One bacteria endophyte were isolated from the root nodules of Podocarpus macrophyllus(Thunb.)D.Don.The bacteria has the ordinary characteristics of Rhizobium of leguminous plants after indentification.It made nodulati...One bacteria endophyte were isolated from the root nodules of Podocarpus macrophyllus(Thunb.)D.Don.The bacteria has the ordinary characteristics of Rhizobium of leguminous plants after indentification.It made nodulation on its host after inoculation strains.So did on Podocarpus nagi(Thunb.)zoll.et Mor.ex Zoll. seedling which is of the same family and genus with Podocarpus macrophyllus(Thunb.)D.Don.Both strain and host seeding have C 2H 4 reduction activity.But it can not made nodulation on 11 kind of offering tests of leguminous plants after inoculation strains.According to its characteristics the bacteria thus appears to be a member of a disinct Rhizobium lineage.Its phylogenetic relationship position remain to be research.展开更多
细菌的σ54(R poN)是一类可选择性识别启动子序列的σ因子,主要参与环境适应、细胞生理过程等,如氮代谢、鞭毛和菌毛的生物合成。在一些病原菌中,σ54也与致病性密切有关。为了明确σ54在野油菜黄单胞菌野油菜致病变种(X cc)中是否参与...细菌的σ54(R poN)是一类可选择性识别启动子序列的σ因子,主要参与环境适应、细胞生理过程等,如氮代谢、鞭毛和菌毛的生物合成。在一些病原菌中,σ54也与致病性密切有关。为了明确σ54在野油菜黄单胞菌野油菜致病变种(X cc)中是否参与致病过程,用同源单交换定点突变方法,将X cc 8004中的两个σ54编码基因rp oN 1(XC 1256)和rp oN 2(XC 2168)做单突变及双突变。突变体表型分析结果表明,单个和同时突变rp oN 1和rp oN 2基因均不影响野油菜黄单胞菌的正常生长,对胞外多糖(EPS)产生、胞外酶的合成也无明显影响。植株致病性检测结果表明,rp oN 1和rp oN 2基因的单突变或双突变体的致病力与野生型菌株没有显著差异,表明野油菜黄单胞菌野油菜致病变种的致病过程不需要σ54因子参与。展开更多
文摘One bacteria endophyte were isolated from the root nodules of Podocarpus macrophyllus(Thunb.)D.Don.The bacteria has the ordinary characteristics of Rhizobium of leguminous plants after indentification.It made nodulation on its host after inoculation strains.So did on Podocarpus nagi(Thunb.)zoll.et Mor.ex Zoll. seedling which is of the same family and genus with Podocarpus macrophyllus(Thunb.)D.Don.Both strain and host seeding have C 2H 4 reduction activity.But it can not made nodulation on 11 kind of offering tests of leguminous plants after inoculation strains.According to its characteristics the bacteria thus appears to be a member of a disinct Rhizobium lineage.Its phylogenetic relationship position remain to be research.
文摘细菌的σ54(R poN)是一类可选择性识别启动子序列的σ因子,主要参与环境适应、细胞生理过程等,如氮代谢、鞭毛和菌毛的生物合成。在一些病原菌中,σ54也与致病性密切有关。为了明确σ54在野油菜黄单胞菌野油菜致病变种(X cc)中是否参与致病过程,用同源单交换定点突变方法,将X cc 8004中的两个σ54编码基因rp oN 1(XC 1256)和rp oN 2(XC 2168)做单突变及双突变。突变体表型分析结果表明,单个和同时突变rp oN 1和rp oN 2基因均不影响野油菜黄单胞菌的正常生长,对胞外多糖(EPS)产生、胞外酶的合成也无明显影响。植株致病性检测结果表明,rp oN 1和rp oN 2基因的单突变或双突变体的致病力与野生型菌株没有显著差异,表明野油菜黄单胞菌野油菜致病变种的致病过程不需要σ54因子参与。