Microtubule-associated proteins （MAPs） are important proteins in cells. They can regulate the organization, dynamics and function of microtubules. We measure the binding force between microtubule and a new plant MAP...Microtubule-associated proteins （MAPs） are important proteins in cells. They can regulate the organization, dynamics and function of microtubules. We measure the binding force between microtubule and a new plant MAP, i.e. AtMAP65-1, by dual-optical tweezers. The force is obtained to be 14.6 ± 3.5 pN from the data statistics and analysis. This force measurement is helpful to understand the function and mechanism of MAPs from the mechanical point of view and lays the groundwork for future measurements of the mechanical properties of other biological macro-molecules.展开更多
We set up a system of multiple optical tweezers based on a spatial light modulator,and measured the displacement and optical force of the trapped particles simultaneously.All of the trapped particles can be clearly im...We set up a system of multiple optical tweezers based on a spatial light modulator,and measured the displacement and optical force of the trapped particles simultaneously.All of the trapped particles can be clearly imaged in three dimensions by several CCDs.The displacement is obtained by calculating the gray weighted centroid in the trapped particle's image.The stiffness of the trapped particles in the optical traps is measured by oscillating the sample stage in a triangular wave based on Stokes fluid dynamics.The optical force of each trapped particle can be calculated by the measured displacement and stiffness.展开更多
基金Supported by the National Key Basic Research Programme of China under Grant No 2002CB713805, and the National Natural Science Foundation of China under Grant Nos 10374112 and 10604065.
文摘Microtubule-associated proteins （MAPs） are important proteins in cells. They can regulate the organization, dynamics and function of microtubules. We measure the binding force between microtubule and a new plant MAP, i.e. AtMAP65-1, by dual-optical tweezers. The force is obtained to be 14.6 ± 3.5 pN from the data statistics and analysis. This force measurement is helpful to understand the function and mechanism of MAPs from the mechanical point of view and lays the groundwork for future measurements of the mechanical properties of other biological macro-molecules.
基金Supported by the National Natural Science Foundation of China under Grant No 11104342the National Basic Research Foundation of China under Grant No 2011CB922002.
文摘We set up a system of multiple optical tweezers based on a spatial light modulator,and measured the displacement and optical force of the trapped particles simultaneously.All of the trapped particles can be clearly imaged in three dimensions by several CCDs.The displacement is obtained by calculating the gray weighted centroid in the trapped particle's image.The stiffness of the trapped particles in the optical traps is measured by oscillating the sample stage in a triangular wave based on Stokes fluid dynamics.The optical force of each trapped particle can be calculated by the measured displacement and stiffness.
