In order to identify an unknown whale vertebra’s species,genomic DNA is extracted from the bone powder by proteinase K and phenol/chloroform,and a partial Cyt b gene sequence as long as 565 bp is amplified by PCR(Pol...In order to identify an unknown whale vertebra’s species,genomic DNA is extracted from the bone powder by proteinase K and phenol/chloroform,and a partial Cyt b gene sequence as long as 565 bp is amplified by PCR(Polymerase Chain Reaction).The blasting result in GenBank shows that the vertebra sample has maximal similarity with Balaenoptera omurai,it is 99%.It indicates that the vertebra is Balaenoptera omurai.The phylogenetic tree based on UPGMA is consistent with the blasting result.Analysis of software MEGA shows that the Cyt b gene sequence of the vertebra sample has 1-2 nucleotides which are different from the homologous sequence of B.omurai(accession AB201257,AB201256),thus,the sequence is a new haplotype.This is the first record of B.omurai which distributes in the South China Sea.展开更多
文摘In order to identify an unknown whale vertebra’s species,genomic DNA is extracted from the bone powder by proteinase K and phenol/chloroform,and a partial Cyt b gene sequence as long as 565 bp is amplified by PCR(Polymerase Chain Reaction).The blasting result in GenBank shows that the vertebra sample has maximal similarity with Balaenoptera omurai,it is 99%.It indicates that the vertebra is Balaenoptera omurai.The phylogenetic tree based on UPGMA is consistent with the blasting result.Analysis of software MEGA shows that the Cyt b gene sequence of the vertebra sample has 1-2 nucleotides which are different from the homologous sequence of B.omurai(accession AB201257,AB201256),thus,the sequence is a new haplotype.This is the first record of B.omurai which distributes in the South China Sea.
