对103例颧部褐青色痣(NFZ)患者采用Q开关Na:YAG激光(波长1064 nm)治疗,每次治疗前记录并拍摄患者的皮损情况,两名皮肤科医生对患者进行Melasma Area Severity Index(MASI)评分,采用自身对照进行治疗前后疗效对比观察。基本治愈55例,基愈...对103例颧部褐青色痣(NFZ)患者采用Q开关Na:YAG激光(波长1064 nm)治疗,每次治疗前记录并拍摄患者的皮损情况,两名皮肤科医生对患者进行Melasma Area Severity Index(MASI)评分,采用自身对照进行治疗前后疗效对比观察。基本治愈55例,基愈率53.4%,总有效率89.3%,无效11例(均治疗1次),疗效与治疗次数成正相关。Q开关Na:YAG激光治疗颧部褐青色痣疗效满意,治疗安全,是该病的最佳治疗手段。展开更多
Summary： Endothelin-3 （ET-3） is aberrantly expressed in both metastatic melanoma tissues and cul tured melanoma cells. Our previous work showed that ET-3 could promote survival of metastatic mela noma cells via its...Summary： Endothelin-3 （ET-3） is aberrantly expressed in both metastatic melanoma tissues and cul tured melanoma cells. Our previous work showed that ET-3 could promote survival of metastatic mela noma cells via its altered expression. In this study, we investigated the mechanisms responsible for these gene-induced phenotypes in melanoma cells. An ET-3 gene sequence-specific shRNA vector pLVTHM-ET3-RNAi was constructed and transfected into human malignant melanoma cells A375 and MMRU, and the resultant molecular events and cellular changes were examined. As compared with the empty-vector group, cell proliferation was slowed down, and the growth inhibition rates were 38.9% in A375 cells and 38.4% in MMRU cells after transfection. In addition, cell invasion capability was also inhibited, with a reduction of 62.2% in A375 cells and 54.3% in MMRU cells. The percentage of apoptotic cells was found to increase. Meanwhile, in both cell lines, secreted protein acidic and rich in cy teine （SPARC） levels were down-regulated together with inhibition of its upstream signaling molecule, NF-kB. Thus, the current results suggested that down-regulated expression of ET3 attenuates the ma lignant behaviors of human melanoma ceils partially by decreasing the expression of SPARC and NF-kB.展开更多
文摘对103例颧部褐青色痣(NFZ)患者采用Q开关Na:YAG激光(波长1064 nm)治疗,每次治疗前记录并拍摄患者的皮损情况,两名皮肤科医生对患者进行Melasma Area Severity Index(MASI)评分,采用自身对照进行治疗前后疗效对比观察。基本治愈55例,基愈率53.4%,总有效率89.3%,无效11例(均治疗1次),疗效与治疗次数成正相关。Q开关Na:YAG激光治疗颧部褐青色痣疗效满意,治疗安全,是该病的最佳治疗手段。
基金supported partially by the grants from the National Natural Science Foundation of China(No.30671891,and No.81072232)the Canadian Foundation for Innovation,and the Canadian Institutes of Health Research(No.CIHR #IMH-37565 and No.MOP-84300)
文摘Summary： Endothelin-3 （ET-3） is aberrantly expressed in both metastatic melanoma tissues and cul tured melanoma cells. Our previous work showed that ET-3 could promote survival of metastatic mela noma cells via its altered expression. In this study, we investigated the mechanisms responsible for these gene-induced phenotypes in melanoma cells. An ET-3 gene sequence-specific shRNA vector pLVTHM-ET3-RNAi was constructed and transfected into human malignant melanoma cells A375 and MMRU, and the resultant molecular events and cellular changes were examined. As compared with the empty-vector group, cell proliferation was slowed down, and the growth inhibition rates were 38.9% in A375 cells and 38.4% in MMRU cells after transfection. In addition, cell invasion capability was also inhibited, with a reduction of 62.2% in A375 cells and 54.3% in MMRU cells. The percentage of apoptotic cells was found to increase. Meanwhile, in both cell lines, secreted protein acidic and rich in cy teine （SPARC） levels were down-regulated together with inhibition of its upstream signaling molecule, NF-kB. Thus, the current results suggested that down-regulated expression of ET3 attenuates the ma lignant behaviors of human melanoma ceils partially by decreasing the expression of SPARC and NF-kB.
