目的通过RNA干扰技术阻断人骨关节炎呈现OA滑膜细胞WISP-1信号通路,探索其在骨关节炎治疗中的应用前景。方法根据干扰RNA设计原则,合成并构建WISP-1 sh RNA质粒表达载体。将质粒表达载体转染入滑膜细胞并分组。转染24 h后测定细胞β-cat...目的通过RNA干扰技术阻断人骨关节炎呈现OA滑膜细胞WISP-1信号通路,探索其在骨关节炎治疗中的应用前景。方法根据干扰RNA设计原则,合成并构建WISP-1 sh RNA质粒表达载体。将质粒表达载体转染入滑膜细胞并分组。转染24 h后测定细胞β-catenin、WISP-1、MMP-3 m RNA相对表达水平。结果 OA滑膜细胞经过RNAi后WISP-1、MMP-3表达明显减少,意味着干扰WISP-1使得Wnt/β-catenin通路对MMP-3的诱导作用降低,也进一步证实了Wnt/β-atenin/WISP-1通路的存在。因此,WISP-1可以作为治疗骨关节炎一个理想的靶点。结论本实验中我们观察到,OA滑膜细胞经过RNAi后MMP-3表达明显减少,意味着Wnt/β-连环蛋白信号通路对MMP-3的诱导作用降低,进一步证实了Wnt/β-连环蛋白(catenin)/WISP-1通路的存在。展开更多
Objective： To explore the treatment methods and outcome of posterior wall fractures of the acetabulum. Methods： The data of 31 patients （25 males and 6 females, aged 19-59 years, mean： 40.5 years） with posterior ...Objective： To explore the treatment methods and outcome of posterior wall fractures of the acetabulum. Methods： The data of 31 patients （25 males and 6 females, aged 19-59 years, mean： 40.5 years） with posterior wall fractures of the acetabulum hospitalized in our department from 2002 to 2006 were analyzed retrospectively in this study. The types of fractures, number of fragments, combined dislocations, and sciatic nerve function were documented before admission. All the fractures were treated with open reduction and internal fixation. Based on the fracture type and site, either screws alone or reconstructive plates were used. The patients were immobilized for an average of 12 weeks before partial weight bearing was permitted. After follow-up for 12-70 months （43.6 months on average）, modified Merle d＇Aubigne score was adopted to evaluate the outcomes of the operations. Results： The percentages of the excellent, good, fair and poor results were 48.4%, 41.9%, 6.5%, and 3.3%, respectively, with a good to excellent rate of 90.2%. Idiopathic sciatic nerve injury occurred in only one case. Conclusions： The sciatic nerve should be routinely exposed and protected during the surgery. The type of fixation should be based on the fracture type and site. Prolonged immobilization may be helpful in improving the final outcomes.展开更多
Objective：To study the surgical treatment for distal humeral fractures in adults according to the follow-up results.Methods： Twenty-one cases （16 males and 5 females）of distal humeral fracture were included in thi...Objective：To study the surgical treatment for distal humeral fractures in adults according to the follow-up results.Methods： Twenty-one cases （16 males and 5 females）of distal humeral fracture were included in this study. The average age was 42.5 years （range： 37-52 years）. Fractures were classified according to the AO classification system.Nine cases of C1,8 C2 and 4 C3-type fractures were identified.Open reduction and internal fixation were performed in all cases. A tricep-reflecting approach was adopted, and either the AO orthogonal plating or parallel plating technique was chosen,based on the fracture type. The plaster cast was removed 3 weeks after operation. Rehabilitation was encouraged during this period and afterwards. The average follow-up time was 12.2 months （range： 8-28 months）. The outcome was scored according to Aitken and Rorabeek system.Results：No nerve injury,nonunion or failure of fixation was encountered during the operation and follow-up.However, ossifying myosifis occurred in one case.Conclusions：A triceps-reflecting approach can provide adequate exposure to the joint. The use of AO orthogohal plating or parallel plating techniques based on the type of fractures can provide rigid fixation for the fracture.展开更多
Posterolateral rotatory instability of the elbow describes a condition that radial head subluxation or dislocation occurs when forearm rotates externally in relation to humerus. It is difficult to diagnose and treat. ...Posterolateral rotatory instability of the elbow describes a condition that radial head subluxation or dislocation occurs when forearm rotates externally in relation to humerus. It is difficult to diagnose and treat. We reported a typical case which was confirmed by physical examination and MR images. Ligamentous insufficiency was confirmed under direct vision, and was reconstructed with triceps fascia as described by Gong et al with slight modification. Regain of full function was achieved one year after surgery.展开更多
文摘目的通过RNA干扰技术阻断人骨关节炎呈现OA滑膜细胞WISP-1信号通路,探索其在骨关节炎治疗中的应用前景。方法根据干扰RNA设计原则,合成并构建WISP-1 sh RNA质粒表达载体。将质粒表达载体转染入滑膜细胞并分组。转染24 h后测定细胞β-catenin、WISP-1、MMP-3 m RNA相对表达水平。结果 OA滑膜细胞经过RNAi后WISP-1、MMP-3表达明显减少,意味着干扰WISP-1使得Wnt/β-catenin通路对MMP-3的诱导作用降低,也进一步证实了Wnt/β-atenin/WISP-1通路的存在。因此,WISP-1可以作为治疗骨关节炎一个理想的靶点。结论本实验中我们观察到,OA滑膜细胞经过RNAi后MMP-3表达明显减少,意味着Wnt/β-连环蛋白信号通路对MMP-3的诱导作用降低,进一步证实了Wnt/β-连环蛋白(catenin)/WISP-1通路的存在。
文摘Objective： To explore the treatment methods and outcome of posterior wall fractures of the acetabulum. Methods： The data of 31 patients （25 males and 6 females, aged 19-59 years, mean： 40.5 years） with posterior wall fractures of the acetabulum hospitalized in our department from 2002 to 2006 were analyzed retrospectively in this study. The types of fractures, number of fragments, combined dislocations, and sciatic nerve function were documented before admission. All the fractures were treated with open reduction and internal fixation. Based on the fracture type and site, either screws alone or reconstructive plates were used. The patients were immobilized for an average of 12 weeks before partial weight bearing was permitted. After follow-up for 12-70 months （43.6 months on average）, modified Merle d＇Aubigne score was adopted to evaluate the outcomes of the operations. Results： The percentages of the excellent, good, fair and poor results were 48.4%, 41.9%, 6.5%, and 3.3%, respectively, with a good to excellent rate of 90.2%. Idiopathic sciatic nerve injury occurred in only one case. Conclusions： The sciatic nerve should be routinely exposed and protected during the surgery. The type of fixation should be based on the fracture type and site. Prolonged immobilization may be helpful in improving the final outcomes.
文摘Objective：To study the surgical treatment for distal humeral fractures in adults according to the follow-up results.Methods： Twenty-one cases （16 males and 5 females）of distal humeral fracture were included in this study. The average age was 42.5 years （range： 37-52 years）. Fractures were classified according to the AO classification system.Nine cases of C1,8 C2 and 4 C3-type fractures were identified.Open reduction and internal fixation were performed in all cases. A tricep-reflecting approach was adopted, and either the AO orthogonal plating or parallel plating technique was chosen,based on the fracture type. The plaster cast was removed 3 weeks after operation. Rehabilitation was encouraged during this period and afterwards. The average follow-up time was 12.2 months （range： 8-28 months）. The outcome was scored according to Aitken and Rorabeek system.Results：No nerve injury,nonunion or failure of fixation was encountered during the operation and follow-up.However, ossifying myosifis occurred in one case.Conclusions：A triceps-reflecting approach can provide adequate exposure to the joint. The use of AO orthogohal plating or parallel plating techniques based on the type of fractures can provide rigid fixation for the fracture.
文摘Posterolateral rotatory instability of the elbow describes a condition that radial head subluxation or dislocation occurs when forearm rotates externally in relation to humerus. It is difficult to diagnose and treat. We reported a typical case which was confirmed by physical examination and MR images. Ligamentous insufficiency was confirmed under direct vision, and was reconstructed with triceps fascia as described by Gong et al with slight modification. Regain of full function was achieved one year after surgery.
