本文报道10～40μg/ml NiSO_46H_2O(Ni)在试管中能使 FA 细胞株的 EB 病毒(EBV)核抗原(EBNA-2)表达增强10.99～39.29%。1～20μg/ml Ni 有明显促进 EBV 转化人脐血 B 淋巴细胞的作用,当多次加Ni 时促进效应更强,前者转化促进率为13.31～...本文报道10～40μg/ml NiSO_46H_2O(Ni)在试管中能使 FA 细胞株的 EB 病毒(EBV)核抗原(EBNA-2)表达增强10.99～39.29%。1～20μg/ml Ni 有明显促进 EBV 转化人脐血 B 淋巴细胞的作用,当多次加Ni 时促进效应更强,前者转化促进率为13.31～46.61,后者达22.70～57.04%。最佳浓度为10μg/ml。EBV 与鼻咽癌(NPC)密切相关,广东 NPC 高发区粮食和饮水中 Ni 的含量偏高,NPC 患者血和头发中 Ni 的水平明显高于健康人。研究结果提示,EBV 与 Ni 相互作用可能是 NPC 发生,发展过程中的一个协同因素。展开更多
This work was supported by the National Natural Science Foundation of China (No. 39370766). Object: To study the existent form of EBV genome in nasopharyngeal carcinoma (NPC) biopsies, in a trans plan...This work was supported by the National Natural Science Foundation of China (No. 39370766). Object: To study the existent form of EBV genome in nasopharyngeal carcinoma (NPC) biopsies, in a trans planted NPC tumor SUNT 1 and its corresponding epithelial cell line SUNE 1. Methods: By using polymerase chain reaction (PCR) amplification of Epstein Barr virus (EBV) BamHI W fragment, EBV DNA was detected in 20/20 biopsy specimens of poorly differentiated, as well as in a nude mouse xenografted NPC tumor (SUNT 1, from passage 1 to 34) and in the corresponding epithelial cell line (SUNE 1, from passage 1 to 62). The intracellular form of EBV genome was studied by analyzing the terminal structure using a LMP2A probe and an “ in situ lysing gel” technique. Results: A single EBV fused terminal DNA fragment was detected in 19 biopsy specimens, two hybridized bands were seen in one specimen. These results indicate that an episomal form of EBV genome is predominantly present in most NPC biopsy specimens, but insertion of the genome into the host chromosome could not be excluded. Conclusion: The finding suggests that EBV infection precedes clonal amplification of transformed cells, or in a rare case, that a single EBV infected clone is predominant in the development of NPC. Linear form of EBV DNA was detected in the 20th passage of SUNE 1; this may imply the in vitro activation of the productive cycle of EBV.展开更多
文摘本文报道10～40μg/ml NiSO_46H_2O(Ni)在试管中能使 FA 细胞株的 EB 病毒(EBV)核抗原(EBNA-2)表达增强10.99～39.29%。1～20μg/ml Ni 有明显促进 EBV 转化人脐血 B 淋巴细胞的作用,当多次加Ni 时促进效应更强,前者转化促进率为13.31～46.61,后者达22.70～57.04%。最佳浓度为10μg/ml。EBV 与鼻咽癌(NPC)密切相关,广东 NPC 高发区粮食和饮水中 Ni 的含量偏高,NPC 患者血和头发中 Ni 的水平明显高于健康人。研究结果提示,EBV 与 Ni 相互作用可能是 NPC 发生,发展过程中的一个协同因素。
文摘This work was supported by the National Natural Science Foundation of China (No. 39370766). Object: To study the existent form of EBV genome in nasopharyngeal carcinoma (NPC) biopsies, in a trans planted NPC tumor SUNT 1 and its corresponding epithelial cell line SUNE 1. Methods: By using polymerase chain reaction (PCR) amplification of Epstein Barr virus (EBV) BamHI W fragment, EBV DNA was detected in 20/20 biopsy specimens of poorly differentiated, as well as in a nude mouse xenografted NPC tumor (SUNT 1, from passage 1 to 34) and in the corresponding epithelial cell line (SUNE 1, from passage 1 to 62). The intracellular form of EBV genome was studied by analyzing the terminal structure using a LMP2A probe and an “ in situ lysing gel” technique. Results: A single EBV fused terminal DNA fragment was detected in 19 biopsy specimens, two hybridized bands were seen in one specimen. These results indicate that an episomal form of EBV genome is predominantly present in most NPC biopsy specimens, but insertion of the genome into the host chromosome could not be excluded. Conclusion: The finding suggests that EBV infection precedes clonal amplification of transformed cells, or in a rare case, that a single EBV infected clone is predominant in the development of NPC. Linear form of EBV DNA was detected in the 20th passage of SUNE 1; this may imply the in vitro activation of the productive cycle of EBV.
