Objective: To evaluate the antitumor effect of BRM, a Chinese traditional medical compound herb, and its mechanism. Methods: Tumor inhibition was tested in vivo on mice with subcutaneous transplanted tumor cells in vi...Objective: To evaluate the antitumor effect of BRM, a Chinese traditional medical compound herb, and its mechanism. Methods: Tumor inhibition was tested in vivo on mice with subcutaneous transplanted tumor cells in vitro with and MTT method and tumor cell apoptosis test including cell electric morphology, agarose gel electrophoresis and flow cytometry. Results: It was found that BRM could the resistance to the attack of 5×106 of mouse pancreascarcinoma (MPC-83), Ehrlich ascite tumor (EAC), mouse sarcoma (S-180), and mouse glioblastoma (G422) cells, with the inhibition rate of tumor weight of 66.2%, 50.9%, 47.3%, and 38.7% respectively. The inhibition rate of Suzhou human glioma (SHG-44), human breast carcinoma (MCF-7), and human pancreas carcinoma (PANC1) in vitro were 92.2% (BRM 1:10), 85.1% (BRM 1:10), 48.3% (BRM 1:10), respectively. Meanwhile, BRM was able to induce apoptosis in several human tumor cell lines such as SHG-44 and MCF-7 cells. Morphological changes including cell shrinkage and condensation of chromosomes were observed with electric microscope. Agarose gel electrophoresis of DNA from SHG-44 and MCF-7 cells treated with BRM extracts (1:80 to 1:20) for 24 h, 40 h revealed typical DNA “Ladder” pattern. The early stage and middle-late stage apoptosis increased in SHG-44 and MCF-7 cells treated with BRM extracts (BRM 1:160 to 1:40) from 14 h to 48 h by Annexin-V/PI flow cytometry one analysis. Conclusion: BRM has obvious antitumor bioactivity in vivo to MPC-83, EAC, S-180, and G422 mouse cell strains, and in vitro to SHG-44, MCF-7 human cell lines. Water extracts of BRM could induce apoptosis.展开更多
Objective: To investigate the antitumor activity and the mechanism of BRM-SJS on breast cancer cells. Methods: Flow cytometry, DNA agarose gel electrophoresis and other techniques were used to study the in vitro and...Objective: To investigate the antitumor activity and the mechanism of BRM-SJS on breast cancer cells. Methods: Flow cytometry, DNA agarose gel electrophoresis and other techniques were used to study the in vitro and in vivo inhibitory effect on BcaP-37 cells by BRM-SJS. Results: BRM-SJS showed an inhibitory rate of 33.8% on in vivo transplanted tumor (P<0.05, compared with control). The flow cytometry analysis of BRM-SJS treated BcaP-37 (2.5 靘ol/L, 5 mol/L, 10 靘ol/L for 48 h and 72 h) revealed typical sub-G1 peak. The specific DNA Ladders were exhibited with BRM-SJS BcaP-37 cells treated. Conclusion: BRM-SJS has marked antitumor activity on BcaP-37 and its inhibitory effects on tumor were realized by both induction of apoptosis and necrosis of the tumor cells.展开更多
文摘Objective: To evaluate the antitumor effect of BRM, a Chinese traditional medical compound herb, and its mechanism. Methods: Tumor inhibition was tested in vivo on mice with subcutaneous transplanted tumor cells in vitro with and MTT method and tumor cell apoptosis test including cell electric morphology, agarose gel electrophoresis and flow cytometry. Results: It was found that BRM could the resistance to the attack of 5×106 of mouse pancreascarcinoma (MPC-83), Ehrlich ascite tumor (EAC), mouse sarcoma (S-180), and mouse glioblastoma (G422) cells, with the inhibition rate of tumor weight of 66.2%, 50.9%, 47.3%, and 38.7% respectively. The inhibition rate of Suzhou human glioma (SHG-44), human breast carcinoma (MCF-7), and human pancreas carcinoma (PANC1) in vitro were 92.2% (BRM 1:10), 85.1% (BRM 1:10), 48.3% (BRM 1:10), respectively. Meanwhile, BRM was able to induce apoptosis in several human tumor cell lines such as SHG-44 and MCF-7 cells. Morphological changes including cell shrinkage and condensation of chromosomes were observed with electric microscope. Agarose gel electrophoresis of DNA from SHG-44 and MCF-7 cells treated with BRM extracts (1:80 to 1:20) for 24 h, 40 h revealed typical DNA “Ladder” pattern. The early stage and middle-late stage apoptosis increased in SHG-44 and MCF-7 cells treated with BRM extracts (BRM 1:160 to 1:40) from 14 h to 48 h by Annexin-V/PI flow cytometry one analysis. Conclusion: BRM has obvious antitumor bioactivity in vivo to MPC-83, EAC, S-180, and G422 mouse cell strains, and in vitro to SHG-44, MCF-7 human cell lines. Water extracts of BRM could induce apoptosis.
文摘Objective: To investigate the antitumor activity and the mechanism of BRM-SJS on breast cancer cells. Methods: Flow cytometry, DNA agarose gel electrophoresis and other techniques were used to study the in vitro and in vivo inhibitory effect on BcaP-37 cells by BRM-SJS. Results: BRM-SJS showed an inhibitory rate of 33.8% on in vivo transplanted tumor (P<0.05, compared with control). The flow cytometry analysis of BRM-SJS treated BcaP-37 (2.5 靘ol/L, 5 mol/L, 10 靘ol/L for 48 h and 72 h) revealed typical sub-G1 peak. The specific DNA Ladders were exhibited with BRM-SJS BcaP-37 cells treated. Conclusion: BRM-SJS has marked antitumor activity on BcaP-37 and its inhibitory effects on tumor were realized by both induction of apoptosis and necrosis of the tumor cells.
