丹参和钙通道拮抗剂抗LDL氧化作用的对比研究

提取健康成人血浆中的低密度脂蛋白（ＬＤＬ），与丹参和硝苯吡啶、尼索地平及Ｃｕ透析，分别于透析后２４、４８及７２小时从各组ＬＤＬ中提取脂类进行薄层层析（ＴＬＣ）。结果显示：①ｃｕ可使ＬＤＬ氧化成ｏｘＬＤＬ，ＴＬＣ示ｘ带、游离脂肪酸（ＦＦＡ）、磷脂（ＰＬ）增加，而胆固醇酯（ＣＥ）及甘油三酯（ＴＧ）减少；②钙通道拮抗剂＋Ｃｕ透析与单纯Ｃｕ透析结果相似；③丹参＋Ｃｕ透析的ｘ带显著低于Ｃｕ透析组及钙通道拮抗剂组，且脂质成分与透析前相似。结果表明，Ｘ带可作为脂质过氧化的一个重要指标，丹参具有拮抗ＬＤＬ氧化的作用，这可能是其防治心脑血管病的重要机理之一。

脑出血后智力障碍与局部脑血流量相关性研究

作者用长谷川智力测定量表法测定了６１例脑出血患者的智力，用ＳＰＥＣＴ定量测定法测定了双侧大脑半球各１５个脑区的局部血流量，并计算其比值。分析了脑出血、局部脑血流量、局部脑血流量比值与长谷川分数的关系。结果提示脑出血时，除由于病变直接破坏与智力有关的结构外，左半球（特别是左颞叶及左缘上回和角回）局部脑血流量下降，缘上回和角回、颞上回后部、颞中回后部、颞下回局部脑血流量比值失衡亦可能是造成智力下降的原因。

oxLDL对血管内皮细胞PECAM基因表达的影响

培养人脐带静脉内皮细胞（ＥＣ），提取人血浆低密度脂蛋白（ＬＤＬ）并制成氧化型低密度脂蛋白（ｏｘＬＤＬ），分别与血管ＥＣ培养后提取各组ＲＮＡ，进行电泳及Ｎｏｒｔｈｅｒｎ印迹杂交，用人β－ａｃｔｉｎ基因探针和人ＰＥＣＡＭ基因探针与各组ＲＮＡ进行杂交。结果显示：ＥＣ在Ｅ－ＧＭＵＶ培养液中生长度好并可多次传代培养；用ｏｘＬＤＬ处理ＥＣ２４ｈ可导致细胞死亡；用一步法提取单层培养ＥＣ的ＲＮＡ效果较好；用地高辛标记人β－ａｃｔｉｎ基因探针与各组ＲＮＡ杂交均可见杂交带，而未见ＰＥＣＡＭ基因表达。提示单层培养人血管ＦＣ适于基因表达的研究；ｏｘＬＤＬ对血管ＥＣ有细胞毒性，但不能促进ＰＥＣＡＭ基因表达。

卒中部位及局部脑血流量对智力的影响

用长谷川智力测定量表法测定了１８９例卒中患者的智力，分析了病变部位、脑萎缩及局部脑血流量（ｒＣＢＦ）对智力的影响．其中６９例患者长谷川分数低于２１．５分．当病变位于左半球时，属痴呆或可疑痴呆者达４２．９％；而病变位于右半球时，仅２３．１％属痴呆及可疑痴呆．ｒＣＢＦ与长谷川分数之间的相关分析显示：左额、右颞及左顶叶的ｒＣＢＦ与长谷川分数呈极显著正相关．提示左半球在智力活动中起主导作用．脑萎缩亦是影响智力的因素．

THE EFFECT OF RADIX SALVIAE MILTIORRHIZAE COMPOSITA AND LIGUSTRAZINE ON LIPID PEROXIDATION IN LOW DENSITY LIPOPROTEIN DUE TO COPPER DICHLORIDE

It is well known that lipid peroxide in low density lipoprotein (LDL) plays an important role in atherosclerosis and atherosclerotic diseases. We used oxidized LDL generated by incu-bating LDL from healthy persons with copper dichloride as a model to investigate the anti-lipid-peroxide property of Radix Salviae Miltiorrhizae Composita (RSMC) and ligustrazine. The changes in concentrations of lipid peroxide and lipids in LDL due to Cu++ were studied, and the effects of RSMC and ligustrazine on the changes were studied. The re-sults in our study indicate that RSMC has a potential role on and-lipid-peroxidation, but it was not found that ligustrazine has similar anti-peroxidation action.

THE EFFECT OF RADIX SALVIAE MILTIORRHIZAE COMPOSITA ON PEROXIDATION OF LOW DENSITY LIPOPROTEIN DUE TO COPPER DICHLORIDE

It is well known that plasma lipoprotein, particularly oxidized LDL, plays an important rolein the pathogenesis of atherosclerosis and atherosclerotic diseases. We used oxidized LDLgenerated by incubating LDL from healthy Persons with copper dichloride as a model to in-vestigate the antioxidate property of Radix Salviae Miltiorrhizae Composita (RSMC). Onphotos, the spot X1 and the spot X2 were clearly found in the control group after the dislysisinto copper dichloride for 24 and 48 hours, but they could not found in the RSMC group.The analysis of the constituents of lipids in LDL (by charring method) showed that afterdislysis the percentages of the spot X1 and the spot X2 in the RSMC group weresignificantly lower tban those in the control group (P【 0.01). The results suggest thatRSMC plays a potential role in antioxidation of lipuls or LDL.

兔脑缺血后局部脑血流量和皮质脑电图变化及与氧化型低密度脂蛋白的关系

目的研究高胆固醇和高糖饮食对脑缺血后局部脑血流量（ｒＣＢＦ）和皮质脑电图（ＥＥＧ）的影响及与氧化型低密度脂蛋白（ｏｘＬＤＬ）的关系。方法对４９只兔分别给予１％胆固醇、１０％糖水和标准食３种饮食共５周，行颈总动脉结扎或颈内动脉栓塞，同时同点连续记录皮质ＥＥＧ和每３０分钟测１次ｒＣＢＦ，并提取血浆低密度脂蛋白（ＬＤＬ）进行薄层层析（ＴＬＣ）和测定脂质过氧化物（ＬＰＯ）及血脂。结果（１）标准食兔较栓塞组ｒＣＢＦ及皮质ＥＥＧ总能量和平均频率显著降低（Ｐ值＜０．０１），糖水组与标准食组相似；（２）各组皮质ｒＣＢＦ与ＥＥＧ总能量呈显著正相关；（３）胆固醇食兔栓塞组和结扎组缺血侧ｒＣＢＦ及ＥＥＧ均显著降低，血浆和ＬＤＬ中ＬＰＯ含量显著增高，ＬＤＬ的ＴＬＣ分析示有较多Ｘ带形成。结论长期高胆固醇食导致血浆和ＬＤＬ中ＬＰＯ及Ｘ带增多，ｏｘＬＤＬ形成，加速动脉粥样硬化，加重脑缺血时ｒＣＢＦ和ＥＥＧ能量和频率的降低。

巴曲酶促进小鼠腹腔巨噬细胞吞噬过氧化低密度脂蛋白

采用小鼠腹腔巨噬细胞在含有过氧化低密度脂蛋白（Ｐｏｘ－ＬＤＬ）的培养液中培养，并用巴曲酶（ｂａｔｒｏｘｏｂｉｎ）进行干预，运用透射电子显微镜进行观察，旨在了解巴曲酶对巨噬细胞吞噬ｐｏｘ－ＬＤＬ的影响。研究结果表明；小鼠腹腔巨噬细胞与纯培养剂ＤＭＥＭ正常低密度脂蛋白（ｎ－ＬＤＬ），以及ｎ－ＬＤＬ加巴曲酶培养４小时后，细胞的超微结构与培养前相似，而与Ｐｏｘ－ＬＤＬ一起培养４小时后细胞胞浆内则含大量的脂滴，加入巴曲酶及小鼠腹腔巨噬细胞内脂滴的生成明显加快。