Objective： To determine whether AQP4 expression is associated with lactate （Lac） and Nacetyl aspartate （NAA） and with apparent diffusion coefficient （ADC） abnormality after rat cerebral ischemia. Methods： The ...Objective： To determine whether AQP4 expression is associated with lactate （Lac） and Nacetyl aspartate （NAA） and with apparent diffusion coefficient （ADC） abnormality after rat cerebral ischemia. Methods： The time courses of ADC and lactate and NAA assessed by proton magnetic resonance spectroscopy （^1HMRS） were investigated at the time point of 6 h, and 1, 3, 7 d after rat cerebral ischernia induced by middle cerebral artery occlusion. Expression of AQP4 mRNA and protein were measured using RT-PCR and Western blot analysis respectively. Results： Significant reductions of NAA concentration and increases of lactate concentration were found after rat cerebral ischemia. The expressions of AQP4 mRNA t and protein were increased at 6 h, and reached the peak at 1-3 d, then began to decrease at 7 d after rat cerebral ischemia. The expression of AQP4 was significantly correlated with NAA （rRT = -0. 856, rw = -0. 927, P〈0.01）, and with lactate （rw=0. 473, rRT=0. 413, P〈0.05）, and with ADC values during the period of 1-7d after rat cerebralischemia （rw=0.984, rRT=0.925, P〈0.05）. In addition, correlations between Lac and the ADC values（r=-0. 677, P〈0.05）and between NAA and ADC values during the period of 1-7 d after rat cerebralischemia （r=0.909, P（0.05） were also observed. Conclusion： The data suggest that AQP4 is involved in the transport of water when brain edema is formed and cell membrane integrity is lost.展开更多
文摘Objective： To determine whether AQP4 expression is associated with lactate （Lac） and Nacetyl aspartate （NAA） and with apparent diffusion coefficient （ADC） abnormality after rat cerebral ischemia. Methods： The time courses of ADC and lactate and NAA assessed by proton magnetic resonance spectroscopy （^1HMRS） were investigated at the time point of 6 h, and 1, 3, 7 d after rat cerebral ischernia induced by middle cerebral artery occlusion. Expression of AQP4 mRNA and protein were measured using RT-PCR and Western blot analysis respectively. Results： Significant reductions of NAA concentration and increases of lactate concentration were found after rat cerebral ischemia. The expressions of AQP4 mRNA t and protein were increased at 6 h, and reached the peak at 1-3 d, then began to decrease at 7 d after rat cerebral ischemia. The expression of AQP4 was significantly correlated with NAA （rRT = -0. 856, rw = -0. 927, P〈0.01）, and with lactate （rw=0. 473, rRT=0. 413, P〈0.05）, and with ADC values during the period of 1-7d after rat cerebralischemia （rw=0.984, rRT=0.925, P〈0.05）. In addition, correlations between Lac and the ADC values（r=-0. 677, P〈0.05）and between NAA and ADC values during the period of 1-7 d after rat cerebralischemia （r=0.909, P（0.05） were also observed. Conclusion： The data suggest that AQP4 is involved in the transport of water when brain edema is formed and cell membrane integrity is lost.
