潍坊市益都中心医院通过建立综合考评体系,导入以资源为基础的相对价值比率(Resource Based Relative Value Scale,RBRVS)方法考核工作量,设计专项绩效以加强关键流程和关键技术考核力度,结合综合目标管理强化质量管理和内涵建设。综合...潍坊市益都中心医院通过建立综合考评体系,导入以资源为基础的相对价值比率(Resource Based Relative Value Scale,RBRVS)方法考核工作量,设计专项绩效以加强关键流程和关键技术考核力度,结合综合目标管理强化质量管理和内涵建设。综合考评体系运行后国家三级医院关键绩效考核指标明显改善,员工积极性提高,精细化管理能力同步得到提升,为公立医院绩效改革实践提供了借鉴。展开更多
We previously functionally characterized a novel marine microbial GDSL lipase MT6 and identified that the stereo-selectivity of MT6 was opposite to that of other common lipases in trans-esterification reactions.Herein...We previously functionally characterized a novel marine microbial GDSL lipase MT6 and identified that the stereo-selectivity of MT6 was opposite to that of other common lipases in trans-esterification reactions.Herein,we have investigated the use of MT6 in stereo-selective biocatalysis through direct hydrolysis reactions.Notably,the stereo-selectivity of MT6 was also demonstrated to be opposite to that of other common lipases in hydrolysis reactions.Parameters,including temperature,organic co-solvents,pH,ionic strength,catalyst loading,substrate concentration,and reaction time,affecting the enzymatic resolution of racemic 1-phenylethyl acetate were further investigated,with the e.e.of the final（S）-l-Phenylethanol product and the conversion being 97%and 28.5%,respectively,after process optimization.The lengths of side chains of 1-phenylethyl esters greatly affected the stereo-selectivity and conversion during kinetic resolutions.MT6 is a novel marine microbial GDSL lipase exhibiting opposite stereo-selectivities than other common lipases in both trans-esterification reactions and hydrolysis reactions.展开更多
文摘潍坊市益都中心医院通过建立综合考评体系,导入以资源为基础的相对价值比率(Resource Based Relative Value Scale,RBRVS)方法考核工作量,设计专项绩效以加强关键流程和关键技术考核力度,结合综合目标管理强化质量管理和内涵建设。综合考评体系运行后国家三级医院关键绩效考核指标明显改善,员工积极性提高,精细化管理能力同步得到提升,为公立医院绩效改革实践提供了借鉴。
基金supported by the Strategic Priority Research Program of Chinese Academy of Sciences(XDA11030404),the Key Project from Chinese Academy of Sciences(KGZD-EW-606)the National Natural Science Foundation of China(21302199)Guangzhou Science and Technology Plan Projects(201510010012)
文摘We previously functionally characterized a novel marine microbial GDSL lipase MT6 and identified that the stereo-selectivity of MT6 was opposite to that of other common lipases in trans-esterification reactions.Herein,we have investigated the use of MT6 in stereo-selective biocatalysis through direct hydrolysis reactions.Notably,the stereo-selectivity of MT6 was also demonstrated to be opposite to that of other common lipases in hydrolysis reactions.Parameters,including temperature,organic co-solvents,pH,ionic strength,catalyst loading,substrate concentration,and reaction time,affecting the enzymatic resolution of racemic 1-phenylethyl acetate were further investigated,with the e.e.of the final（S）-l-Phenylethanol product and the conversion being 97%and 28.5%,respectively,after process optimization.The lengths of side chains of 1-phenylethyl esters greatly affected the stereo-selectivity and conversion during kinetic resolutions.MT6 is a novel marine microbial GDSL lipase exhibiting opposite stereo-selectivities than other common lipases in both trans-esterification reactions and hydrolysis reactions.
