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2008年全国疟疾形势 被引量:74
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作者 周水森 王漪 +1 位作者 房文 汤林华 《中国寄生虫学与寄生虫病杂志》 CAS CSCD 北大核心 2009年第6期455-457,共3页
本文根据2008年全国有疟疾发病的22个省(市、区)专业单位上报的年度疟疾防治工作总结和有关疫情报表(年报系统)汇总整理,除特别注明“网络直报”外,所有疫情数据均来自年报系统。
关键词 中国 疟疾 报告病例
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2003年全国疟疾形势 被引量:32
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作者 周水森 汤林华 盛慧锋 《中国寄生虫学与寄生虫病杂志》 CAS CSCD 北大核心 2005年第6期385-387,共3页
Total 40 681 malaria cases and 129 147 suspected cases with 52 deaths were reported by the case reporting system in 910 counties of 21 Provinces/Municipality/Autonomous Region(P/M/A) in 2003,and the annual incidence w... Total 40 681 malaria cases and 129 147 suspected cases with 52 deaths were reported by the case reporting system in 910 counties of 21 Provinces/Municipality/Autonomous Region(P/M/A) in 2003,and the annual incidence was 0.39/10 000,an increase of 15.3% than that of the last year,which is the third year that malaria incidence consecutively increased since 2001.Based on a baseline survey in the project funded by the Global Fund to Fight AIDS,Tuberculosis and Malaria(GFATM),the estimated number of malaria cases was about 740 000 in 2003,18 times more than reported. Among the 910 counties with reported malaria cases,29 counties with an incidence more than 10/10 000 distributed in Yunnan(17 counties),Hainan(9),Anhui(1),Hubei(1) and Henan(1).There were 69 counties in which the malaria incidence was between 1/10 000 and 10/10 000. The number of Plasmodium falciparum malaria cases was 4 727,accounting for 11.6% of the total cases,of which 13.1%(621) were imported cases reported in 107 counties/cities of 16 P/M/A.Indigenous falciparum malaria was found in 78 counties/cities of Yunnan and Hainan Provinces,of which 64 counties/cities were in Yunnan,increased by 22,14 counties/cities were in Hainan,decreased by 2 compared to that of 2002. Yunnan and Hainan are still the relatively high transmission areas.Yunnan has ranked No.1 in the country in terms of the number of cases while Hainan ranked No.1 by malaria incidence in recent years.21 788 malaria cases were reported from the two provinces in 2003,accounting for 53.6% of the total reported cases in the country.There were 15 431 cases with 43 deaths reported from Yunnan,the incidence was 4.24/10 000,with an increase of 26.3% than that in the last year.Among the reported cases,3 529 were falciparum malaria,increased by 22.9% in comparison to 2002.The number of reported cases in Hainan was 6 357,with an incidence of 7.94/10 000,19.6% increase than the last year. In central China,the re,emergence of malaria was considerable in provinces along the Huai River,especially in Anhui Province.It should be stressed that the proportion of malaria cases was increasing in Anopheles sinensis transmitted areas where malaria was almost under control in the 1990s.The number of malaria cases in Anhui has been the second largest in the country since 2001.8 025 malaria cases and 18 533 suspected cases were reported from Anhui in 2003,accounting for 19.7% of the total cases in the country,with an incidence of 1.53/10 000 increased by 59.4% than that in 2002.Hubei Province reported 5 334 malaria cases with an incidence of 1.2/10 000,increased by 33.3%.The number of reported cases in Henan and Jiangsu Provinces was 2 448 and 638 and the incidence decreased by 24.1% and 12.7% respectively.Focal outbreaks occurred in 222 villages of 9 counties in Anhui,Hubei and Jiangsu,where Anopheles sinensis is the principal transmission vector.Malaria cases reported from the above 4 provinces accounted for over 40% of the national figure. Cases reported from other P/M/A in the South and East China occupied about 6% of the total with certain degree of decrease,several hundreds from each of Guizhou,Guangxi,Sichuan,Guangdong,Fujian,Chongqing,Zhejiang,Shanghai and Hunan,more than 50% of which were imported cases.Less than 100 cases were reported from each of Shandong,Jiangxi,Liaoning,Shaanxi,Shanxi and Gansu Provinces in 2003. In summary,malaria is still an important problem of public health in China,especially in the southern and central parts where the incidence has been increasing since 2001.Yunnan and Hainan still faced critical situation of malaria endemics with a spread of Plasmodium falciparum,especially in the 25 border counties in Yunnan.In the central part of the country including Anhui,Hubei,Henan and Jiangsu,where Anopheles sinensis was the principal vector,the malaria prevalence was highly unstable with frequent focal outbreaks in areas along the Huai River,which revealed new challenges to the malaria control program in China.Meanwhile,opportunities also exist with the support of the GFATM and the government,the latter paid much closer attention recently to issues in relation to public health. 展开更多
关键词 疟疾 病例报告 局部爆发 发病数 不同程度 出现 上升 安徽 云南 湖北
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2007年全国疟疾形势 被引量:54
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作者 周水森 王漪 +1 位作者 房文 汤林华 《中国寄生虫学与寄生虫病杂志》 CAS CSCD 北大核心 2008年第6期401-403,共3页
本文根据2007年全国有疟疾发病的23省(市、区)专业单位上报的年度疟疾防治工作总结和有关疫情报表(年报系统)汇总整理,除特别注明外,所有疫情数据均来自年报系统。
关键词 中国 疟疾 报告病例
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不同地区微小按蚊rDNA-ITS2序列差异 被引量:20
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作者 周水森 汤林华 +1 位作者 顾政诚 王漪 《中国寄生虫学与寄生虫病杂志》 CAS CSCD 北大核心 2002年第1期29-31,共3页
目的 比较我国云南、海南省、广西壮族自治区及泰国等不同地区微小按蚊核糖体 DNA第 2内转录间隔区 (ITS2 )序列差异。 方法 取单只雌蚊蚊腿消化提取 DNA,PCR特异扩增 r DNA- ITS2片段 ,并对其扩增产物纯化、测序及分析。 结果 发... 目的 比较我国云南、海南省、广西壮族自治区及泰国等不同地区微小按蚊核糖体 DNA第 2内转录间隔区 (ITS2 )序列差异。 方法 取单只雌蚊蚊腿消化提取 DNA,PCR特异扩增 r DNA- ITS2片段 ,并对其扩增产物纯化、测序及分析。 结果 发现 2种不同的 ITS2序列 (Gen Bank登录号 :AF4 16 783,AF4 16 784 ) ,分别与微小按蚊 A和 C同源 ,ITS2序列长度及 GC含量分别为 4 81bp,5 4 .0 4 %和 4 83bp,5 4 .2 3% ,两者无显著性差异 ;但 2 2个固定位点存在碱基置换和插入 /缺失 ,序列差异为 5 .8%。 结论 研究区内微小按蚊存在 A和 展开更多
关键词 微小按蚊 核糖体DNA 第2内转录间隔区 RDNA-ITS2 序列差异
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2010年全国疟疾疫情分析 被引量:133
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作者 周水森 王漪 李雨 《中国寄生虫学与寄生虫病杂志》 CAS CSCD 北大核心 2011年第6期401-403,共3页
本文根据2010年全国有疟疾报告的23个省(市、区)专业单位上报的年度疟疾防治工作总结和有关疫情报表(年报系统)汇总整理。
关键词 中国 疟疾 报告病例
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2009年全国疟疾疫情分析 被引量:94
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作者 周水森 王漪 夏志贵 《中国寄生虫学与寄生虫病杂志》 CAS CSCD 北大核心 2011年第1期1-3,共3页
本文根据2009年全国有疟疾发病的23省(市、区)专业单位上报的年度疟疾防治工作总结和有关疫情报表(年报系统)汇总整理,除特别注明"网络直报"外,所有疫情数据均来自年报系统。
关键词 中国 疟疾 报告病例
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2004年全国疟疾形势 被引量:27
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作者 周水森 汤林华 +1 位作者 盛慧锋 王漪 《中国寄生虫学与寄生虫病杂志》 CAS CSCD 北大核心 2006年第1期1-3,共3页
In 2004, {38 972} malaria cases and {106 704} suspected cases with 31 deaths were reported by the annual case reporting system in {1 005} counties of 23 Provinces/Municipality/Autonomous Region (P/M/A) and the annual ... In 2004, {38 972} malaria cases and {106 704} suspected cases with 31 deaths were reported by the annual case reporting system in {1 005} counties of 23 Provinces/Municipality/Autonomous Region (P/M/A) and the annual incidence was 0^38/{10 000}, a decrease of 4^2% than that of the last year, which is the year that the disease was slightly rolled back after consecutively increased since 2001. As an important development of the case reporting system,the internet reporting system started in 2004,majority of the malaria cases ({38 254}) were reported through internet from {1 074} counties of all the 30 P/M/A except Qinghai Province. ;Among the {1 005} counties with reported malaria cases, 32 counties were with an incidence of more than 10/{10 000} distributing in Yunnan (19 counties), Hainan (10) and Anhui (3). There were 61 counties in which the malaria incidence was between 1/{10 000} and 10/{10 000}.The number of Plasmodium falciparum malaria cases was 4 891, accounting for 12^6% of the total cases, of which 35^0% (1 714) were imported cases reported in 174 counties/cities of 18 P/M/A. Indigenous falciparum malaria was found in 45 counties/cities of Yunnan and Hainan Provinces, of which 29 counties/cities were in Yunnan, decreased by 35, 16 were in Hainan, increased by 2 compared to that of 2003.;Yunnan and Hainan Provinces are still the relatively high transmission areas. Yunnan has ranked No^1 in the country in terms of the number of cases while Hainan ranked No^1 by malaria incidence in recent years. {22 247} malaria cases were reported from the two provinces in 2004, accounting for 57^1% of the total reported cases in the country. There were {12 865} cases with 25 deaths reported from Yunnan, and the incidence was 3^09/{10 000}, a decrease of 16^6% than that in the last year. Among the reported cases, {3 251} were falciparum malaria, decreased by 7^9% in comparison to 2003. The number of reported cases in Hainan was 9 832, with an incidence of 11^64/{10 000}, 46^6% increase than the last year.;In central China, the re-emergence of malaria was considerable in provinces along the Huai River, especially in Anhui Province. The case number in Anhui has been the second or third largest in the country since 2001. {8 909} malaria cases and {14 542} suspected cases were reported from the Province in 2004, accounting for 22^9% of the total cases in the country, with an incidence of 1^38/{10 000} increased by 9^8% than that in 2003. Hubei reported {2 714} malaria cases with an incidence of 0^47/{10 000}, decreased by 60^8%. The number of reported cases in Henan was 2 211, decreased by 43^1%. 676 cases were reported from Jiangsu, almost the same as that in 2003. Focal outbreaks occurred in 109 villages of 13 counties in Anhui, Hubei and Jiangsu, where Anopheles sinensis is the principal transmission vector. Malaria cases reported from the above 4 provinces accounted for 37^2% of the national figure. ;Cases reported from Guizhou, Sichuan, Guangxi, Guangdong, Zhejiang, Shanghai and Hunan in southeast China occupied about 5% of the total cases, over 50% of which were imported cases. Less than 100 cases were reported from each of Fujian, Chongqing, Shandong, Jiangxi, Liaoning, Shaanxi, Shanxi, Gansu, Xinjiang and Heilongjiang Provinces in the year. ;In summary, although malaria cases slightly decreased after several years’ consecutive increase, malaria is still an important problem of public health in China, especially in the southern and central parts. Yunnan and Hainan still face a critical situation of malaria endemics with the spread of P.falciparum, especially in the 25 border counties in Yunnan. In central parts of the country, especially in Anhui, malaria prevalence was highly unstable with frequent focal outbreaks in areas along the Huai River, which reveals new challenges to the national malaria control program. 展开更多
关键词 中国 疟疾 疫情报告
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2006年全国疟疾形势 被引量:73
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作者 周水森 王漪 汤林华 《中国寄生虫学与寄生虫病杂志》 CAS CSCD 北大核心 2007年第6期439-441,共3页
本文根据2006年全国有疟疾发病的23个省(市、区)专业单位上报的年度疟疾防治工作总结和有关疫情报表(年报系统)汇总整理,除特别注明“网络直报系统”外,所有疫情数据均来自年报系统。
关键词 中国 疟疾 报告病例
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2005年全国疟疾形势 被引量:66
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作者 周水森 王漪 汤林华 《中国寄生虫学与寄生虫病杂志》 CAS CSCD 北大核心 2006年第6期401-403,共3页
Totally,42 319 malaria cases and 57 787 suspected cases with 48 deaths were reported by the Annual Case Repor-ting System in 1186 counties of 21 Provinces/Municipality/Autonomous Region(P/M/A) in 2005,and the annual i... Totally,42 319 malaria cases and 57 787 suspected cases with 48 deaths were reported by the Annual Case Repor-ting System in 1186 counties of 21 Provinces/Municipality/Autonomous Region(P/M/A) in 2005,and the annual incidence was 0.59/10 000,an increase of 55.3% than that of the last year.Through the Case Reporting Information System,an internet reporting process started in 2004,40 371 malaria cases were reported from 1 075 counties of 31 P/M/A.The number of malaria cases and the rank of P/M/A were basically in concordance by the two systems.Among the 1186 counties with reported malaria cases,27 counties had an incidence of more than 10/10 000 distributed in Yunnan(15 counties),Hainan(7 counties) and Anhui(5 counties).There were 80 counties in which the malaria incidence was between 1/10 000 and 10/10 000.The number of Plasmodium falciparum malaria cases was 4 146,accounting for 9.8% of the total cases,of which 63.5%(2 632) were imported cases in 178 counties/cities of 18 P/M/A.Indigenous falciparum malaria was found in 47 counties/cities of Yunnan and Hainan Provinces,of which 31 counties/cities were in Yunnan,increased by 29,16 counties/cities were in Hainan,same with that of 2004.Yunnan and Hainan Provinces are still relatively high transmission areas.Yunnan ranked No.2 in the country in terms of the number of cases while Hainan ranked No.1 by malaria incidence in 2005.19 588 malaria cases were reported from the two provinces in 2005,accounting for 46.3% of the total reported cases in the country.There were 15 072 cases with 38 deaths reported from Yunnan,the incidence was 4.95/10 000,an increase of 60.2% than that in the last year.Among the reported cases,3 497 were falciparum malaria with 69.0% imported from the bordering nations.The number of reported cases in Hainan was 4 516,with an incidence of 5.46/10 000,53.1% decrease than the last year.In central China,the resurgence of malaria was considerable in provinces along the Huai River,especially in Anhui Province.The number of malaria cases in Anhui came to the highest in the country in 2005,with 15 681 malaria cases and 7 662 suspected cases reported,accounting for 37.5% of the total cases in the country,and an incidence of 2.45/10 000,increased by 77.5% than that in 2004.The number of reported cases in Henan Province was 2 349,increased by 55.2% in incidence.Hubei Province reported 1 564 malaria cases with an incidence of 0.27/10 000,decreased by 42.6%.651 cases were reported from Jiangsu Province,almost the same as that in 2004.Focal outbreaks occurred in 277 villages of 20 counties in Anhui,Hubei and Jiangsu,where Anopheles sinensis is the principal transmission vector.Malaria cases reported from the above 4 provinces accounted for 47.8% of the national figure,increased approximately by 10% than the last year.Cases reported from P/M/A in the South and East China occupied about 4.9% of the total,similar to that in 2004.Several hundreds were reported from each of Guizhou,Sichuan,Guangxi,Guangdong,Zhejiang,Shanghai and Hunan.Less than 100 cases were reported from each of Fujian,Chongqing,Shandong,Shanghai,Jiangxi,Liaoning,Shaanxi,Shanxi and Gansu Provinces in 2005.High attention should be paid to Guizhou and Tibet.Focal outbreaks occurred in Guizhou for consecutive 4 years,and malaria cases in 2005 double increased than the last year.77.8% of the reported cases were indigenous patients,which implied that local transmission resulting from imported malaria cases has become a major problem in Guizhou.93 malaria cases were reported from Linzhi District of the Tibetan Autonomous Region in 2005,76 cases more than that in 2004.In summary,malaria is still an important problem of public health in China,especially in the southern and central parts.Yunnan and Hainan still faced a severe situation of malaria endemics with the spread of Plasmodium falciparum,especially imported malaria in the 25 border counties in Yunnan.In the central part of the country,especially Anhui Province,the malaria prevalence was highly unstable with frequent focal outbreaks in areas along the Huai River,which revealed new challenges to the malaria control program in China.In addition,more attention needs to be paid to the malaria control in Guizhou and Tibet. 展开更多
关键词 中国 疟疾 病例报告
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运用Kriging法对我国黄淮流域疟疾空间分布特征的研究 被引量:17
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作者 周水森 黄芳 +4 位作者 汤林华 郑香 沈毓祖 苏云普 黄光全 《中国病原生物学杂志》 CSCD 2007年第3期204-206,F0003,共4页
目的研究我国黄淮流域疟疾空间分布特征。方法收集安徽、河南及湖北省沿黄淮流域地区2005年有疟疾报告的156个市、县的当年发病率资料,利用Arcgis 9.0软件建立疟疾发病的地理信息系统,并在该软件的统计学扩展模块支持下,利用Kriging法... 目的研究我国黄淮流域疟疾空间分布特征。方法收集安徽、河南及湖北省沿黄淮流域地区2005年有疟疾报告的156个市、县的当年发病率资料,利用Arcgis 9.0软件建立疟疾发病的地理信息系统,并在该软件的统计学扩展模块支持下,利用Kriging法对已建立的疟疾地理信息系统数据库进行空间插值分析,根据无偏最优的原则绘制疟疾发病概率的空间分布图,建立半变异函数,并对预测值的标准误差的分布制图。结果2005年黄淮地区疟疾发病分布呈空间自相关,自相关阈值98 928 m,其变异函数为球形模型,显示疟疾发病分布呈空间聚集性。交叉检验显示Krig-ing法生成的疟疾概率分布图是对黄淮地区疟疾空间分布的最优无偏估计,标准化均值为0.008 621。结论Kriging法能较好估计黄淮地区疟疾的空间分布特征,该地区的疟疾在空间分布上与距离有关,并呈现以安徽省中北部与河南交界及河南省与湖北省交界的两个明显的聚集中心,而且其空间分布并非与行政区划分类完全一致。 展开更多
关键词 黄淮地区 疟疾 地理信息系统 KRIGING
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西藏自治区消除疟疾的机遇、挑战与对策 被引量:8
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作者 周水森 严俊 +4 位作者 夏志贵 丰俊 胡桃 汤林华 周晓农 《中国寄生虫学与寄生虫病杂志》 CAS CSCD 北大核心 2015年第6期425-428,共4页
本文对西藏自治区疟疾流行特征及影响因素,实现消除疟疾目标面临的机遇与挑战,以及疟疾防控对策与建议进行评述。
关键词 消除疟疾 西藏自治区 机遇 挑战
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疟疾监测效果评价的新方法──综合指标的建立及其应用 被引量:7
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作者 周水森 汤林华 +2 位作者 钱会霖 郑香 罗曼珍 《中国寄生虫学与寄生虫病杂志》 CAS CSCD 北大核心 1998年第2期97-100,共4页
目的:建立评价疟疾监测效果的综合指标。方法:用德尔菲(Delphi)法由专家对常用效果评价指标进行筛选,以确定主要效果评价指标的权重,进而建立疟疾监测效果指数的计算式。结果:通过专家咨询方式确定主要效果指标的权重(W... 目的:建立评价疟疾监测效果的综合指标。方法:用德尔菲(Delphi)法由专家对常用效果评价指标进行筛选,以确定主要效果评价指标的权重,进而建立疟疾监测效果指数的计算式。结果:通过专家咨询方式确定主要效果指标的权重(W),分别为:当地感染的发病率为0.36、病人检出比例为0.31和输入继发病例/输入病例为0.33。得出疟疾监测效果指数(MSEI)=Σni=1Xi·Wi。按此式对甲、乙两种方案监测试点进行费用-效果分析,计算所得的得分分别为48.56和45.93,以致甲方案每取得1分约需乙方案所需费用的4倍。结论:此疟疾监测效果指数较为实际地反映疟疾监测方案的总体效果,适合于不同监测方案费用-效果评价。 展开更多
关键词 德尔菲法 疟疾 监测效果指数 费用效果分析
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ARIMA模型在疟疾发病预测中的应用 被引量:27
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作者 周水森 黄芳 沈毓祖 《中国病原生物学杂志》 CSCD 2007年第4期284-286,共3页
目的探讨ARIMA时间序列模型在疟疾发病预测中的应用,建立疟疾发病率的预测模型。方法基于1996~2005年安徽省怀远县的疟疾月发病资料,采用最大似然法估计模型参数,按照残差不相关原则、简洁原则确定模型结构,依据AIC与SBC准则确定模型... 目的探讨ARIMA时间序列模型在疟疾发病预测中的应用,建立疟疾发病率的预测模型。方法基于1996~2005年安徽省怀远县的疟疾月发病资料,采用最大似然法估计模型参数,按照残差不相关原则、简洁原则确定模型结构,依据AIC与SBC准则确定模型的阶数,建立ARIMA疟疾预测模型。并用所得模型对2006年该县疟疾发病率进行预测。结果ARIMA(0,1,1)×(0,1,1)12模型能较好地拟合既往时间段上的发病率时间序列,其方差估计值为0.60,AIC=187.00,SBC=193.58,数学函数式为(1-B)(1-B12)Zt=(1-0.591B)(1-0.281B12)at。该模型对2006年月发病率的平均预测误差仅为0.03。结论ARIMA模型可较好地拟合疟疾发病在时间序列上的变化趋势,是一种精度较高的短期预测模型。 展开更多
关键词 ARIMA模型 疟疾 预测
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核糖体28S-D3等位基因特异扩增鉴别微小按蚊亲缘种A和C 被引量:4
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作者 周水森 汤林华 +1 位作者 夏明仪 薛海筹 《中国媒介生物学及控制杂志》 CAS CSCD 2003年第6期408-410,共3页
目的 建立微小按蚊复合体不同亲缘种A和C的分子鉴别方法。方法 用单蚊蚊腿消化提取基因组DNA ,PCR特异扩增 2 8S第 3结构域 (D3 )基因 ,对PCR产物进行纯化、克隆、测序和序列分析 ;基于序列差异设计特异引物 ,进行等位基因特异扩增 (P... 目的 建立微小按蚊复合体不同亲缘种A和C的分子鉴别方法。方法 用单蚊蚊腿消化提取基因组DNA ,PCR特异扩增 2 8S第 3结构域 (D3 )基因 ,对PCR产物进行纯化、克隆、测序和序列分析 ;基于序列差异设计特异引物 ,进行等位基因特异扩增 (PCR -ASA) ,根据扩增片段大小区分微小按蚊不同亲缘种。结果 发现微小按蚊不同亲缘种A和C(GeneBank登录号 :AF416782 ,AF42 5 5 94) ,根据二者序列差异设计的特异引物能在普通琼脂糖凝胶上直观地区分两亲缘种 ,两者除在 3 76bp处有一共同条带外 ,分别在 2 94bp和 112bp处出现各自的特异扩增带。结论 我们建立的ASA分子鉴别方法能有效地将我国微小按蚊不同亲缘种A和C区分开来。 展开更多
关键词 微小按蚊 亲缘种 基因D3 分子鉴别 核糖体 等位基因
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微小按蚊种团mtDNA-COII基因变异的研究 被引量:4
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作者 周水森 汤林华 郑香 《中国寄生虫学与寄生虫病杂志》 CAS CSCD 北大核心 2002年第3期168-170,共3页
目的 研究我国微小按蚊种团成员 (微小按蚊A/C、乌头按蚊、瓦容按蚊及杰普尔按蚊 )的线粒体DNA(mtDNA)COII基因的分子变异和种系发生关系。 方法 单蚊提取DNA ;mtDNA COII基因的扩增和测序 ;用最大似然法DNAML构建种系发生树。 结... 目的 研究我国微小按蚊种团成员 (微小按蚊A/C、乌头按蚊、瓦容按蚊及杰普尔按蚊 )的线粒体DNA(mtDNA)COII基因的分子变异和种系发生关系。 方法 单蚊提取DNA ;mtDNA COII基因的扩增和测序 ;用最大似然法DNAML构建种系发生树。 结果与结论 我国存在微小按蚊A、C两个亲缘种 ,两者COII基因 1 6个位点存在碱基置换 ,突变频率为 2 .3 % 。 展开更多
关键词 基因变异 微小按蚊种团 线粒体DNA 种系发生 疟疾
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PCR-RFLP鉴别微小按蚊亲缘种A和C 被引量:5
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作者 周水森 汤林华 +1 位作者 薛海筹 夏明仪 《中国寄生虫病防治杂志》 CSCD 2003年第6期332-334,共3页
目的 建立微小按蚊复合体不同亲缘种A和C的分子鉴别方法—PCR产物酶切片段长度多态性分析 (PCR RFLP)。 方法 用单蚊蚊腿消化提取基因组DNA ,PCR特异扩增核糖体 2 8S第 3结构域 (D3 )基因 ,对PCR产物进行纯化、克隆和测序 ;分析微小... 目的 建立微小按蚊复合体不同亲缘种A和C的分子鉴别方法—PCR产物酶切片段长度多态性分析 (PCR RFLP)。 方法 用单蚊蚊腿消化提取基因组DNA ,PCR特异扩增核糖体 2 8S第 3结构域 (D3 )基因 ,对PCR产物进行纯化、克隆和测序 ;分析微小按蚊A和CD3基因的酶切图谱 ,选择合适的限制性内切酶对两者PCR产物进行特异性切割 ,按照酶切片段长度区分两亲缘种。 结果 微小按蚊A被限制性内切酶MboII切割后在约 3 76bp、2 68bp和 10 8bp处出现 3条条带 ,而微小按蚊C因第 96bp、97bp位点突变 ,不存在限制性内切酶MboII的特异识别位点而未被消化 ,仅在 3 76bp处存在唯一条带。  结论 本实验建立的PCR RFLP分子鉴别方法能有效地将形态难以鉴别的微小按蚊亲缘种A和C(GenBank :AF416782 ,AF415 5 94)区分开来。 展开更多
关键词 微小按蚊 亲缘种 D3基因 PCR-RFLP 蚊虫
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进化速率不同的分子标记对微小按蚊新亲缘种的研究 被引量:1
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作者 周水森 汤林华 《中国病原生物学杂志》 CSCD 2006年第6期416-419,共4页
目的选择进化速率不同的核糖体rDNA-ITS2r、DNA-28S-D3基因和线粒体DNA(mtDNA)COⅡ基因作为分子标记,研究我国微小按蚊变异及新的亲缘种。方法用蛋白酶K消化单蚊蚊腿,提取基因组DNA,PCR特异扩增rDNA-ITS2r、DNA-28S-D3和mtDNA-COⅡ基因... 目的选择进化速率不同的核糖体rDNA-ITS2r、DNA-28S-D3基因和线粒体DNA(mtDNA)COⅡ基因作为分子标记,研究我国微小按蚊变异及新的亲缘种。方法用蛋白酶K消化单蚊蚊腿,提取基因组DNA,PCR特异扩增rDNA-ITS2r、DNA-28S-D3和mtDNA-COⅡ基因片段,对PCR产物进行纯化、测序和序列分析,并基于mtDNA-COⅡ基因采用最大似然法构建种系发生树,分析微小按蚊变异地位和进化关系。结果1)云南元江微小按蚊rDNA-ITS2扩增片段约700 bp,其他微小按蚊为480 bp左右;2)ITS2和D3序列分析显示3种单倍型,其中元江微小按蚊基因长度与碱基组成显著不同;3)mtDNA-COⅡ种系发生树显示元江微小按蚊与其他微小按蚊的亲缘关系较远。结论我国存在微小按蚊A和C之外的新的微小按蚊亲缘种。 展开更多
关键词 微小按蚊 亲缘种 DNA分子标记
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微小按蚊复合体研究进展 被引量:1
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作者 周水森 《国外医学(寄生虫病分册)》 2002年第1期8-13,共6页
摘要 近年来,有关微小按蚊复合体的研究相继开展,尽管现代分子生物学技术在按蚊复合体研究中日渐重要,但传统的生物学方法在微小按蚊复合体近缘种鉴别研究中仍占主导地位。本文从以上两方面对其研究进展进行综述。
关键词 微小按蚊复合体 传统生物学 分子生物学 研究进展
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全国疟疾防治技术方案培训及研讨会在南宁召开
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作者 周水森 黄勇 《中国病原生物学杂志》 CSCD 2007年第3期167-167,共1页
关键词 疟疾防治 中国疾病预防控制中心 寄生虫病预防控制所 南宁 培训 专业技术人员 寄生虫病防治 卫生部
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2011年全国疟疾疫情分析 被引量:145
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作者 夏志贵 杨曼尼 周水森 《中国寄生虫学与寄生虫病杂志》 CAS CSCD 北大核心 2012年第6期419-422,共4页
本文根据2011年27个省(市、区)疾病预防控制中心或寄生虫病防治研究所上报的《疟疾防治工作调查表》(全国疾病控制调查制度2010年版“卫统29表”)汇总整理,未统计香港、澳门和台湾地区。
关键词 疟疾防治 疫情分析 疾病预防控制中心 调查制度 疾病控制 寄生虫病 台湾地区 调查表
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