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拉曼光谱技术在病理诊断中的研究进展 被引量:5
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作者 王茜蒨 相里文婷 +2 位作者 腾格尔 崔旭泰 魏凯 《光谱学与光谱分析》 SCIE EI CAS CSCD 北大核心 2021年第4期1016-1022,共7页
拉曼光谱技术能够提供与物质特定分子结构相关的光谱信息,可用于识别生物组织微小的生化变异,具有快速、实时、无损、无需样本预处理等优点,在临床病理诊断领域极具应用前景。与常规组织病理学分析相比,拉曼光谱技术能够直接检测活体组... 拉曼光谱技术能够提供与物质特定分子结构相关的光谱信息,可用于识别生物组织微小的生化变异,具有快速、实时、无损、无需样本预处理等优点,在临床病理诊断领域极具应用前景。与常规组织病理学分析相比,拉曼光谱技术能够直接检测活体组织,简化了分析程序,缩短了诊断时间。人体病变组织的细胞分子组成和结构可能发生变化,这为拉曼光谱技术在组织病理诊断中的应用提供了检测依据。基于组织分子组成与结构的差异,结合机器学习和化学计量学方法,拉曼光谱技术可以提供客观的诊断信息,实现快速、低侵入的病理诊断。回顾了近十年来拉曼光谱技术在组织病理诊断中的研究进展,对取得的关键成果进行了总结,阐述了当前离体和活体应用拉曼光谱技术的一些关键问题。针对离体拉曼光谱检测,重点评估福尔马林固定石蜡包埋样本、冷冻样本和新鲜组织样本等离体样本的适用情况;阐述拉曼光谱数据收集的关键技术,包括适用光源、光谱范围,以及病理样本光谱采集的方式等。对于活体拉曼光谱检测,重点介绍了活体检测研究中拉曼光谱技术应用的两种形式:结合医用内窥镜进行体内检测,以及开放手术中的直接检测;综述了临床适用的拉曼系统,重点介绍了当前活体拉曼研究中应用的光纤探头。同时,文章也讨论了拉曼光谱数据的处理与分析方法,通过光谱预处理,特征提取与分类识别,构建拉曼光谱病理诊断模型,在小样本范围能够获得较好的诊断结果。考虑临床实际应用,仍需要不断优化分析方法,实现拉曼光谱与生化信息的关联,将样本个体差异的影响纳入分类模型中,以提升模型性能。文章对拉曼光谱应用于病理诊断中的关键问题进行了讨论,为进一步开展研究提供参考。未来需要更深入和广泛地开展离体和活体研究,以促进拉曼光谱技术在临床中的应用。 展开更多
关键词 拉曼光谱 疾病诊断 术中指导 机器学习
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Laser-induced breakdown spectroscopy for the classification of wood materials using machine learning methods combined with feature selection 被引量:1
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作者 崔旭泰 王茜蒨 +2 位作者 魏凯 腾格尔 徐向君 《Plasma Science and Technology》 SCIE EI CAS CSCD 2021年第5期117-125,共9页
In this paper,we explore whether a feature selection method can improve model performance by using some classical machine learning models,artificial neural network,k-nearest neighbor,partial least squares-discriminati... In this paper,we explore whether a feature selection method can improve model performance by using some classical machine learning models,artificial neural network,k-nearest neighbor,partial least squares-discrimination analysis,random forest,and support vector machine(SVM),combined with the feature selection methods,distance correlation coefficient(DCC),important weight of linear discriminant analysis(IW-LDA),and Relief-F algorithms,to discriminate eight species of wood(African rosewood,Brazilian bubinga,elm,larch,Myanmar padauk,Pterocarpus erinaceus,poplar,and sycamore)based on the laser-induced breakdown spectroscopy(LIBS)technique.The spectral data are normalized by the maximum of line intensity and principal component analysis is applied to the exploratory data analysis.The feature spectral lines are selected out based on the important weight assessed by DCC,IW-LDA,and Relief-F.All models are built by using the different number of feature lines(sorted by their important weight)as input.The relationship between the number of feature lines and the correct classification rate(CCR)of the model is analyzed.The CCRs of all models are improved by using a suitable feature selection.The highest CCR achieves(98.55...0.39)%when the SVM model is established from 86 feature lines selected by the IW-LDA method.The result demonstrates that a suitable feature selection method can improve model recognition ability and reduce modeling time in the application of wood materials classification using LIBS. 展开更多
关键词 laser-induced breakdown spectroscopy(LIBS) feature selection wood materials
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Distinguish Fritillaria cirrhosa and nonFritillaria cirrhosa using laser-induced breakdown spectroscopy
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作者 魏凯 崔旭泰 +2 位作者 腾格尔 Mohammad Nouman KHAN 王茜蒨 《Plasma Science and Technology》 SCIE EI CAS CSCD 2021年第8期161-166,共6页
As traditional Chinese medicines,Fritillaria from different origins are very similar and it is difficult to distinguish them.In this study,the laser-induced breakdown spectroscopy combined with learning vector quantiz... As traditional Chinese medicines,Fritillaria from different origins are very similar and it is difficult to distinguish them.In this study,the laser-induced breakdown spectroscopy combined with learning vector quantization(LIBS-LVQ)was proposed to distinguish the powdered samples of Fritillaria cirrhosa and non-Fritillaria cirrhosa.We also studied the performance of linear discriminant analysis,and support vector machine on the same data set.Among these three classifiers,LVQ had the highest correct classification rate of 99.17%.The experimental results demonstrated that the LIBS-LVQ model could be used to differentiate the powdered samples of Fritillaria cirrhosa and non-Fritillaria cirrhosa. 展开更多
关键词 laser-induced breakdown spectroscopy(LIBS) learning vector quantization chemometric models robustness of model
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