肾俞穴延缓衰老的应用与研究

目的:探讨肾俞穴在延缓衰老中的作用。方法:从中医对肾虚、衰老的认识、肾俞穴概况及肾俞穴延缓衰老的应用等方面加以论述。结论:肾俞穴是延缓衰老的要穴,肾俞穴益精补肾疗法对于养生长寿有着广阔的前景。

艾灸对老年人衰老症状及外周血单个核细胞细胞周期的影响

目的:探讨艾灸延缓衰老的作用及对细胞周期的影响。方法:将61名60～75岁老年人随机分为艾灸治疗组和衰老对照组,并另设正常青年人对照组。艾灸组以温和灸肾俞和关元穴,疗程3个月。采用积分表法观察艾灸对老年人衰老症状的变化情况,用流式细胞术检测老年人外周血单核细胞细胞周期变化。结果:艾灸组较衰老对照组衰老症状积分明显降低(P<0.01),综合疗效、主症有效率均明显升高(P<0.01);G0/G1期细胞比例明显减少(P<0.01),PI指数显著升高(P<0.01)。结论:提示艾灸不仅可明显改善老年人衰老症状,且可能通过降低细胞周期G0/G1期细胞比例,升高PI指数,增加有丝分裂原的反应能力和合成DNA的能力,使细胞进入S期,从而发挥延缓细胞衰老的作用。

温和灸对自然衰老模型大鼠肝组织Rb、P53、Bcl-2、PKC表达的影响

目的研究温和灸对自然衰老大鼠肝组织Rb、P53、Bcl-2、PKC蛋白及其mRNA表达的影响。方法 24只SD大鼠随机分为正常组、自然衰老组、艾灸组。正常饲养20个月,待其自然进入衰老期以制备自然衰老大鼠模型,温和灸自然衰老大鼠双侧肾俞穴对其进行干预。应用原位杂交、免疫组化方法检测各组大鼠肝组织Rb、P53、Bcl-2、PKCmRNA及其蛋白表达。结果与正常组比较,自然衰老组大鼠肝组织Rb、P53mRNA及蛋白表达均显著增强(P<0.01,P<0.05),Bcl-2、PKCmRNA及蛋白表达均显著降低(P<0.01,P<0.05);与自然衰老组大鼠比较,艾灸组大鼠肝组织Rb、P53mRNA及蛋白表达均显著降低(P<0.01,P<0.05),Bcl-2、PKCmRNA及蛋白表达均显著增强(P<0.01,P<0.05)。结论温和灸肾俞穴能降低自然衰老大鼠肝组织Rb、P53mR-NA及蛋白表达,增强Bcl-2、PKCmRNA及蛋白表达。

按时取穴针灸治疗失眠45例临床观察

目的:观察按时取穴针灸治疗失眠的临床疗效。方法:90名失眠患者分为治疗组和对照组,治疗组针刺与走罐治疗时间为下午3～5点,并按时点按涌泉、劳宫穴,连续治疗3个月。对照组针刺与走罐治疗时间在上午,点按穴位时间不固定。以匹兹堡睡眠质量指数(PSQI)评分进行疗效评估,观察2组临床疗效及治疗前后PSQI评分变化。结果:治疗组总有效率、痊愈率均明显优于对照组,愈显率也优于对照组;治疗组治疗后PSQI总积分及差值改善均优于对照组;治疗后3个月随访,治疗组总有效率、痊愈率、愈显率均明显优于对照组。结论:按时取穴针灸治疗失眠的疗效优于常规针灸治疗。

温针灸对老年人微量元素的影响

目的:观察温针灸延缓衰老的疗效及对老年人微量元素的影响。方法:以100名老年前期及老年期受试者作为观察对象,随机分为治疗组及衰老对照组,并另设正常青年对照组。温针灸足三里、关元;脾俞、肾俞,连续治疗3个月。用积分表法观察治疗后积分变化及疗效情况;用原子吸收分光光度法检测血中锌、锰、硒含量的变化。结果:治疗组老年前期、老年期疗效及积分变化均有统计学意义(P<0.01),且老年前期疗效优于老年期(P<0.05)。治疗组血中锌含量较对照组升高(P<0.05),锰、硒含量亦较对照组升高(P<0.01)。结论:温针灸能明显改善衰老症状,老年前期疗效优于老年期,温针灸可能通过调节体内微量元素含量来延缓衰老。

温针灸延缓衰老的临床观察

目的:观察温针灸延缓衰老的临床疗效。方法:以100名受试者作为观察对象,随机分为2组,即治疗组和对照组,温针灸足三里、关元、脾俞、肾俞,连续治疗3个月。用积分表法观察治疗后症状积分变化及疗效情况。结果:治疗组综合疗效、主症疗效均显著高于对照组(P<0.01);治疗后衰老症状积分、主症积分均明显低于对照组(P<0.01);脾阳虚型有效率稍优于肾阳虚型,但差异无统计学意义(P>0.05)。结论:温针灸能明显改善衰老症状,延缓衰老。

端粒和端粒酶及其与衰老的关系

细胞衰老是指正常细胞的有限分裂增殖性，DNA的不断复制和细胞分裂伴随着端粒长度的逐渐缩短及结构功能的改变，端粒长度、结构及功能依赖端粒酶活性的调节。深入地剖析端粒、端粒酶及与衰老的关系，将可能会更快地揭示衰老的机制。

Effects of mild-warming moxibustion on Bcl-2 and PKC expressions of peripheral blood in elderly people

OBJECTIVE:To explore the anti-aging effects of mild-warming moxibustion on Bcl-2 and PKC expression in peripheral blood and general symptoms in elderly people.METHODS:A total of 61 elderly people and 30 non-elderly people were enrolled.The total effective rate of mild-warming moxibustion was assessed by symptom scores,and Bcl-2 and PKC expression in peripheral blood was detected by flow cytometry.RESULTS:The total effective rate in the mild-warming moxibustion group was significantly higher than in the blank control group(P<0.01).Bcl-2 and PKC expression rates in peripheral blood in the blank control group were lower than in the normal control group(P<0.01),but higher after mild-warming moxibustion(P<0.01).CONCLUSION:The anti-aging effects of mild-warm-ing moxibustion may be due to increased Bcl-2 and PKC expression in peripheral blood in aged people.

Moxibustion on Telomerase Activity in Aging Rat

Objective： To investigate the effect of moxibustion on telomerase activity and genes expression in tissues of senescent rats. Methods： Subacute aging rats model were established by injection with D-gal solution. Points Shenshu （BL 23） were treated with moxibustion in treatment group, contrasting with a model group and a normal group. Enzyme-Linked Immunosorbent Assays（ELISA） was used for the level of telomerase activity in liver tissues, and In Situ Hybridization（ISH） was used for the condition of expression of telomerase genes in liver tissues. Results： The level of telomerase activity in the aging model group was obviously lower than that in normal control group （P〈0.01）, the level in moxibustion group was obviously higher than that in model group（P〈0.05）. In comparison with normal rats, the positive-expressed areas and photodensity of telomerase genes in aging model group were all significantly lower （P〈0.01, P〈0.01）, and the positive-expressed areas in moxibustion group was significantly higher than that in model group （P〈0.01）. Conclusions： Moxibustion could regulate telomerase activity of senile rats, hence delaying aging.