To identify whether Puerarin (Puer) has blocking effects on L type calcium channel Methods We used whole cell recording of patch clamp techniques to analyse calcium channel current in ventricular myocytes isolate...To identify whether Puerarin (Puer) has blocking effects on L type calcium channel Methods We used whole cell recording of patch clamp techniques to analyse calcium channel current in ventricular myocytes isolated from Langendorff guinea pig hearts by collagenase Results In control group, peak calcium channel current was decreased by 10 6% and 29 8% at 5, 10?min of recording time, respectively For the same period, 0 1?mmol/L Puer reduced calcium current by 23 9% and 72 4% 0 1 and 1?mmol/L Puer inhibited the amplitude of peak Calcium channel current by 40 6% and 63 2% after perfusing for 10?min Compared with control group in which “rundown' phenomenon was observed, Puer also demonstrated its blocking effect on L type channel While washout of Puer with perfusing solution, calcium channel current went down further instead of recovery Current voltage (Ⅰ Ⅴ) curves showed that the levels of calcium channel current were obviously decreased by Puer (both 0 1?mmo/L and 1?mmo/L) form -40?mV to+10?mV Conclusion Puer has blocking effect on L type calcium channel in a concentration dependent manner展开更多
文摘To identify whether Puerarin (Puer) has blocking effects on L type calcium channel Methods We used whole cell recording of patch clamp techniques to analyse calcium channel current in ventricular myocytes isolated from Langendorff guinea pig hearts by collagenase Results In control group, peak calcium channel current was decreased by 10 6% and 29 8% at 5, 10?min of recording time, respectively For the same period, 0 1?mmol/L Puer reduced calcium current by 23 9% and 72 4% 0 1 and 1?mmol/L Puer inhibited the amplitude of peak Calcium channel current by 40 6% and 63 2% after perfusing for 10?min Compared with control group in which “rundown' phenomenon was observed, Puer also demonstrated its blocking effect on L type channel While washout of Puer with perfusing solution, calcium channel current went down further instead of recovery Current voltage (Ⅰ Ⅴ) curves showed that the levels of calcium channel current were obviously decreased by Puer (both 0 1?mmo/L and 1?mmo/L) form -40?mV to+10?mV Conclusion Puer has blocking effect on L type calcium channel in a concentration dependent manner
