Objective： To investigate the dynamic changes of glycine （Gly） and taurine （Tau） in corpus striatum of rats with ischemia-reperfusion injury （IRI）, and the regulation effect of electroacupuncture on them. Meth...Objective： To investigate the dynamic changes of glycine （Gly） and taurine （Tau） in corpus striatum of rats with ischemia-reperfusion injury （IRI）, and the regulation effect of electroacupuncture on them. Methods： Thirty SD rats were randomly divided into sham surgery group, model group, and treatment group. Rats in the sham surgery group were operated without ischemia. Rats in the model group and treatment group were operated to make model of cerebral IRI. The ischemia status lasts 1.5 h, and reperfusion was performed for 3.75 h. Rats in the treatment group were treated with electroacupuncture at Fengchi （GB 20）. Neurologic deficit score （NDS） was used to evaluate the rats functions. Microdialysis and High Performance Liquid Chromatography technique were used to detect the changes of Gly and Tan in corpus striatum of rats. Results： Contents of Gly and Tan increased at 1.5 h after ischemia. Them decreased to level as same as those rats in the sham surgery group after reperfusion. The content of Gly in the model and treatment group increased again at 2-2.5 h after reperfusion, and then decreased to the same level of the sham surgery group. There was a third increase of Gly content in the treatment group at 2.75 h after reperfusion. Electroacupuncture treatment could delay the decrease of Tan content after reperfusion, and make it increase at 1.5, 2 and 2.75 h after reperfusion. The peak appeared in the last time. NDS in the model and treatment group were more than that in the sham surgery group, and lowered at 5.25 h after surgery. Effects in treatment group was better than that in the model group （P〈0.05）. Conclusion： IRI makes contents of Gly and Tau in corpus striatum increase at a special time. Electroacuptmcture treatment could increase contents of Gly and Tau at a special time after reperfusion, which maybe an important mechanism of protecting effects of electroacupuncture on the brain.展开更多
基金National Basic Research Program of China (973 Program, 2005CB523306)the Scientific and Technologic Fund of Shanghai (014319364)+1 种基金Shanghai Leading Academic Discipline Project (S30304)Shanghai Municipal Education Commission (06CZ044)
文摘Objective： To investigate the dynamic changes of glycine （Gly） and taurine （Tau） in corpus striatum of rats with ischemia-reperfusion injury （IRI）, and the regulation effect of electroacupuncture on them. Methods： Thirty SD rats were randomly divided into sham surgery group, model group, and treatment group. Rats in the sham surgery group were operated without ischemia. Rats in the model group and treatment group were operated to make model of cerebral IRI. The ischemia status lasts 1.5 h, and reperfusion was performed for 3.75 h. Rats in the treatment group were treated with electroacupuncture at Fengchi （GB 20）. Neurologic deficit score （NDS） was used to evaluate the rats functions. Microdialysis and High Performance Liquid Chromatography technique were used to detect the changes of Gly and Tan in corpus striatum of rats. Results： Contents of Gly and Tan increased at 1.5 h after ischemia. Them decreased to level as same as those rats in the sham surgery group after reperfusion. The content of Gly in the model and treatment group increased again at 2-2.5 h after reperfusion, and then decreased to the same level of the sham surgery group. There was a third increase of Gly content in the treatment group at 2.75 h after reperfusion. Electroacupuncture treatment could delay the decrease of Tan content after reperfusion, and make it increase at 1.5, 2 and 2.75 h after reperfusion. The peak appeared in the last time. NDS in the model and treatment group were more than that in the sham surgery group, and lowered at 5.25 h after surgery. Effects in treatment group was better than that in the model group （P〈0.05）. Conclusion： IRI makes contents of Gly and Tau in corpus striatum increase at a special time. Electroacuptmcture treatment could increase contents of Gly and Tau at a special time after reperfusion, which maybe an important mechanism of protecting effects of electroacupuncture on the brain.
