目的观察出生后至成年小鼠小肠Cajal间质细胞(interstitial cells of Cajal,ICC)、氮能神经元、胆碱能神经元的发育情况。方法取出生后7d、28d和60d小鼠小肠制作全层铺片,利用(免疫)组织化学等技术分别显示ICC、氮能神经元、胆碱能神经...目的观察出生后至成年小鼠小肠Cajal间质细胞(interstitial cells of Cajal,ICC)、氮能神经元、胆碱能神经元的发育情况。方法取出生后7d、28d和60d小鼠小肠制作全层铺片,利用(免疫)组织化学等技术分别显示ICC、氮能神经元、胆碱能神经元的数量和形态。结果小鼠生后小肠ICC的数量明显增多,但单位面积细胞数量随小肠长度和面积的增加而降低,在生后28d达到成体水平。而氮能神经元和胆碱能神经元的数量生后即达成体水平,随年龄增加仅见神经突起增长、神经纤维增粗以及神经网络的密度变疏等改变。结论小鼠小肠ICC和胃肠壁内的肠神经系统(enteric nervous system,ENS)神经元的发育并非同步,ICC的发育成熟明显晚于ENS,提示出生后早期局部微环境更易影响ICC的发育与成熟,可能与部分婴幼儿胃肠运动功能障碍疾病的发生发展有关。展开更多
Summary： Relationship between ATP changes of rabbit blood and postmortem interval （PMI） was studied. Twenty-four healthy rabbits were sacrificed and randomly divided into 3 groups with 8 rab- bits of each group. Th...Summary： Relationship between ATP changes of rabbit blood and postmortem interval （PMI） was studied. Twenty-four healthy rabbits were sacrificed and randomly divided into 3 groups with 8 rab- bits of each group. The bodies of three groups were placed in calorstat at temperature of 15℃, 25℃ and 35℃, respectively. The blood from the right ventricle was sampled through indwelling needle each 4 h until 72 h after death. ATP levels in the blood samples were measured by using ATP fluo- rescence rapid detection technique at different PMIs. Blood ATP levels slightly increased in the early stage after death and then constantly declined at all temperatures （15℃, 25℃, and 35℃）. Cubic polynomial regression equations with log[ATP] as dependent variable （y） and PMI as independent variable （x） at different temperatures and the optimal time period were established as followed： Under 15℃ and during 16-64 h after death, y=-3.027×10^-5x^3＋0.003x^2-0.096x-10.625 （Ra^2=0.992, P〈0.001）; under 25℃ and during 8-56 h after death, y=-2.921×10^-5x^3＋0.002x^2- 0.059x-11.186 （Ra^2=0.989, P〈0.001）; under 35℃ and during 4-36 h after death, y=-9.769×10^-5x^3＋ 0.005x^2 -0.117x-11.166 （Ra^2=0.991, P〈0.001）. The changes in ATP levels in blood collected from right ven- tricle of rabbit cadavers showed relatively stable and regular degradation within 72 h after death at different temperatures.展开更多
基金supported by the National Natural Science Foundation of China (No. 81072505)
文摘Summary： Relationship between ATP changes of rabbit blood and postmortem interval （PMI） was studied. Twenty-four healthy rabbits were sacrificed and randomly divided into 3 groups with 8 rab- bits of each group. The bodies of three groups were placed in calorstat at temperature of 15℃, 25℃ and 35℃, respectively. The blood from the right ventricle was sampled through indwelling needle each 4 h until 72 h after death. ATP levels in the blood samples were measured by using ATP fluo- rescence rapid detection technique at different PMIs. Blood ATP levels slightly increased in the early stage after death and then constantly declined at all temperatures （15℃, 25℃, and 35℃）. Cubic polynomial regression equations with log[ATP] as dependent variable （y） and PMI as independent variable （x） at different temperatures and the optimal time period were established as followed： Under 15℃ and during 16-64 h after death, y=-3.027×10^-5x^3＋0.003x^2-0.096x-10.625 （Ra^2=0.992, P〈0.001）; under 25℃ and during 8-56 h after death, y=-2.921×10^-5x^3＋0.002x^2- 0.059x-11.186 （Ra^2=0.989, P〈0.001）; under 35℃ and during 4-36 h after death, y=-9.769×10^-5x^3＋ 0.005x^2 -0.117x-11.166 （Ra^2=0.991, P〈0.001）. The changes in ATP levels in blood collected from right ven- tricle of rabbit cadavers showed relatively stable and regular degradation within 72 h after death at different temperatures.
