Objective To explore the effect of Vaspin on adipose tissue macrophage polarization and its underlying mechanism.Methods Fifty male SD rats,aged 8 weeks,were chosen and randomly allocated into three groups:the normal ...Objective To explore the effect of Vaspin on adipose tissue macrophage polarization and its underlying mechanism.Methods Fifty male SD rats,aged 8 weeks,were chosen and randomly allocated into three groups:the normal control(NC),the type 2 diabetes(T2DM),and various concentrations of Vaspin intervention(V1:480 ng/kg,V2:960 ng/kg,V3:1440 ng/kg).Vaspin was administered via intraperitoneal injection for 8 weeks.Glucose tolerance and insulin sensitivity were evaluated via intraperitoneal glucose tolerance test(IPGTT),intraperitoneal insulintolerance test(IPITT)and hyperinsulinemic-euglycemcicclamp.Adipose tissue inflammation and macrophage polarization were assessed using immunofluorescence,RT-PCR and western bloting.Results After 8 weeks of intervention,there were no statistically significant differences in body weight and blood lipid levels among groups.IPGTT,IPITT,and hyperinsulinemic-euglycemic clamp experiments demonstrated that Vaspin intervention improved blood glucose and insulin sensitivity.展开更多
文摘Objective To explore the effect of Vaspin on adipose tissue macrophage polarization and its underlying mechanism.Methods Fifty male SD rats,aged 8 weeks,were chosen and randomly allocated into three groups:the normal control(NC),the type 2 diabetes(T2DM),and various concentrations of Vaspin intervention(V1:480 ng/kg,V2:960 ng/kg,V3:1440 ng/kg).Vaspin was administered via intraperitoneal injection for 8 weeks.Glucose tolerance and insulin sensitivity were evaluated via intraperitoneal glucose tolerance test(IPGTT),intraperitoneal insulintolerance test(IPITT)and hyperinsulinemic-euglycemcicclamp.Adipose tissue inflammation and macrophage polarization were assessed using immunofluorescence,RT-PCR and western bloting.Results After 8 weeks of intervention,there were no statistically significant differences in body weight and blood lipid levels among groups.IPGTT,IPITT,and hyperinsulinemic-euglycemic clamp experiments demonstrated that Vaspin intervention improved blood glucose and insulin sensitivity.
