以极大螺旋藻作为实验材料 ,研究了不同 CO2 浓度培养对螺旋藻光抑制和恢复的影响 ,结果表明由光抑制导致的光合速率下降 ,高浓度 CO2 比低浓度 CO2 培养程度小 ,在高浓度 CO2 条件下培养的极大螺旋藻 ,虽然在强光下也表现出光抑制 ,但...以极大螺旋藻作为实验材料 ,研究了不同 CO2 浓度培养对螺旋藻光抑制和恢复的影响 ,结果表明由光抑制导致的光合速率下降 ,高浓度 CO2 比低浓度 CO2 培养程度小 ,在高浓度 CO2 条件下培养的极大螺旋藻 ,虽然在强光下也表现出光抑制 ,但与低浓度 CO2 相比 ,光合速率下降得较慢。这种现象在强光与弱光培养均存在 ,但强光培养时更明显。光抑制后的恢复实验表明 ,不同 CO2 浓度培养的极大螺旋藻 ,光系统 光化学活性 (Fv/Fm)在弱光下恢复较好 ,高光强、高浓度 CO2 培养的藻 ,恢复速度稍快 ;而在黑暗中 ,几乎没有恢复 ;在弱光和含氯霉素的条件下 Fv/Fm均下降。由此可见 ,高 CO2 浓度可减轻极大螺旋藻的光抑制 ,但对其光抑制后的恢复影响不大。展开更多
Bangia fusco-purpurea,an economic macroalgae,was often cultivated in Zhejiang and Fujian coastal areas.Its cultivation in Guangdong coast and photosynthetic characteristics had not been well documented.In this paper i...Bangia fusco-purpurea,an economic macroalgae,was often cultivated in Zhejiang and Fujian coastal areas.Its cultivation in Guangdong coast and photosynthetic characteristics had not been well documented.In this paper it was transplanted from Fujian Province to the sea area of Nan′ao Island in Guangdong Province,and the photosynthetic characteristics and fatty acids compoment were investigated by using photosynthetic gas exchange and gas chromatography.The results showed that Bangia fusco-purpurea grew well in situ with a higher photosynthetic light saturation point of 210 μmol/(m2·s).The lower light compensation point as well as dark respiration rate were also observed.The polyunsaturated fatty acids(PUFAs) accounted for more than half of the total fatty acids.The eicosapentaenoic acid(EPA) content was 41.20% in the total fatty acids,and reached as high as 499.82 mg per 100 g dry weight,which was significantly higher than that of Porphyra haitanensis or Gracilaria lemaneiformis grown in the same sea area.In conclusion,it was practicable that Bangia fusco-purpurea was cultivated in the sea area of Nan′ao Island.The algae could be exploited as a new source of EPA production because of its higher EPA content.展开更多
Elevated CO2 concentration had insignificant effect on shape and size of Chlorella pyrenoidosa, but increased the amount of Chloroplast. Pyrenoids were found in low-CO2-grown cells, but not in high-CO2-grown cells. Th...Elevated CO2 concentration had insignificant effect on shape and size of Chlorella pyrenoidosa, but increased the amount of Chloroplast. Pyrenoids were found in low-CO2-grown cells, but not in high-CO2-grown cells. The chloroplast lamella of low-CO2-grown cells had higher electrical density than that of high-CO2-grown cells.展开更多
order to investigate the morphological response of freshwater green algae to elevated CO2 concentration, Chlamydomonas reinhardtii Dang and Scenedesmus obliquus Kutz were cultured with enriched CO2, and their microstr...order to investigate the morphological response of freshwater green algae to elevated CO2 concentration, Chlamydomonas reinhardtii Dang and Scenedesmus obliquus Kutz were cultured with enriched CO2, and their microstructure and ultrastructure were examined by microscopy and electron microscopy. The effect of CO2 enrichment to 186 mumol/L, was insignificant on the shape and size of C. reinhardtii, but significant in reducing the volume of S. obliquus. High-CO2 increased the amount of chloroplast. The pyrenoids occurred in low-CO2-grown cells but not in high-CO2-grown ones and more starch granules were observed in the former.展开更多
以赤潮硅藻小新月菱形藻(Nitzschia closterium var. minutissima)为实验材料,研究了短期内(12h)高浓度CO2(5%CO2)对其胞外碳酸酐酶活性和光合作用的影响,结果显示,高浓度CO2培养导致小新月菱形藻胞外碳酸酐酶活性、叶绿素a和叶绿素c含...以赤潮硅藻小新月菱形藻(Nitzschia closterium var. minutissima)为实验材料,研究了短期内(12h)高浓度CO2(5%CO2)对其胞外碳酸酐酶活性和光合作用的影响,结果显示,高浓度CO2培养导致小新月菱形藻胞外碳酸酐酶活性、叶绿素a和叶绿素c含量明显下降.与通空气培养(0.035%CO2)相比,在短期内(12h)胞外碳酸酐酶活性下降了75.4%,叶绿素a、c含量分别降低了5.6%和7.3%;高浓度CO2培养下最大光化学效率(Fv/Fm)、实际光化学效率(Yield)和光化学淬灭系数(qP)下降,但非光化学淬灭系数(qN)升高.研究结果表明,高浓度CO2对胞外碳酸酐酶活性具有明显的抑制作用,小新月菱形藻通过调整光系统II的能量流动和能量利用效率以适应高浓度CO2的环境.展开更多
文摘以极大螺旋藻作为实验材料 ,研究了不同 CO2 浓度培养对螺旋藻光抑制和恢复的影响 ,结果表明由光抑制导致的光合速率下降 ,高浓度 CO2 比低浓度 CO2 培养程度小 ,在高浓度 CO2 条件下培养的极大螺旋藻 ,虽然在强光下也表现出光抑制 ,但与低浓度 CO2 相比 ,光合速率下降得较慢。这种现象在强光与弱光培养均存在 ,但强光培养时更明显。光抑制后的恢复实验表明 ,不同 CO2 浓度培养的极大螺旋藻 ,光系统 光化学活性 (Fv/Fm)在弱光下恢复较好 ,高光强、高浓度 CO2 培养的藻 ,恢复速度稍快 ;而在黑暗中 ,几乎没有恢复 ;在弱光和含氯霉素的条件下 Fv/Fm均下降。由此可见 ,高 CO2 浓度可减轻极大螺旋藻的光抑制 ,但对其光抑制后的恢复影响不大。
文摘Bangia fusco-purpurea,an economic macroalgae,was often cultivated in Zhejiang and Fujian coastal areas.Its cultivation in Guangdong coast and photosynthetic characteristics had not been well documented.In this paper it was transplanted from Fujian Province to the sea area of Nan′ao Island in Guangdong Province,and the photosynthetic characteristics and fatty acids compoment were investigated by using photosynthetic gas exchange and gas chromatography.The results showed that Bangia fusco-purpurea grew well in situ with a higher photosynthetic light saturation point of 210 μmol/(m2·s).The lower light compensation point as well as dark respiration rate were also observed.The polyunsaturated fatty acids(PUFAs) accounted for more than half of the total fatty acids.The eicosapentaenoic acid(EPA) content was 41.20% in the total fatty acids,and reached as high as 499.82 mg per 100 g dry weight,which was significantly higher than that of Porphyra haitanensis or Gracilaria lemaneiformis grown in the same sea area.In conclusion,it was practicable that Bangia fusco-purpurea was cultivated in the sea area of Nan′ao Island.The algae could be exploited as a new source of EPA production because of its higher EPA content.
文摘Elevated CO2 concentration had insignificant effect on shape and size of Chlorella pyrenoidosa, but increased the amount of Chloroplast. Pyrenoids were found in low-CO2-grown cells, but not in high-CO2-grown cells. The chloroplast lamella of low-CO2-grown cells had higher electrical density than that of high-CO2-grown cells.
文摘order to investigate the morphological response of freshwater green algae to elevated CO2 concentration, Chlamydomonas reinhardtii Dang and Scenedesmus obliquus Kutz were cultured with enriched CO2, and their microstructure and ultrastructure were examined by microscopy and electron microscopy. The effect of CO2 enrichment to 186 mumol/L, was insignificant on the shape and size of C. reinhardtii, but significant in reducing the volume of S. obliquus. High-CO2 increased the amount of chloroplast. The pyrenoids occurred in low-CO2-grown cells but not in high-CO2-grown ones and more starch granules were observed in the former.
文摘以赤潮硅藻小新月菱形藻(Nitzschia closterium var. minutissima)为实验材料,研究了短期内(12h)高浓度CO2(5%CO2)对其胞外碳酸酐酶活性和光合作用的影响,结果显示,高浓度CO2培养导致小新月菱形藻胞外碳酸酐酶活性、叶绿素a和叶绿素c含量明显下降.与通空气培养(0.035%CO2)相比,在短期内(12h)胞外碳酸酐酶活性下降了75.4%,叶绿素a、c含量分别降低了5.6%和7.3%;高浓度CO2培养下最大光化学效率(Fv/Fm)、实际光化学效率(Yield)和光化学淬灭系数(qP)下降,但非光化学淬灭系数(qN)升高.研究结果表明,高浓度CO2对胞外碳酸酐酶活性具有明显的抑制作用,小新月菱形藻通过调整光系统II的能量流动和能量利用效率以适应高浓度CO2的环境.