This work was supported by a grant from National Education Committee of China.Abbreviations: GST, glutathione Stransferase; GSTπ, GST pi class; DEN, diethylnitrosamine; MNU, Nmethylnitrosourea; CSC, cigarette smoke c...This work was supported by a grant from National Education Committee of China.Abbreviations: GST, glutathione Stransferase; GSTπ, GST pi class; DEN, diethylnitrosamine; MNU, Nmethylnitrosourea; CSC, cigarette smoke condensate; HFL, human fetal lung; CDNB, 1chloro2,4dinitrobenzene. Objective : To investigate the relationship between the GSTs, GSTπ expression and initiation of lung carcinogenesis. Methods: The Rat1 cells were transformed by carcinogens (DEN, MNU and CSC) treated fetal lung DNA for 24 h. Results: The GSTs activities toward 1chloro2, 4dinitrobenzene (CDNB) in transformed cells were significantly higher than in the solvent control cells (P<005). GSTπ content and GSTπ mRNA expression level of transformed cells were also higher than those of control cells which were performed by ELISA and Northern blotting method respectively. The results indicated that the higher GSTS activities of transformed cells were due to the increase of GSTπ content and the GSTπ mRNA overexpressing may be responsible for the increase of GSTπ protein level of the transformed cells. Conclusion: The changes of GSTs and GSTπ may be considered as the one of the biomarkers of the initiation of human lung carcinogenesis.展开更多
文摘This work was supported by a grant from National Education Committee of China.Abbreviations: GST, glutathione Stransferase; GSTπ, GST pi class; DEN, diethylnitrosamine; MNU, Nmethylnitrosourea; CSC, cigarette smoke condensate; HFL, human fetal lung; CDNB, 1chloro2,4dinitrobenzene. Objective : To investigate the relationship between the GSTs, GSTπ expression and initiation of lung carcinogenesis. Methods: The Rat1 cells were transformed by carcinogens (DEN, MNU and CSC) treated fetal lung DNA for 24 h. Results: The GSTs activities toward 1chloro2, 4dinitrobenzene (CDNB) in transformed cells were significantly higher than in the solvent control cells (P<005). GSTπ content and GSTπ mRNA expression level of transformed cells were also higher than those of control cells which were performed by ELISA and Northern blotting method respectively. The results indicated that the higher GSTS activities of transformed cells were due to the increase of GSTπ content and the GSTπ mRNA overexpressing may be responsible for the increase of GSTπ protein level of the transformed cells. Conclusion: The changes of GSTs and GSTπ may be considered as the one of the biomarkers of the initiation of human lung carcinogenesis.
