丹参和钙通道拮抗剂抗LDL氧化作用的对比研究

提取健康成人血浆中的低密度脂蛋白（ＬＤＬ），与丹参和硝苯吡啶、尼索地平及Ｃｕ透析，分别于透析后２４、４８及７２小时从各组ＬＤＬ中提取脂类进行薄层层析（ＴＬＣ）。结果显示：①ｃｕ可使ＬＤＬ氧化成ｏｘＬＤＬ，ＴＬＣ示ｘ带、游离脂肪酸（ＦＦＡ）、磷脂（ＰＬ）增加，而胆固醇酯（ＣＥ）及甘油三酯（ＴＧ）减少；②钙通道拮抗剂＋Ｃｕ透析与单纯Ｃｕ透析结果相似；③丹参＋Ｃｕ透析的ｘ带显著低于Ｃｕ透析组及钙通道拮抗剂组，且脂质成分与透析前相似。结果表明，Ｘ带可作为脂质过氧化的一个重要指标，丹参具有拮抗ＬＤＬ氧化的作用，这可能是其防治心脑血管病的重要机理之一。

卒中部位及局部脑血流量对智力的影响

用长谷川智力测定量表法测定了１８９例卒中患者的智力，分析了病变部位、脑萎缩及局部脑血流量（ｒＣＢＦ）对智力的影响．其中６９例患者长谷川分数低于２１．５分．当病变位于左半球时，属痴呆或可疑痴呆者达４２．９％；而病变位于右半球时，仅２３．１％属痴呆及可疑痴呆．ｒＣＢＦ与长谷川分数之间的相关分析显示：左额、右颞及左顶叶的ｒＣＢＦ与长谷川分数呈极显著正相关．提示左半球在智力活动中起主导作用．脑萎缩亦是影响智力的因素．

从死亡诊断书看猝死

樱井依据WHO规定的国际疾病分类指出,所谓猝死(sudden death),是指原因不明的、无疾病症状、从发病到死亡最多不超过24小时的死亡。但问题是既往虽有心绞痛等,突然死亡时可否称为猝死。河合等人认为此种死亡应称为心脏性猝死(sudden cardiac death) 因脑卒中而死时,往往因出现偏瘫或突然打鼾,可以大致推测出来。但心脏病致死时,除医生外即使当时有人在场,许多事情往往也搞不清楚。实际上,在死亡诊断书中,以急性心功不全或心功不全为死亡病名的记载极多。这是因为多数在往诊时已经死亡。从真正死因的观点分析看可能还存在其他死因。作者为了研究心功不全或不明死亡,以青森县八户市为中心,根据死亡诊断书进行了猝死的实况调查。

THE EFFECT OF RADIX SALVIAE MILTIORRHIZAE COMPOSITA AND LIGUSTRAZINE ON LIPID PEROXIDATION IN LOW DENSITY LIPOPROTEIN DUE TO COPPER DICHLORIDE

It is well known that lipid peroxide in low density lipoprotein (LDL) plays an important role in atherosclerosis and atherosclerotic diseases. We used oxidized LDL generated by incu-bating LDL from healthy persons with copper dichloride as a model to investigate the anti-lipid-peroxide property of Radix Salviae Miltiorrhizae Composita (RSMC) and ligustrazine. The changes in concentrations of lipid peroxide and lipids in LDL due to Cu++ were studied, and the effects of RSMC and ligustrazine on the changes were studied. The re-sults in our study indicate that RSMC has a potential role on and-lipid-peroxidation, but it was not found that ligustrazine has similar anti-peroxidation action.

THE EFFECT OF RADIX SALVIAE MILTIORRHIZAE COMPOSITA ON PEROXIDATION OF LOW DENSITY LIPOPROTEIN DUE TO COPPER DICHLORIDE

It is well known that plasma lipoprotein, particularly oxidized LDL, plays an important rolein the pathogenesis of atherosclerosis and atherosclerotic diseases. We used oxidized LDLgenerated by incubating LDL from healthy Persons with copper dichloride as a model to in-vestigate the antioxidate property of Radix Salviae Miltiorrhizae Composita (RSMC). Onphotos, the spot X1 and the spot X2 were clearly found in the control group after the dislysisinto copper dichloride for 24 and 48 hours, but they could not found in the RSMC group.The analysis of the constituents of lipids in LDL (by charring method) showed that afterdislysis the percentages of the spot X1 and the spot X2 in the RSMC group weresignificantly lower tban those in the control group (P【 0.01). The results suggest thatRSMC plays a potential role in antioxidation of lipuls or LDL.

氯化铜对低密度脂蛋白及其脂质成分的影响以及临床实用价值

目前认为氧化ＬＤＬ是导致动脉硬化的重要危险因素之一。为研究氧化ＬＤＬ及其脂质的变化，本文作者将１１例健康人的ＬＤＬ置于５μＭＣｕＣｌ_２，溶液中透析，并用ＴＬＣ观察脂质成分的变化。结果为ＣＥ、ＴＧ随透析时间的延长而降低，ＦＦＡ、ＦＣ及ＰＬ则随透析时间的延长而升高，并在透析后２４小时出现明显的、代表过氧化ＣＥ的异常着色点Ｘ_１及Ｘ_２点。由于该实验方法的条件易于控制，重复性好，故为选择性测定过氧化ＣＥ的良好方法，可用于临床研究，分析ＣＶＤ时氧化ＬＤＬ中脂类及过氧化脂质的变化。此外尚可用于研究抗氧化ＬＤＬ及抗过氧化脂质药物。

巴曲酶促进小鼠腹腔巨噬细胞吞噬过氧化低密度脂蛋白

采用小鼠腹腔巨噬细胞在含有过氧化低密度脂蛋白（Ｐｏｘ－ＬＤＬ）的培养液中培养，并用巴曲酶（ｂａｔｒｏｘｏｂｉｎ）进行干预，运用透射电子显微镜进行观察，旨在了解巴曲酶对巨噬细胞吞噬ｐｏｘ－ＬＤＬ的影响。研究结果表明；小鼠腹腔巨噬细胞与纯培养剂ＤＭＥＭ正常低密度脂蛋白（ｎ－ＬＤＬ），以及ｎ－ＬＤＬ加巴曲酶培养４小时后，细胞的超微结构与培养前相似，而与Ｐｏｘ－ＬＤＬ一起培养４小时后细胞胞浆内则含大量的脂滴，加入巴曲酶及小鼠腹腔巨噬细胞内脂滴的生成明显加快。