Genetic Diversity in Main Cultivars of Safflower in Xinjiang Uighur Autonomous Region Based on RAPD Analysis

[Objective] Study on the genetic diversity in main cultivars of safflower distributing in Xinjiang Uighur Autonomous Region by means of RAPD makers.[Method] Genomic DNAs of 29 safflower accessions from Xinjiang Uighur Autonomous Region were extracted for PCR amplification using 20 RAPD primers.[Result] Totally 156 bands were amplified,among which 144 bands were polymorphic(accounting for 92.31%),indicating that safflower is endowed with plentiful genetic diversity.Based on the DNA fingerprint,the 29 safflower accessions were grouped into four populations,the classification results may be not related with ecological regionality.[Conclusion] RAPD technique is an available tool to analyze the genetic diversity of safflower germplasm at molecular level.

新疆红花主要栽培品种遗传多样性的RAPD分析

[目的]利用RAPD技术分析新疆红花栽培品种遗传多样性。[方法]提取29份新疆红花栽培品种的基因组DNA并利用所筛选的20条RAPD引物进行PCR扩增。[结果]共扩增出156条带,其中144条带具有多态性,多态性条带占92.31%,说明新疆的红花具有较丰富的遗传多样性;根据引物扩增出的DNA指纹图谱,运用UPGMA分析法可将29份红花种质聚类划分为4个主要类群,该类群划分结果与红花的生态地域性可能不存相关性。[结论]该方法可用于在分子水平上分析红花品种的遗传多样性。

用RAPD和ISSR法研究新疆红花主栽品种的遗传多样性

【目的】对29份新疆红花栽培品种的遗传多样性进行检测。【方法】RAPD和ISSR分子标记技术。【结果】20个RAPD引物和18个ISSR引物分别扩增出156和112条带,多态条带比率（PPB）分别为92.31%和93.77%。RAPD和ISSR检测的有效等位基因数分别为1.508 1和1.513 7,基因多样性为0.3115和0.3416,Shannon多样性指数为0.4738和0.4798。以Nei氏遗传距离矩阵按UPGMA方法聚类分析结果RAPD和ISSR标记的遗传距离分别为0.108 2~2.0541和0.123 4~2.1535。【结论】红花不同品种之间具有比较丰富的遗传变异。对比RAPD和ISSR在PCR反应中的稳定性和检测变异的能力表明,对于实验条件的稳定性而言ISSR优于RAPD,且总的来说ISSR能检测到比RAPD更多的遗传变异。Mantel检测表明：这两种标记的分析结果有极显著的相关性r=0.963。

植物体细胞胚胎研究进展及研究基础

本文简述了1958年发现植物体细胞胚胎以来显微结构、超微结构的细胞学和组织化学等理化特性研究进展,同时还简述了体细胞胚发生时蛋白质、氨基酸代谢,淀粉粒动态、过氧化物酶同工酶变化、酯酶同工酶变化等基础研究。