thickness 8.3(8.O-9.o)Um, large po1ar capsules 9.5(8.8-lO.l) by 3.7(3.O-4.4)Um,small polar caPsules 8.4(7.9-9.8) by 3.6(3.2-4.O)Um. Polar caPsule nuclei sitUated atthe inner of tWo polar capsules. An indistinct iodinophilous vacuole and two roundgerminal nuclei.The shape of trhis new sPecies is similar to apcobolus gigi, M)Af)bolus kDi andapcobolus aureatus, but differs from them in dimensions of sPore, the size of POlarcapsule, suture line and host.展开更多
The aim was to develop a cell culture system capable of producing high titer hepatitis C virus(HCV) stocks with recombinant vaccinia viruses as helpers.Two plasmids were used for the generation of recombinant HCV:one ...The aim was to develop a cell culture system capable of producing high titer hepatitis C virus(HCV) stocks with recombinant vaccinia viruses as helpers.Two plasmids were used for the generation of recombinant HCV:one containing the full\|length HCV cDNA cloned between the T7 promoter and T7 terminator of the pOCUS\|T7 vector;and the other containing the HCV polyprotein open reading frame(ORF) directly linked to a vaccinia late promoter in the PSC59.These two plasmids were co\|transfected into BHK 21 cells,which were then infected with vTF7\|3 recombinant vaccinia helper viruses.After 5 days incubation,approximately 3 60±0 18×10 7 copies of HCV RNA were present per ml of cell culture supernatant,as detected by fluorescence quantitative RT\|PCR(FQ\|PCR).The yield of recombinant HCV using this cell system increased 10～100 fold compare to in vitro\| transcribed HCV genomic RNA or selectable subgenomic HCV RNA molecules method.These results suggest that this cell culture system is capable of producing high titer recombinant HCV. Furthermore,it may be useful as a system for future drug screening and vaccine selection.;展开更多
文摘A new species Myxobolus guanqiaoensis was found in the liver and abdominalcavity of Allogynogenetic silver prassian carp at Guanqiao, in Oct l996. The typespecimens were preserved in the Laboratory of Hsh ixseases, InshtUte of Hydrobiology,The Chinese Academy of Sciences, Wuhan, China.apcobolus guanqiaoensis sp. nov.Host and site: Allogynogenehc si1ver crucian carp (Carassius auratus gibelio l XCyPrinus carP io J), Liver and abdowhnal cavity.Locality: Guanqiao, Wuhan, China.Infechon ratet above 6o%.Cystt Oval or elliptic in fOrm, white in color; 2.l-4.Ocm in diametef, embracedby a tf8nsparent membrane.Sporest Spores elongatedsioval in thecal view. Pointed at anterior end and blunhyround in posterior end; l-3 V-shaPed folds at posterior end; elongated elliphc insutural view, sutUral line S-shaPed and dishnct shell valves smooth; intercaPsularaPPendix dishnct. Two polar capsules eggplant unequal in size, polar caPsule locatedat l / 2 position of spore length; 7-8pm flat coiled filamenLChmensions of 2o fresh spores f tength l9.6(l8.2-22.o)pm. width Io.8(9.O-l3.2)pm. thickness 8.3(8.O-9.o)Um, large po1ar capsules 9.5(8.8-lO.l) by 3.7(3.O-4.4)Um,small polar caPsules 8.4(7.9-9.8) by 3.6(3.2-4.O)Um. Polar caPsule nuclei sitUated atthe inner of tWo polar capsules. An indistinct iodinophilous vacuole and two roundgerminal nuclei.The shape of trhis new sPecies is similar to apcobolus gigi, M)Af)bolus kDi andapcobolus aureatus, but differs from them in dimensions of sPore, the size of POlarcapsule, suture line and host.
文摘The aim was to develop a cell culture system capable of producing high titer hepatitis C virus(HCV) stocks with recombinant vaccinia viruses as helpers.Two plasmids were used for the generation of recombinant HCV:one containing the full\|length HCV cDNA cloned between the T7 promoter and T7 terminator of the pOCUS\|T7 vector;and the other containing the HCV polyprotein open reading frame(ORF) directly linked to a vaccinia late promoter in the PSC59.These two plasmids were co\|transfected into BHK 21 cells,which were then infected with vTF7\|3 recombinant vaccinia helper viruses.After 5 days incubation,approximately 3 60±0 18×10 7 copies of HCV RNA were present per ml of cell culture supernatant,as detected by fluorescence quantitative RT\|PCR(FQ\|PCR).The yield of recombinant HCV using this cell system increased 10～100 fold compare to in vitro\| transcribed HCV genomic RNA or selectable subgenomic HCV RNA molecules method.These results suggest that this cell culture system is capable of producing high titer recombinant HCV. Furthermore,it may be useful as a system for future drug screening and vaccine selection.;
