复方树莓籽粉对辐照小鼠骨髓细胞的防护作用

探讨复方树莓籽粉水溶液对急性放射性损伤小鼠骨髓细胞的影响及防护作用。建立小鼠急性放射性损伤模型,设立空白对照组、模型对照组及不同浓度复方树莓籽粉溶液处理组(低、中、高剂量组),观察辐照后各组小鼠骨髓细胞有核细胞数、微核率、细胞周期及线粒体膜电位的变化情况。实验结果表明,与空白对照组比较,模型对照组变化均非常显著(p<0.01)。与模型对照组比较,复方树莓籽粉中、高剂量组有核细胞数分别升高78.2%(p<0.01)、116.9%(p<0.01);高剂量组微核率减少27.3%(p<0.01);中剂量组细胞周期G1期、S期,高剂量组S期细胞比例升高(p<0.05),高剂量组G1期细胞比例显著升高(p<0.01);中、高剂量组骨髓细胞线粒体膜电位显著升高(p<0.01)。实验结果提示,复方树莓籽粉能升高骨髓细胞数,降低骨髓细胞微核率,升高骨髓细胞线粒体膜电位,减少骨髓细胞周期阻滞,抑制辐射损伤骨髓细胞的细胞凋亡,可能对辐照导致的骨髓细胞损伤有防护作用。

从六经辨治新型冠状病毒肺炎

广义的伤寒是一切外感病的总称,温病亦属其中,六经辨证亦可运用于新型冠状病毒肺炎(corona virus disease 2019,COVID-19)各期的治疗中。预防期,以调节阴阳平衡,祛除湿邪为主,方用桂枝汤加茯苓、白术。素体痰湿较重者,方用防己黄芪汤加减。初期,病主在太阳或太阴。病在太阳,依证选用麻杏石甘汤加减、大青龙汤加减、桔梗汤等。病在太阴,方用理中汤加减。若太阳、太阴合病,方用桂枝去桂加茯苓白术汤加减。若太阳、少阴合病,方用麻黄细辛附子汤加减。中期,病主在少阳或阳明。病在少阳,方用小柴胡汤加减。病在阳明,依证选用白虎汤加减、小承气汤加减等。若少阳、阳明合病,方用大柴胡汤加减。若太阳、太阴合病,依证选用射干麻黄汤加减、葶苈大枣泻肺汤、小陷胸汤、麻黄连翘赤小豆汤加减等。若阳明、少阴合病,方用猪苓汤加减。极期,病主在阳明、少阴或厥阴。阳气暴脱证,可予参附注射液静脉滴注,气阴两虚证,可予生脉注射液静脉滴注。病在阳明,方用大承气汤加减。病在少阴,方用真武汤加减。病在厥阴,方用乌梅丸加减。恢复期,病主在阳明或太阴。病在阳明,方用竹叶石膏汤加减。病在太阴,方用参苓白术散加减。运用六经辨证可以了解疾病的发生发展过程,然疾病之传变方式多样,不可一概而论,辨证施治仍为第一要义。

针刺远近取穴对痛证疗效影响的Meta分析

目的:评价针刺远近穴位对痛证疗效的影响。方法:根据循证医学(EBM)及系统评价相关方法,对近20年国内外常用数据库针刺远近穴位治疗痛证的文献进行评价研究。结果:纳入研究11个患者653例,Meta分析显示远近取穴针刺1次,疼痛疗效比较PPI量表和VAS量表,2组间差异有统计学意义,表明针刺1次远端取穴疗效优于近端取穴,远近取穴针刺1个疗程、2个疗程和2个疗程以上时2组间比较差异无统计学意义,表明针刺1个疗程或更长时间远近取穴疗效相当。结论:快速镇痛应选择针刺远端穴位,需长期镇痛治疗时针刺远近端穴位疗效相差不大。

Meta analysis of the effect of distal or local point selection on acupuncture efficacy

To evaluate the effect of distal point selection, local point selection, and distal-local point selection on acupuncture efficacy. Methods： According to the requirements of evidence-based medicine（EBM）, the literature on acupuncture with distal and local point selection in recent 20 years included in domestic and overseas medical databases was retrieved, and meta-analysis of the included articles was carried out. Results： Thirty-two articles were included finally, totaling 2829 patients. The results of meta-analysis indicated that the curative effect of distal-local point combination was superior to that of simple distal point selection or local point selection during acupuncture–moxibustion treatment for diseases, and there was no obvious difference in the curative effect between simple distal point selection and local point selection. According to the comprehensive analysis of 18 included articles, the difference in total effective rate between distal point selection and local point selection was not statistically significant, i.e. the curative effect of distal point selection and local point selection was equivalent [OR=0.83,95%CI（0.83,1.18）, Z=1.04, P0.01]. According to the comprehensive analysis of 20 included articles, the difference in total effective rate between local point selection and distal-local point selection was statistically significant, and the results indicated that the curative effect of distal-local point selection was superior to that of local point selection [OR=0.32, 95%CI（0.23,0.44）, Z=6.90, P0.01]. According to the comprehensive analysis of 8 included articles, the difference in total effective rate between distal point selection and distal-local point selection was statistically significant, and the results indicated that the curative effect of distal-local point selection was superior to that of distal point selection [OR=0.20,95%CI（0.10,0.40）, Z=4.50, P0.01]. In addition, the analysis of publication bias of this study indicated that publication bias might exist. Since the quality of included articles was generally lower, the above conclusion still needed to be supported by more evidence-based medicine proofs with high quality. Conclusion： The curative effect of distal-local point combination was superior to that of simple distal point selection or local point selection during acupuncture treatment for diseases, and there was no obvious difference in the curative effect between simple distal point selection and local point selection.

Piwil2及Stat3、Bcl-2蛋白在雄性不育小鼠睾丸组织中表达?

本研究主要是探讨Piwil2、Stat3、Bcl-2蛋白在不育的雄性小鼠中的表达量及三者之间的表达位置相关性。取雄性昆明种小鼠60只,随机分为实验组与对照组,每组30只。实验组采用雷公藤多苷药物灌胃造小鼠不育模型28 d;对照组按照同样剂量的生理盐水进行灌胃,持续时间及频次同实验组。造模后将两组雄性小鼠分别与雌性小鼠交配;再处死雄性小鼠取出两组的睾丸组织,采用免疫组织化学染色法和蛋白印迹检测法分别检测样本中Piwil2、Stat3及Bcl-2蛋白的表达状况。将实验组与对照组的检测结果进行比较,观察两组的蛋白表达差异性及三个蛋白表达的相关性。H.E染色显示,实验组小鼠睾丸组织生精小管结构与对照组相比,明显被破坏,精原细胞及初次级精母细胞数量明显减少,结合与雌鼠交配后受精能力明显下降的结果,说明不育造模成功。免疫组化(IHC)染色结果显示,实验组Piwil2、Stat3及Bcl-2蛋白的染色程度及阳性细胞数均明显低于对照组(P <0.01)。Western Blot结果同样显示,三种蛋白在实验组的表达量明显低于对照组(Piwil2蛋白P <0.05,Stat3蛋白P <0.05,Bcl-2蛋白P <0.01)。本研究说明,Piwil2、Stat3及Bcl-2蛋白在雄性不育小鼠中表达量均显著降低,这三个蛋白对小鼠精子生成及小鼠不育的发生起到重要调节作用。