The CRISPR-Cas9 system,serving as a powerful genome-editing technology,has revolutionized the life sciences.However,it exhibits off-target activities that may present severe problems in clinical applications.Although ...The CRISPR-Cas9 system,serving as a powerful genome-editing technology,has revolutionized the life sciences.However,it exhibits off-target activities that may present severe problems in clinical applications.Although a great number of in silico models have been developed to predict CRISPR targeting efficiency and specificity,they are running into a bottleneck with the lack of a mechanistic understanding of the on-and off-target activities of Cas9.展开更多
基金supported by the National Key Research and Development Program of China(2021YFF1201200,2021YFF1200900)the National Natural Science Foundation of China(31970638,61572361,62102286,and 62002265)+4 种基金Roche pRED Informatics Advanced Analytics Postdoctoral Fellowship Program(aligned with the Roche pRED Postdoctoral Fellowship Program RPF-500)Shanghai Natural Science Foundation Program(17ZR1449400)Shanghai Artificial Intelligence Technology Standard Project(19DZ2200900)Shanghai Shuguang Scholars ProjectWe Bank Scholars Project and Fundamental Research Funds for the Central Universities。
文摘The CRISPR-Cas9 system,serving as a powerful genome-editing technology,has revolutionized the life sciences.However,it exhibits off-target activities that may present severe problems in clinical applications.Although a great number of in silico models have been developed to predict CRISPR targeting efficiency and specificity,they are running into a bottleneck with the lack of a mechanistic understanding of the on-and off-target activities of Cas9.
