Recently, plasma sterilization has attracted increasing attention in dental community ibr the atmospheric pressure non-equilibrium plasma jet (APNPs), which is driven by a kilohertz pulsed DC power, may be applied t...Recently, plasma sterilization has attracted increasing attention in dental community ibr the atmospheric pressure non-equilibrium plasma jet (APNPs), which is driven by a kilohertz pulsed DC power, may be applied to the dental and oral diseases. However, it is still in doubt whether APNPs can effectively kill pathogenic bacteria in the oral cavity and produce no harmful effects on normal oral tissues, especially on normal mucosa. The aim of this study was to evaluate the bacterial-killing effect of APNPs in the biofilms containing a single breed of bacteria (Porphyromonas gingivalis, Pg.), and the pathological changes of the oral mucosa after treatment by APNPs. P.g. was incubated to form the biofilms in vitro, and the samples were divided into three groups randomly: group A (blank control); group B in which the biofilms were treated by APNPs (the setting of the equipment: 10 kHz, 1600 ns and 8 kV); group C in which the biofilms were exposed only to a gas jet without ignition of the plasma. Each group had three samples and each sample was processed for up to 5 min. The biofilms were then fluorescently stained, observed and photographed under a laser scanning confocal microscope. In the animal experiment, six male Japanese white rabbits were divided into two groups randomly (n=3 in each group) in terms of the different post-treatment time (1-day group and 5-day group). The buccal mucosa of the left side and the mucosa of the ventral surface of the tongue were treated by APNPs for 10 min in the same way as the bacterial biofilm experiment in each rabbit, and the corresponding mucosa of the other sides served as normal control. The clinical manifestations of the oral mucosa were observed and recorded every day. The rabbits were sacrificed one or five day(s) after APNPs treatment. The oral mucosa were harvested and prepared to haematoxylin and eosin-stained sections. Clinical observation and histopathological scores were used to assess mucosal changes. The results showed the obvious P.g. biofilms were formed at 10 days, and most of the bacteria in groups A and C were alive under a laser scanning confocal microscope, but the bacteria in the group B were almost all dead. In animal experiment, no ulcers, anabrosis and oral mucositis were found in both the 1-day and 5-day groups. The aver- age mucous membrane irritation index was -0.83 and -0.67 in the 1-day and 5-day groups, respectively, suggesting that no intense mucosal membrane irritation responses occurred. It was concluded that APNPs could effectively kill Pg. in the biofilms and did not cause any pathological changes in the normal mucosa, suggesting that the plasma jet (APNPs) may be applied to oral diseases as a novel sterilization device in the future.展开更多
目的:探讨口腔卫生维护对2型糖尿病患者种植牙牙周情况和糖化血红蛋白的影响。方法:将113名种植牙患者分为三组,第一组38名,为未患糖尿病者,糖化血红蛋白值<6%;第二组36名,为2型糖尿病患者,糖化血红蛋白值在6.1%~8.0%之间;第三组39名...目的:探讨口腔卫生维护对2型糖尿病患者种植牙牙周情况和糖化血红蛋白的影响。方法:将113名种植牙患者分为三组,第一组38名,为未患糖尿病者,糖化血红蛋白值<6%;第二组36名,为2型糖尿病患者,糖化血红蛋白值在6.1%~8.0%之间;第三组39名,为2型糖尿病患者,糖化血红蛋白值在8.1%~10.0%之间。三组患者均种植牙后即刻负重,在术后每6个月进行牙周/种植体周洁治;分别在术后6个月、1年和2年行龈沟出血指数(sulcus bleeding index,SBI)、探诊深度(probing depth,PD)和骨丧失深度(the distance from the shoulder of the implant to the bottom of the bony defect,DSB)检测。结果:第一、二、三组术前的平均糖化血红蛋白值分别为4.85%、6.88%和8.64%;在第一组,术前、术后6个月、术后1年和2年的糖化血红蛋白值之间的变化无统计学差异(P>0.05);在第二组和第三组,术后2年和术后6个月之间的糖化血红蛋白值显著下降(P<0.01);在术后6个月,第一组的SBI、PD和DSB低于第三组,差异有统计学意义(P<0.01);在术后1年和2年,观察二组的DSB均显著性高于第三组(P<0.01);第三组的SBI和PD在术后2年时较术后6个月时显著下降(P<0.01)。结论:口腔卫生维护可降低2型糖尿病人种植牙后的糖化血红蛋白值,同时也能缓解种植体周围的炎症症状。展开更多
基金supported by a grant from the National Natural Science Foundation of China(No.10875048)
文摘Recently, plasma sterilization has attracted increasing attention in dental community ibr the atmospheric pressure non-equilibrium plasma jet (APNPs), which is driven by a kilohertz pulsed DC power, may be applied to the dental and oral diseases. However, it is still in doubt whether APNPs can effectively kill pathogenic bacteria in the oral cavity and produce no harmful effects on normal oral tissues, especially on normal mucosa. The aim of this study was to evaluate the bacterial-killing effect of APNPs in the biofilms containing a single breed of bacteria (Porphyromonas gingivalis, Pg.), and the pathological changes of the oral mucosa after treatment by APNPs. P.g. was incubated to form the biofilms in vitro, and the samples were divided into three groups randomly: group A (blank control); group B in which the biofilms were treated by APNPs (the setting of the equipment: 10 kHz, 1600 ns and 8 kV); group C in which the biofilms were exposed only to a gas jet without ignition of the plasma. Each group had three samples and each sample was processed for up to 5 min. The biofilms were then fluorescently stained, observed and photographed under a laser scanning confocal microscope. In the animal experiment, six male Japanese white rabbits were divided into two groups randomly (n=3 in each group) in terms of the different post-treatment time (1-day group and 5-day group). The buccal mucosa of the left side and the mucosa of the ventral surface of the tongue were treated by APNPs for 10 min in the same way as the bacterial biofilm experiment in each rabbit, and the corresponding mucosa of the other sides served as normal control. The clinical manifestations of the oral mucosa were observed and recorded every day. The rabbits were sacrificed one or five day(s) after APNPs treatment. The oral mucosa were harvested and prepared to haematoxylin and eosin-stained sections. Clinical observation and histopathological scores were used to assess mucosal changes. The results showed the obvious P.g. biofilms were formed at 10 days, and most of the bacteria in groups A and C were alive under a laser scanning confocal microscope, but the bacteria in the group B were almost all dead. In animal experiment, no ulcers, anabrosis and oral mucositis were found in both the 1-day and 5-day groups. The aver- age mucous membrane irritation index was -0.83 and -0.67 in the 1-day and 5-day groups, respectively, suggesting that no intense mucosal membrane irritation responses occurred. It was concluded that APNPs could effectively kill Pg. in the biofilms and did not cause any pathological changes in the normal mucosa, suggesting that the plasma jet (APNPs) may be applied to oral diseases as a novel sterilization device in the future.
文摘目的:探讨口腔卫生维护对2型糖尿病患者种植牙牙周情况和糖化血红蛋白的影响。方法:将113名种植牙患者分为三组,第一组38名,为未患糖尿病者,糖化血红蛋白值<6%;第二组36名,为2型糖尿病患者,糖化血红蛋白值在6.1%~8.0%之间;第三组39名,为2型糖尿病患者,糖化血红蛋白值在8.1%~10.0%之间。三组患者均种植牙后即刻负重,在术后每6个月进行牙周/种植体周洁治;分别在术后6个月、1年和2年行龈沟出血指数(sulcus bleeding index,SBI)、探诊深度(probing depth,PD)和骨丧失深度(the distance from the shoulder of the implant to the bottom of the bony defect,DSB)检测。结果:第一、二、三组术前的平均糖化血红蛋白值分别为4.85%、6.88%和8.64%;在第一组,术前、术后6个月、术后1年和2年的糖化血红蛋白值之间的变化无统计学差异(P>0.05);在第二组和第三组,术后2年和术后6个月之间的糖化血红蛋白值显著下降(P<0.01);在术后6个月,第一组的SBI、PD和DSB低于第三组,差异有统计学意义(P<0.01);在术后1年和2年,观察二组的DSB均显著性高于第三组(P<0.01);第三组的SBI和PD在术后2年时较术后6个月时显著下降(P<0.01)。结论:口腔卫生维护可降低2型糖尿病人种植牙后的糖化血红蛋白值,同时也能缓解种植体周围的炎症症状。