For a long time phenolic additions have been widely used for the preservation of insulin preparation, but its action mechanism has not been elucidated. In 1958 Schlichkrull first obtained monoclinic insulin crystals i...For a long time phenolic additions have been widely used for the preservation of insulin preparation, but its action mechanism has not been elucidated. In 1958 Schlichkrull first obtained monoclinic insulin crystals in a system with 1.0% phenol which differs remarkably from those crystal growing systems of rhombohedral 2Zn and 4Zn insulin. Kreyenbuhl and Rosenberg also reported in 1964 that the presence of either phenol or m-cresol is an absolute requirement for the formation of Zn展开更多
Based on the crystal growth system of rhombohedral 2Zn-insulin,the phase transition ofinsulin crystals has been investigated with the phenol concentration as an independent component.The dia-gram of the phase relation...Based on the crystal growth system of rhombohedral 2Zn-insulin,the phase transition ofinsulin crystals has been investigated with the phenol concentration as an independent component.The dia-gram of the phase relationship in this crystal growth system was established,and two points of phase transi-tion were found.The transition point Ⅰ indicates the phase transition between rhombohedral 2Zn-insulin crys-tal and rhombohedral 4Zn-insulin crystal,and these two phases coexist within a narrow region of phenol con-centration (0.028%-0.029% (g/ml)).Point Ⅱ at 0.76%-0.77% (g/ml) of phenol concentration showsthe phase transition between rhombohcdral crystal and monoclinic crystals,and a new phase of monocliniccrystal (B-form monoclinic insulin crystal) has been observed.This paper reports the diagram of phase rela-tionship obtained from our experiments,and analyses and discusses the dependence of phase transition of in-sulin crystals on phenol concentration in crystal growth system.展开更多
基金Project supported by Academia Sinica and the National Natural Science Foundation of China
文摘For a long time phenolic additions have been widely used for the preservation of insulin preparation, but its action mechanism has not been elucidated. In 1958 Schlichkrull first obtained monoclinic insulin crystals in a system with 1.0% phenol which differs remarkably from those crystal growing systems of rhombohedral 2Zn and 4Zn insulin. Kreyenbuhl and Rosenberg also reported in 1964 that the presence of either phenol or m-cresol is an absolute requirement for the formation of Zn
基金the Foundation of Chinese Academy of Sciencesthe National Natural Science Foundation of China
文摘Based on the crystal growth system of rhombohedral 2Zn-insulin,the phase transition ofinsulin crystals has been investigated with the phenol concentration as an independent component.The dia-gram of the phase relationship in this crystal growth system was established,and two points of phase transi-tion were found.The transition point Ⅰ indicates the phase transition between rhombohedral 2Zn-insulin crys-tal and rhombohedral 4Zn-insulin crystal,and these two phases coexist within a narrow region of phenol con-centration (0.028%-0.029% (g/ml)).Point Ⅱ at 0.76%-0.77% (g/ml) of phenol concentration showsthe phase transition between rhombohcdral crystal and monoclinic crystals,and a new phase of monocliniccrystal (B-form monoclinic insulin crystal) has been observed.This paper reports the diagram of phase rela-tionship obtained from our experiments,and analyses and discusses the dependence of phase transition of in-sulin crystals on phenol concentration in crystal growth system.
