目的:探讨环磷酸腺苷反应元件结合蛋白(cAMP response element binding protein,p-CREB)在切割痛敏形成中的作用。方法:异氟烷麻醉大鼠后切割后足。免疫组织化学染色,荧光双标对p-CREB进行定位。腹腔注射吗啡和加巴喷丁,进行行为学测定...目的:探讨环磷酸腺苷反应元件结合蛋白(cAMP response element binding protein,p-CREB)在切割痛敏形成中的作用。方法:异氟烷麻醉大鼠后切割后足。免疫组织化学染色,荧光双标对p-CREB进行定位。腹腔注射吗啡和加巴喷丁,进行行为学测定和免疫组织化学染色。结果:大鼠后足切割痛可以使同侧腰段脊髓背角内p-CREB增加,切割后0.5~3 h内比较明显(P<0.05)。p-CREB增加主要定位于背角神经元。腹腔注射吗啡产生镇痛作用的同时,与未切割组相比,p-CREB没有出现增加(P>0.05)。加巴喷丁产生部分镇痛作用时,与未切割组相比,p-CREB增加(P<0.05)。结论:切割疼痛所致的同侧脊髓背角内p-CREB增加与切割疼痛和痛觉过敏密切相关。吗啡对切割疼痛的镇痛作用机制与抑制脊髓背角内CREB的磷酸化增加有关。加巴喷丁对切割疼痛的镇痛作用机制与抑制脊髓背角内CREB磷酸化关系不密切。展开更多
We report here on a diode-pumped pulsed mid-infrared laser source based on gas-filled hollow-core fibers(HCFs)towards an all-fiber structure by the tapering method. The pump laser is coupled into an acetylene-filled H...We report here on a diode-pumped pulsed mid-infrared laser source based on gas-filled hollow-core fibers(HCFs)towards an all-fiber structure by the tapering method. The pump laser is coupled into an acetylene-filled HCF through a tapered single-mode fiber. By precisely tuning the wavelength of the diode to match different absorption lines of acetylene near 1.5 μm, mid-infrared emission around 3.1–3.2 μm is generated. With 2 m HCFs and3 mbar acetylene gas, a maximum average power of 130 m W is obtained with a laser slope efficiency of ~24%.This work provides a potential scheme for all-fiber mid-infrared fiber gas lasers.展开更多
基金supported by the Outstanding Youth Science Fund Project of Hunan Provincial Natural Science Foundation(No.2019JJ20023)the National Natural Science Foundation of China(NSFC)(No.61705266)
文摘We report here on a diode-pumped pulsed mid-infrared laser source based on gas-filled hollow-core fibers(HCFs)towards an all-fiber structure by the tapering method. The pump laser is coupled into an acetylene-filled HCF through a tapered single-mode fiber. By precisely tuning the wavelength of the diode to match different absorption lines of acetylene near 1.5 μm, mid-infrared emission around 3.1–3.2 μm is generated. With 2 m HCFs and3 mbar acetylene gas, a maximum average power of 130 m W is obtained with a laser slope efficiency of ~24%.This work provides a potential scheme for all-fiber mid-infrared fiber gas lasers.