Baicalin facilitates the effectiveness of both penicillin and Notopterygium ethanol extracts (NEE) on Staphylococcus aureus. Two Staphylococcus aureus strains, ATCC29213 and 03-21 (a clinical isolate), were tested. Wh...Baicalin facilitates the effectiveness of both penicillin and Notopterygium ethanol extracts (NEE) on Staphylococcus aureus. Two Staphylococcus aureus strains, ATCC29213 and 03-21 (a clinical isolate), were tested. When combined with 128 μg·mL?1 of baicalin, the minimum inhibitory concentration (MIC) of penicillin was reduced from 0.25 μg·mL?1 to 0.06 μg·mL?1, while that of NEE was reduced from 32 μg·mL?1 to 8 μg·mL?1 against ATCC29213. Against the 03-21 strain, the MIC of penicillin was reduced from 4 μg·mL?1 to 1 μg·mL?1, while that of NEE was reduced from 32 μg·mL?1 to 4 μg·mL?1. Viable number counting results show that combination of baicalin and penicillin acts on the killing mechanism, while the baicalin and NEE acts on the inhibition. These results show that baicalin acts as a synergistic ally with both penicillin and NEE but has different modes of action.展开更多
The objective of this study is to quantify the puerarin in rat plasma following oral administration of TZ18 and compare the pharmacokinetics characteristics of puerarin in normal rats with that in depression model rat...The objective of this study is to quantify the puerarin in rat plasma following oral administration of TZ18 and compare the pharmacokinetics characteristics of puerarin in normal rats with that in depression model rats. A high performance liquid chromatography method was used to quantify the puerarin due to its good selectivity and linearity (coefficient correlation, r2 = 0.9991) within the tested range (0.028-0.889 μg·mL-1). Intra- and inter-day precision coefficients of variation and accuracy bias were acceptable (Maximum coeffi- cient of variation was 5.74% for intra-day and 3.09% for inter-day) over the entire range. The recoveries were found to be 98.3%, 101.4%, and 103.4% for concentrations of 0.028, 0.222, and 0.444 μg·mL-1, re- spectively. The concentration-time curves for both normal rats and depression model rats were fit to a two- compartment model with the first order absorption. The results show significant differences in the main phar- macokinetic parameters of peak time, peak concentration, and the area under the concentration-time curve between the two kinds of rats.展开更多
基金National Natural Science Foundation of China (Grant No.30973896,30801523 and 81073092)the National S&T Major Special Project for New Drug R&D of China(Grant No. 2009ZX09103 -301,2009ZX09502 and 2011ZX09101-002-11 )
基金Supported by the Basic Research Foundation of Tsinghua University
文摘Baicalin facilitates the effectiveness of both penicillin and Notopterygium ethanol extracts (NEE) on Staphylococcus aureus. Two Staphylococcus aureus strains, ATCC29213 and 03-21 (a clinical isolate), were tested. When combined with 128 μg·mL?1 of baicalin, the minimum inhibitory concentration (MIC) of penicillin was reduced from 0.25 μg·mL?1 to 0.06 μg·mL?1, while that of NEE was reduced from 32 μg·mL?1 to 8 μg·mL?1 against ATCC29213. Against the 03-21 strain, the MIC of penicillin was reduced from 4 μg·mL?1 to 1 μg·mL?1, while that of NEE was reduced from 32 μg·mL?1 to 4 μg·mL?1. Viable number counting results show that combination of baicalin and penicillin acts on the killing mechanism, while the baicalin and NEE acts on the inhibition. These results show that baicalin acts as a synergistic ally with both penicillin and NEE but has different modes of action.
文摘The objective of this study is to quantify the puerarin in rat plasma following oral administration of TZ18 and compare the pharmacokinetics characteristics of puerarin in normal rats with that in depression model rats. A high performance liquid chromatography method was used to quantify the puerarin due to its good selectivity and linearity (coefficient correlation, r2 = 0.9991) within the tested range (0.028-0.889 μg·mL-1). Intra- and inter-day precision coefficients of variation and accuracy bias were acceptable (Maximum coeffi- cient of variation was 5.74% for intra-day and 3.09% for inter-day) over the entire range. The recoveries were found to be 98.3%, 101.4%, and 103.4% for concentrations of 0.028, 0.222, and 0.444 μg·mL-1, re- spectively. The concentration-time curves for both normal rats and depression model rats were fit to a two- compartment model with the first order absorption. The results show significant differences in the main phar- macokinetic parameters of peak time, peak concentration, and the area under the concentration-time curve between the two kinds of rats.
