N-terminal and C-terminal analysis of genetic engineering recombinant proteins are two criteria of determining their primary structure. The N-terminal sequencing relies heavily upon the Edman degradation method, which...N-terminal and C-terminal analysis of genetic engineering recombinant proteins are two criteria of determining their primary structure. The N-terminal sequencing relies heavily upon the Edman degradation method, which has been automated with commercially available sequencers. The maximum number of residues which may be placed in sequence in a single run is about 50. In the present decade, improvements in this technique made it展开更多
文摘N-terminal and C-terminal analysis of genetic engineering recombinant proteins are two criteria of determining their primary structure. The N-terminal sequencing relies heavily upon the Edman degradation method, which has been automated with commercially available sequencers. The maximum number of residues which may be placed in sequence in a single run is about 50. In the present decade, improvements in this technique made it
