The heteronuclear gadolinium copper complex was synthesized. Its crystal structure was determined by single crystal diffraction. The crystal crystallizes in triclinic, space group P 1, a=0 8483(1) nm, b =0 ...The heteronuclear gadolinium copper complex was synthesized. Its crystal structure was determined by single crystal diffraction. The crystal crystallizes in triclinic, space group P 1, a=0 8483(1) nm, b =0 9642(2) nm, c =1 2838(2) nm, α =111 17(1)°, β = 93 52(1)°, γ =100 89(1)°, Z =2, D c=2 335 g·cm -3 . The complex appears as a zigzag 1D chain. Each gadolinium ion is coordinated by three carboxylic oxygen atoms and six oxygen atoms from H 2O. Each copper ion is chelated by NTA with its nitrogen atom and three oxygen atoms of three carboxyl groups, and coordinated by a chlorine ion. The ClO 4 - is located between the chains as a counter ion.展开更多
Objective. To study the characteristics of changes of LDH enzyme patterns of mice under slight hypoxia. Methods. Mice treated with artificial hypoxia, various tissues were made for the test of LDH enzymatic activity b...Objective. To study the characteristics of changes of LDH enzyme patterns of mice under slight hypoxia. Methods. Mice treated with artificial hypoxia, various tissues were made for the test of LDH enzymatic activity by the specific staining technique. LDH(1-5) relative percentage enzymatic activity (RPEA) were measured with CS-910 dual-wavelength thin layer chromatography scanner. Results. The RPEA of LDH isozymes of various tissues after slight hypoxia shifted to the isozymes LDH1 and LDH2, whose principal subunits are H subunits, and be RPEA of LDH1(H4), LDH2(H3M) increased, while RPEA of LDH5(M4) in various tissues decreased prominently except the cardiac muscle, and that of LDH4(HM3) decreased as well. After polyacrylamide gel electrophoresis (PAGE) of the hypoxia treated cardiac muscle specimen was made, activity subbands originated regularly in the isozyme patterns of LDH, with the regularity of LDH1 (0 subband), LDH2 (0-1 subbands), LDH3(0-2 subbands), LDH4(1-3 subbands), LDH5(2-4 subbands). After adding appropriate amount of NAD^+ to the hypoxia treated cardiac muscle specimen, PAGE showed the subbands of four isozymes (LDH2-LDH5) reduced or even totally disappeared in the isozyme patterns. Conclusions. The negative feedback regulation of coenzymization and decoenzymization of LDH isozymes is one of the mouse stress responses to slight hypoxia.展开更多
Phase transition of a lipid-like hemicyanine compound characterized by second harmonic generation is studied carefully. The phase transition is assigned as the first order transition between solid state and liquid sta...Phase transition of a lipid-like hemicyanine compound characterized by second harmonic generation is studied carefully. The phase transition is assigned as the first order transition between solid state and liquid state. The transition temperature increases with an increase in the surface molecular concentration. A monolayer structure parameter a which is very sensitive to the phase transition is introduced.展开更多
文摘The heteronuclear gadolinium copper complex was synthesized. Its crystal structure was determined by single crystal diffraction. The crystal crystallizes in triclinic, space group P 1, a=0 8483(1) nm, b =0 9642(2) nm, c =1 2838(2) nm, α =111 17(1)°, β = 93 52(1)°, γ =100 89(1)°, Z =2, D c=2 335 g·cm -3 . The complex appears as a zigzag 1D chain. Each gadolinium ion is coordinated by three carboxylic oxygen atoms and six oxygen atoms from H 2O. Each copper ion is chelated by NTA with its nitrogen atom and three oxygen atoms of three carboxyl groups, and coordinated by a chlorine ion. The ClO 4 - is located between the chains as a counter ion.
文摘Objective. To study the characteristics of changes of LDH enzyme patterns of mice under slight hypoxia. Methods. Mice treated with artificial hypoxia, various tissues were made for the test of LDH enzymatic activity by the specific staining technique. LDH(1-5) relative percentage enzymatic activity (RPEA) were measured with CS-910 dual-wavelength thin layer chromatography scanner. Results. The RPEA of LDH isozymes of various tissues after slight hypoxia shifted to the isozymes LDH1 and LDH2, whose principal subunits are H subunits, and be RPEA of LDH1(H4), LDH2(H3M) increased, while RPEA of LDH5(M4) in various tissues decreased prominently except the cardiac muscle, and that of LDH4(HM3) decreased as well. After polyacrylamide gel electrophoresis (PAGE) of the hypoxia treated cardiac muscle specimen was made, activity subbands originated regularly in the isozyme patterns of LDH, with the regularity of LDH1 (0 subband), LDH2 (0-1 subbands), LDH3(0-2 subbands), LDH4(1-3 subbands), LDH5(2-4 subbands). After adding appropriate amount of NAD^+ to the hypoxia treated cardiac muscle specimen, PAGE showed the subbands of four isozymes (LDH2-LDH5) reduced or even totally disappeared in the isozyme patterns. Conclusions. The negative feedback regulation of coenzymization and decoenzymization of LDH isozymes is one of the mouse stress responses to slight hypoxia.
基金Project supported by the National Natural Science Foundation of China (Grant Nos. 29425004, 19434012 and 29671001)
文摘Phase transition of a lipid-like hemicyanine compound characterized by second harmonic generation is studied carefully. The phase transition is assigned as the first order transition between solid state and liquid state. The transition temperature increases with an increase in the surface molecular concentration. A monolayer structure parameter a which is very sensitive to the phase transition is introduced.
