The recombinant GBV-C/HGV NS3 protein was expressed in Sf9 insect cells and analyzed by SDS-PAGE. A protein band about Mr 43810 was demonstrated on polyacrylamide gel electrophoresis and this protein amounted to more ...The recombinant GBV-C/HGV NS3 protein was expressed in Sf9 insect cells and analyzed by SDS-PAGE. A protein band about Mr 43810 was demonstrated on polyacrylamide gel electrophoresis and this protein amounted to more than 30% of the total cell proteins. Recombinant NS3 protein was purified by Ni-NTA column. Western blot showed that this recombinant protein could react with GBV-C/HGV RNA positive mixed sera. The recombinant GBV-C/HGV NS3 protein could be used to detect GBV-C/HGV infection and will supply material for study of structure and function of this protein.展开更多
Forty-two patients with hepatocellular carcinoma (HCC) were examined for hepatitis C virus (HCV) RNA in liver tissues by reverse transcription-polymerase chain reaction (RT-PCR) with primers deduced from 5'-non-co...Forty-two patients with hepatocellular carcinoma (HCC) were examined for hepatitis C virus (HCV) RNA in liver tissues by reverse transcription-polymerase chain reaction (RT-PCR) with primers deduced from 5'-non-coding region (5'-NCR). HCV liver samples of展开更多
Three synthetic peptides representing distinct antigenic epitopes from three genomic regions of hepatitis E virus (HEV) were used to develop enzyme-linked immunoserbent assay ELISA for detecting antibodies to HEV. Bot...Three synthetic peptides representing distinct antigenic epitopes from three genomic regions of hepatitis E virus (HEV) were used to develop enzyme-linked immunoserbent assay ELISA for detecting antibodies to HEV. Both IgG and IgM antibodies to HEV were d展开更多
文摘The recombinant GBV-C/HGV NS3 protein was expressed in Sf9 insect cells and analyzed by SDS-PAGE. A protein band about Mr 43810 was demonstrated on polyacrylamide gel electrophoresis and this protein amounted to more than 30% of the total cell proteins. Recombinant NS3 protein was purified by Ni-NTA column. Western blot showed that this recombinant protein could react with GBV-C/HGV RNA positive mixed sera. The recombinant GBV-C/HGV NS3 protein could be used to detect GBV-C/HGV infection and will supply material for study of structure and function of this protein.
文摘Forty-two patients with hepatocellular carcinoma (HCC) were examined for hepatitis C virus (HCV) RNA in liver tissues by reverse transcription-polymerase chain reaction (RT-PCR) with primers deduced from 5'-non-coding region (5'-NCR). HCV liver samples of
文摘Three synthetic peptides representing distinct antigenic epitopes from three genomic regions of hepatitis E virus (HEV) were used to develop enzyme-linked immunoserbent assay ELISA for detecting antibodies to HEV. Both IgG and IgM antibodies to HEV were d