棕色固氮菌固氮酶钼铁蛋白结晶,经酒石酸解离后得到一个含钢铁小分子组分,与棕色固氮菌突变株uw_(45)无细胞提取液的重组比活为6.8nM Mo natom^(-1) min^(-1)。它是由二种物质组成的混合物,其分子量分别为2100和1850道尔顿,分子量为210...棕色固氮菌固氮酶钼铁蛋白结晶,经酒石酸解离后得到一个含钢铁小分子组分,与棕色固氮菌突变株uw_(45)无细胞提取液的重组比活为6.8nM Mo natom^(-1) min^(-1)。它是由二种物质组成的混合物,其分子量分别为2100和1850道尔顿,分子量为2100道尔顿的成分含钼铁。酒石酸处理后的沉淀,再用N—甲基甲酰胺抽提得到的含钼铁组分具有恢复突变株uw_(45)乙炔还原活性的能力。经纸层析鉴定与用Shah法制备的铁钼辅因子相类似。由于Shah和Smith法制备的两种铁钼辅因子还原乙炔和氰化钾的比活不同,而且分子量也有大小,说明这两种铁钼辅因子结构可能不尽相同。展开更多
In 1971, Slepko et al. and Syrtsova et al. observed the structure of nitrogenase MoFe-protein using the electron microscope, and inferred that the MoFe-protein is composed of 4 spherical sub-units. In 1974, Stasny et ...In 1971, Slepko et al. and Syrtsova et al. observed the structure of nitrogenase MoFe-protein using the electron microscope, and inferred that the MoFe-protein is composed of 4 spherical sub-units. In 1974, Stasny et al. demonstrated that the molecules of MoFe-protein are each composed of four spherical sub-units arranged into a 90 × 90 × 40 right-angle hexahedron. In 1974, we observed the paracrystal which is formed by the regular arrangement of the MoFe-protein molecules, and researched the configuration of the MoFe-protein molecules.展开更多
For the study of the structure and function of the nitrogenase MoFe-protein, data on its molecular weight and molybdenum, iron, sulfur contents are important, but there are wide diserepencies between the data given by
文摘棕色固氮菌固氮酶钼铁蛋白结晶,经酒石酸解离后得到一个含钢铁小分子组分,与棕色固氮菌突变株uw_(45)无细胞提取液的重组比活为6.8nM Mo natom^(-1) min^(-1)。它是由二种物质组成的混合物,其分子量分别为2100和1850道尔顿,分子量为2100道尔顿的成分含钼铁。酒石酸处理后的沉淀,再用N—甲基甲酰胺抽提得到的含钼铁组分具有恢复突变株uw_(45)乙炔还原活性的能力。经纸层析鉴定与用Shah法制备的铁钼辅因子相类似。由于Shah和Smith法制备的两种铁钼辅因子还原乙炔和氰化钾的比活不同,而且分子量也有大小,说明这两种铁钼辅因子结构可能不尽相同。
文摘In 1971, Slepko et al. and Syrtsova et al. observed the structure of nitrogenase MoFe-protein using the electron microscope, and inferred that the MoFe-protein is composed of 4 spherical sub-units. In 1974, Stasny et al. demonstrated that the molecules of MoFe-protein are each composed of four spherical sub-units arranged into a 90 × 90 × 40 right-angle hexahedron. In 1974, we observed the paracrystal which is formed by the regular arrangement of the MoFe-protein molecules, and researched the configuration of the MoFe-protein molecules.
文摘For the study of the structure and function of the nitrogenase MoFe-protein, data on its molecular weight and molybdenum, iron, sulfur contents are important, but there are wide diserepencies between the data given by
