Super resolution imaging capable of resolving details beyond the diffraction limit is highly desired in many scientific and application fields, including bio-medicine, nanomaterial science, and opto-electronic integra...Super resolution imaging capable of resolving details beyond the diffraction limit is highly desired in many scientific and application fields, including bio-medicine, nanomaterial science, and opto-electronic integration. Up to now, many different methods have been proposed, among which wide-field, label-free super resolution microscopy is indispensable due to its good applicability to diverse sample types, large field of view(FOV), and high imaging speed. In recent years,nanostructures have made a crucial contribution to the wide-field, label-free subdiffraction microscopy, with various working mechanisms and configuration designs. This review summarizes the recent applications of the nanostructures in the wide-field, label-free super resolution microscopy, with the emphasis on the designs of hyperlens with hyperbolic dispersion, microsphere with "nano-jets", and nanowire ring illumination microscopy based on spatial frequency shift effect. The bottlenecks of the current techniques and possible solutions are also discussed.展开更多
Multimode fiber(MMF)possesses intrinsic,ultrathin,noninvasive geometric characteristics and high mode density optical characteristics,having great potential in endoscopic imaging[1,2].A typical MMF endoscopic imaging ...Multimode fiber(MMF)possesses intrinsic,ultrathin,noninvasive geometric characteristics and high mode density optical characteristics,having great potential in endoscopic imaging[1,2].A typical MMF endoscopic imaging system involves a camera placed behind the fiber distal end to calibrate the transmission matrix(TM)of the fiber,and when imaging into narrow channels,such as bronchus,or performing insertion into brain tissues,the camera should be replaced by a single-pixel detector placed outside in a reflection mode to record the signal.A traditional MMF imaging system adopts the point scan mode,whose resolution is limited by the numerical aperture(NA)of the fiber,and the imaging speed is restricted by the refresh rate of the modulator and the Nyquist sampling law.The need for high resolution,high speed,and three-dimensional(3D)imaging for observation of biological processes,such as neural activity,blood flow detection,and cellular dynamics,calls for original imaging approaches.展开更多
The invention of the optical microscope is significant to the development of many aspects of science and technology,including novel materials,life sciences and medicine,for its superiorities in resolving fine structur...The invention of the optical microscope is significant to the development of many aspects of science and technology,including novel materials,life sciences and medicine,for its superiorities in resolving fine structures.But,restrained by the Abbe diffraction limit。展开更多
基金Project supported by the National Natural Science Foundation of China(Grant Nos.61735017 and 51672245)the Zhejiang Provincial Natural Science Foundation of China(Grant No.R17F050003)+4 种基金the National Key Basic Research Program of China(Grant No.2015CB352003)the Fundamental Research Funds for the Central Universities,Chinathe Program for Zhejiang Leading Team of S&T Innovation,Chinathe Cao Guangbiao Advanced Technology Program,ChinaFirst-class Universities and Academic Programs,China
文摘Super resolution imaging capable of resolving details beyond the diffraction limit is highly desired in many scientific and application fields, including bio-medicine, nanomaterial science, and opto-electronic integration. Up to now, many different methods have been proposed, among which wide-field, label-free super resolution microscopy is indispensable due to its good applicability to diverse sample types, large field of view(FOV), and high imaging speed. In recent years,nanostructures have made a crucial contribution to the wide-field, label-free subdiffraction microscopy, with various working mechanisms and configuration designs. This review summarizes the recent applications of the nanostructures in the wide-field, label-free super resolution microscopy, with the emphasis on the designs of hyperlens with hyperbolic dispersion, microsphere with "nano-jets", and nanowire ring illumination microscopy based on spatial frequency shift effect. The bottlenecks of the current techniques and possible solutions are also discussed.
基金supported by the National Natural Science Foundation of China(61735017,61822510,62020106002,and 62005250)the National Key Basic Research Program of China(2021YFC2401403)Major Scientific Research Project of Zhejiang Lab(2019MC0AD02)。
文摘Multimode fiber(MMF)possesses intrinsic,ultrathin,noninvasive geometric characteristics and high mode density optical characteristics,having great potential in endoscopic imaging[1,2].A typical MMF endoscopic imaging system involves a camera placed behind the fiber distal end to calibrate the transmission matrix(TM)of the fiber,and when imaging into narrow channels,such as bronchus,or performing insertion into brain tissues,the camera should be replaced by a single-pixel detector placed outside in a reflection mode to record the signal.A traditional MMF imaging system adopts the point scan mode,whose resolution is limited by the numerical aperture(NA)of the fiber,and the imaging speed is restricted by the refresh rate of the modulator and the Nyquist sampling law.The need for high resolution,high speed,and three-dimensional(3D)imaging for observation of biological processes,such as neural activity,blood flow detection,and cellular dynamics,calls for original imaging approaches.
基金supported by the National Natural Science Foundation of China(61822510,61735017,62005250,and 62020106002)the Key Research Project of Zhejiang Lab(2019MC0AD02)+1 种基金Key Technologies Research Project Supported by the Commission of Science Technology of Shanxi Province(20201102010)China Postdoctoral Science Foundation(2020M681952)。
文摘The invention of the optical microscope is significant to the development of many aspects of science and technology,including novel materials,life sciences and medicine,for its superiorities in resolving fine structures.But,restrained by the Abbe diffraction limit。